Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:221192
Prasitsak T, et al., Foxc1 is required for early stage telencephalic vascular development. Dev Dyn. 2015 May;244(5):703-11
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:6154605
|
|
Gene symbol:
|
Pecam1
|
|
Gene name:
|
platelet/endothelial cell adhesion molecule 1
|
|
Antibody:
|
anti-CD31
|
|
Detection system:
|
Secondary antibody coupled to Alexa Fluor
|
|
|
Results
|
Specimen
1C:
embryonic day 11.5
(more  )
Note: Triple labeled: red - CD31 (Pecam1); green - Ter119 (Ly76 assay MGI:6154608); blue - Hoechst.
Specimen
1C:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Triple labeled: red - CD31 (Pecam1); green - Ter119 (Ly76 assay MGI:6154608); blue - Hoechst.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: telencephalon |
Present |
Regionally restricted |
1C
|
Expression in blood vessels.
|
Specimen
1D:
embryonic day 11.5; Foxc1ch/ Foxc1ch
(more )
Note: Triple labeled: red - CD31 (Pecam1); green - Ter119 (Ly76 assay MGI:6154608); blue - Hoechst.
Specimen
1D:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CHMU/Le
|
|
Mutant Allele(s):
|
Foxc1ch/Foxc1ch
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Triple labeled: red - CD31 (Pecam1); green - Ter119 (Ly76 assay MGI:6154608); blue - Hoechst.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: telencephalon |
Present |
Regionally restricted |
1D
|
Expression in blood vessels.
|
Specimen
E10.0 wt not shown:
embryonic day 10.5
(more )
Specimen
E10.0 wt not shown:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: telencephalon |
Present |
Regionally restricted |
|
Authors report invasion of endothelial cells into ventral telencephalon beginning at E10.0 along radial path from surrounding perinuclear vascular plexus.
|
Specimen
E10.0 ch/ch not shown:
embryonic day 10.5; Foxc1ch/ Foxc1ch
(more )
Specimen
E10.0 ch/ch not shown:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CHMU/Le
|
|
Mutant Allele(s):
|
Foxc1ch/Foxc1ch
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: telencephalon |
Present |
Regionally restricted |
|
Authors report invasion of endothelial cells into ventral telencephalon seems to be slightly delayed compared with wild type.
|
Specimen
1E:
embryonic day 11.0
(more )
Note: Triple labeled: red - CD31 (Pecam1); green - NG2 (Cspg4 assay MGI:6154609); blue - Hoechst.
Specimen
1E:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Triple labeled: red - CD31 (Pecam1); green - NG2 (Cspg4 assay MGI:6154609); blue - Hoechst.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS18: telencephalon |
Present |
Regionally restricted |
1E
|
Authors report endothelial cells reaching subventricular zone (SVZ) where branching began to form the subventricular vascular plexus (SVP).
|
Specimen
1F:
embryonic day 11.0; Foxc1ch/ Foxc1ch
(more )
Note: Triple labeled: red - CD31 (Pecam1); green - NG2 (Cspg4 assay MGI:6154609); blue - Hoechst.
Specimen
1F:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CHMU/Le
|
|
Mutant Allele(s):
|
Foxc1ch/Foxc1ch
|
|
Age:
|
embryonic day 11.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Triple labeled: red - CD31 (Pecam1); green - NG2 (Cspg4 assay MGI:6154609); blue - Hoechst.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS18: telencephalon |
Present |
Regionally restricted |
1F
|
Expression in endothelial cells reaching the subventricular zone and starting to branch.
|
Specimen
1G:
embryonic day 11.5
(more )
Note: Triple labeled: red - CD31 (Pecam1); green - NG2 (Cspg4 assay MGI:6154609); blue - Hoechst.
Specimen
1G:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Triple labeled: red - CD31 (Pecam1); green - NG2 (Cspg4 assay MGI:6154609); blue - Hoechst.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: telencephalon |
Present |
Regionally restricted |
1G
|
Authors report subventricular vascular plexus formation.
|
Specimen
1H:
embryonic day 11.5; Foxc1ch/ Foxc1ch
(more )
Note: Triple labeled: red - CD31 (Pecam1); green - NG2 (Cspg4 assay MGI:6154609); blue - Hoechst.
Specimen
1H:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CHMU/Le
|
|
Mutant Allele(s):
|
Foxc1ch/Foxc1ch
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Triple labeled: red - CD31 (Pecam1); green - NG2 (Cspg4 assay MGI:6154609); blue - Hoechst.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: telencephalon |
Present |
Regionally restricted |
1H
|
Authors report abnormal subventricular vascular plexus formation and glomerular-like endothelial cell aggregation.
|
Specimen
1I:
embryonic day 11.5
(more )
Note: Double labeled: red - CD31 (Pecam1); blue - Hoechst.
