Gene Expression Data
Assay Details
Assay
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Reference:
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J:134595
Anthony TE, et al., The folate metabolic enzyme ALDH1L1 is restricted to the midline of the early CNS, suggesting a role in human neural tube defects. J Comp Neurol. 2007 Jan 10;500(2):368-83
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6149196
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Gene symbol:
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Aldh1l1
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Gene name:
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aldehyde dehydrogenase 1 family, member L1
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Results
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Specimen
1A:
embryonic day 9.5
(more )
Note: A TSA amplification step was used for detection.
Specimen
1A:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
A TSA amplification step was used for detection.
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: future hindbrain |
Present |
Regionally restricted |
1A
|
Expression is in cells flanking the ventral midline.
|
Specimen
1B:
embryonic day 9.5
(more )
Note: A TSA amplification step was used for detection.
Specimen
1B:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
A TSA amplification step was used for detection.
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: future hindbrain |
Present |
Regionally restricted |
1B
|
Expression is in cells flanking the ventral midline.
|
Specimen
1C:
embryonic day 10.5
(more )
Specimen
1C:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: hindbrain |
Present |
Regionally restricted |
1C
|
Expression is at the ventral midline.
|
Specimen
1D:
embryonic day 10.5
(more )
Specimen
1D:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: hindbrain |
Present |
Regionally restricted |
1D
|
Expression is at the ventral midline.
|
Specimen
1E,1F:
embryonic day 12.5
(more )
Note: Double labeled: red - Aldh1l1; green - Nes (assay MGI:6149221). F is a higher magnification of the center of panel E.
Specimen
1E,1F:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Aldh1l1; green - Nes (assay MGI:6149221). F is a higher magnification of the center of panel E.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Present |
Regionally restricted |
1E
,
1F
,
1H
|
Expression is at the dorsal and ventral midline.
|
Specimen
1I:
embryonic day 14.5
(more )
Specimen
1I:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: spinal cord |
Present |
Regionally restricted |
1I
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Expression is in radial glia.
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Specimen
1J:
embryonic day 14.5
(more )
Specimen
1J:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: tectum |
Present |
Regionally restricted |
1J
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Expression is in the midline radial glial cells.
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Specimen
1K:
embryonic day 14.5
(more )
Specimen
1K:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: hypothalamus |
Present |
Regionally restricted |
1K
|
Expression is in the midline radial glial cells.
|
Specimen
1L:
embryonic day 16.5
(more )
Specimen
1L:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
2O:
embryonic day 17.5
(more )
Specimen
2O:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: cerebral cortex |
Present |
|
2O
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Expression is throughout radial glial cell bodies and processes.
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Specimen
2Q:
embryonic day 17.5
(more )
Specimen
2Q:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: cerebral cortex |
Present |
|
2Q
|
Expression is throughout radial glial cell bodies and processes.
|
Specimen
3A:
embryonic day 12.5
(more )
Specimen
3A:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Present |
Regionally restricted |
3A
|
Expression is in radial glia flanking the ventral and dorsal midline.
|
Specimen
3B:
embryonic day 12.5
(more )
Specimen
3B:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
3C:
embryonic day 12.5
(more )
Specimen
3C:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
3D:
embryonic day 14.5
(more )
Specimen
3D:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
4A:
embryonic day 11.5
(more )
Specimen
4A:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: future spinal cord |
Present |
Regionally restricted |
4A
|
Expression is in a small population of radial glia flanking the floor plate.
|
Specimen
4C:
embryonic day 12.5
(more )
Specimen
4C:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Present |
Regionally restricted |
4C
|
Expression is in the ventral midline radial glia and is spreading to more dorsally located radial glia.
|
Specimen
4E:
embryonic day 12.5
(more )
Note: Double labeled: red - Aldh1l1; green - Pax3 (assay MGI:6149225).
Specimen
4E:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Aldh1l1; green - Pax3 (assay MGI:6149225).
|
Specimen
4H:
embryonic day 14.5
(more )
Specimen
4H:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: spinal cord |
Strong |
Regionally restricted |
4H
|
Expression is in radial glia throughout the dorsoventral axis.
|
Specimen
not shown E8.5:
embryonic day 8.5
(more )
Specimen
not shown E8.5:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
not shown E9.5:
embryonic day 9.5
(more )
Specimen
not shown E9.5:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: future spinal cord |
Present |
Regionally restricted |
|
Expression is in cells flanking the ventral midline of the cervical spinal cord.
|
|