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Gene Expression Data
Assay Details
Assay
Reference: J:103131 Purcell P, et al., Pax6-dependence of Six3, Eya1 and Dach1 expression during lens and nasal placode induction. Gene Expr Patterns. 2005 Dec;6(1):110-8
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5910089
Gene symbol: Six3
Gene name: sine oculis-related homeobox 3
Antibody: SIX3 antibody
Results
Specimen 1A: embryonic day 9.5 (more )
Note: Double labeled: red - Six3; green - Pax6 (assay MGI:5910088).
Structure Level Pattern Image Note
TS15: lens pre-placode Strong Regionally restricted 1A Strong expression in prospective lens ectoderm.
Specimen 1B: embryonic day 10.5 (more )
Note: Double labeled: red - Six3; green - Pax6 (assay MGI:5910088).
Structure Level Pattern Image Note
TS17: lens pit Present 1B
TS17: surface ectoderm Present Regionally restricted 1B Expression maintained in ectoderm surrounding the lens pit.
Specimen 2A: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: lens pre-placode Present Regionally restricted 2A Expression in pre-placode lens ectoderm cells, primarily localized to nuclei.
Specimen 2B: embryonic day 9.5; Pax6Sey/Pax6Sey (more )
Structure Level Pattern Image Note
TS15: lens pre-placode Present Regionally restricted 2B Expression in pre-placode lens ectoderm cells, principally detected in cytoplasm.
Specimen 2C: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: surface ectoderm Present 2C
Specimen 2D: embryonic day 10.5; Pax6Sey/Pax6Sey (more )
Structure Level Pattern Image Note
TS17: optic cup Strong Regionally restricted 2D Robust expression in optic vesicle.
TS17: surface ectoderm Ambiguous 2D Expression in surface ectoderm was downregulated.
Specimen 2K: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: lens pre-placode Present Regionally restricted 2K Expression primarily localized to nucleus
Specimen 2L: embryonic day 9.5; Pax6Sey/Pax6Sey (more )
Structure Level Pattern Image Note
TS15: lens pre-placode Present Regionally restricted 2L Expression mainly in cytoplasm.
Specimen 3B: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: retina Present Regionally restricted 3B Expression in developing ciliary marginal zone.
TS19: lens vesicle Present 3B
TS19: pigmented retinal epithelium Present 3B
TS19: cornea epithelium Present 3B Expression in surface ectoderm that will form the cornea.
Specimen 3E: embryonic day 11.5; Dach1tm1Mrd/Dach1tm1Mrd (more )
Structure Level Pattern Image Note
TS19: cornea epithelium Present 3E No detectable change in expression with respect to wild-type.
TS19: lens vesicle Present 3E No detectable change in expression with respect to wild-type.
TS19: pigmented retinal epithelium Present 3E No detectable change in expression with respect to wild-type.
TS19: retina Present 3E No detectable change in expression with respect to wild-type.
Specimen 4A: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: nose Present Regionally restricted 4A Expression in prospective nasal ectoderm
Specimen 4B: embryonic day 9.5; Pax6Sey/Pax6Sey (more )
Structure Level Pattern Image Note
TS15: nose Present Regionally restricted 4B Expression indistinguishable from wild type embryos.
Specimen 4H: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: nasal epithelium Present 4H Expression throughout nasal ectoderm as nasal placode begins to invaginate to form the nasal pit.
Specimen 4I: embryonic day 10.5; Pax6Sey/Pax6Sey (more )
Structure Level Pattern Image Note
TS17: nasal epithelium Absent 4I No expression in prospective nasal ectoderm.
TS17: forebrain Present 4I Authors report that forebrain expression is preserved.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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06/12/2024
MGI 6.13 		The Jackson Laboratory