Specimen
1I:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - CD31 (Pecam1); blue - Hoechst.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: telencephalon |
Present |
Regionally restricted |
1I
|
Authors report blood vessels in dorsal hindbrain begin plexus formation in the subventricular zone.
|
Specimen
1J:
embryonic day 11.5; Foxc1ch/ Foxc1ch
(more )
Note: Double labeled: red - CD31 (Pecam1); blue - Hoechst.
Specimen
1J:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CHMU/Le
|
|
Mutant Allele(s):
|
Foxc1ch/Foxc1ch
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - CD31 (Pecam1); blue - Hoechst.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: telencephalon |
Present |
Regionally restricted |
1J
|
Authors report blood vessels in dorsal hindbrain begin plexus formation in the subventricular zone, with no evidence of vessel dilation.
|
Specimen
3H':
embryonic day 11.0
(more )
Note: Triple labeled: red - CD31 (Pecam1); green - Pdgfr (Pdgfrb assay MGI:6154615); blue - Topro3.
Specimen
3H':
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Triple labeled: red - CD31 (Pecam1); green - Pdgfr (Pdgfrb assay MGI:6154615); blue - Topro3.
|
Specimen
4A/B':
embryonic day 11.5
(more )
Note: Triple labeled: red - CD31 (Pecam1); green - Vegfr-2 (Kdr assay MGI:6154611); blue - Hoechst.
Specimen
4A/B':
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Triple labeled: red - CD31 (Pecam1); green - Vegfr-2 (Kdr assay MGI:6154611); blue - Hoechst.
|
Specimen
4C/D':
embryonic day 11.5; Foxc1ch/ Foxc1ch
(more )
Note: Triple labeled: red - CD31 (Pecam1); green - Vegfr-2 (Kdr assay MGI:6154611); blue - Hoechst.
Specimen
4C/D':
(close )
|
Genetic Background:
|
involves: C57BL/6 * CHMU/Le
|
|
Mutant Allele(s):
|
Foxc1ch/Foxc1ch
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Triple labeled: red - CD31 (Pecam1); green - Vegfr-2 (Kdr assay MGI:6154611); blue - Hoechst.
|
Specimen
5A/B:
embryonic day 11.0
(more )
Note: Triple labeled: red - CD31 (Pecam1); green - Collagen IV; blue - Hoechst.v
Specimen
5A/B:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Triple labeled: red - CD31 (Pecam1); green - Collagen IV; blue - Hoechst.v
|
Specimen
5C/D:
embryonic day 11.0; Foxc1ch/ Foxc1ch
(more )
Note: Triple labeled: red - CD31 (Pecam1); green - Collagen IV; blue - Hoechst.
Specimen
5C/D:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CHMU/Le
|
|
Mutant Allele(s):
|
Foxc1ch/Foxc1ch
|
|
Age:
|
embryonic day 11.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Triple labeled: red - CD31 (Pecam1); green - Collagen IV; blue - Hoechst.
|
Specimen
5G/H/I:
embryonic day 11.5
(more )
Note: Triple labeled: red - CD31 (Pecam1); green - Collagen IV; blue - Hoechst.
Specimen
5G/H/I:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Triple labeled: red - CD31 (Pecam1); green - Collagen IV; blue - Hoechst.
|
Specimen
5J/K/L:
embryonic day 11.5; Foxc1ch/ Foxc1ch
(more )
Note: Triple labeled: red - CD31 (Pecam1); green - Collagen IV; blue - Hoechst.
Specimen
5J/K/L:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CHMU/Le
|
|
Mutant Allele(s):
|
Foxc1ch/Foxc1ch
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Triple labeled: red - CD31 (Pecam1); green - Collagen IV; blue - Hoechst.
|
Specimen
5R/S:
embryonic day 11.5
(more )
Note: Triple labeled: red - CD31 (Pecam1); green - Laminin; blue - Hoechst.
Specimen
5R/S:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Triple labeled: red - CD31 (Pecam1); green - Laminin; blue - Hoechst.
|
Specimen
5T/U:
embryonic day 11.5; Foxc1ch/ Foxc1ch
(more )
Note: Triple labeled: red - CD31 (Pecam1); green - Laminin; blue - Hoechst.
Specimen
5T/U:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CHMU/Le
|
|
Mutant Allele(s):
|
Foxc1ch/Foxc1ch
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Triple labeled: red - CD31 (Pecam1); green - Laminin; blue - Hoechst.
|
|
Analysis Tools