Results
|
Specimen
2M:
embryonic day 12.5; Kifbpem1Hmy/Kifbp +
(more )
Specimen
2M:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Kifbpem1Hmy/Kifbp+
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: small intestine |
Present |
Regionally restricted |
2M
|
Expression was seen in neurites.
|
Specimen
2N:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Kifbpem1Hmy/Kifbpem1Hmy
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: small intestine |
Present |
Regionally restricted |
2N
|
Expression was detected in neurites similar to in wild type.
|
Specimen
2O:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Kifbpem1Hmy/Kifbpem1Hmy
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Note:
|
Double labeled: red - Tubb3; green - Sox10 (assay MGI:6160844).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: large intestine |
Present |
Regionally restricted |
2O
|
Expression in neurites in the mid-colon Sox10 immunopositive neural crest cell wavefront was variable. In this sample, very few Tubb3 immunopositive neurites were detected.
|
Specimen
2P:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Kifbpem1Hmy/Kifbpem1Hmy
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Note:
|
Double labeled: red - Tubb3; green - Sox10 (assay MGI:6160844).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: large intestine |
Present |
Regionally restricted |
2P
|
Expression in neurites in the mid-colon Sox10 immunopositive neural crest cell wavefront was variable. In this sample, most neurites at the wavefront were Tubb3 immunopositive.
|
Specimen
4A/A':
embryonic day 12.5
(more )
Note: Double labeled: red - Tubb3; green - Sox10 (assay MGI:6160844).
Specimen
4A/A':
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Note:
|
Double labeled: red - Tubb3; green - Sox10 (assay MGI:6160844).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: stomach |
Present |
Regionally restricted |
4A
,
4A'
|
Expression was detected in the vagus nerve at the gastroesophageal junction and as vagal nerve fibers extended into the stomach and then branched into smaller bundles.
|
TS20: vagus nerve |
Present |
Regionally restricted |
4A
,
4A'
|
Expression was detected in the vagus nerve at the gastroesophageal junction and as vagal nerve fibers extended into the stomach and then branched into smaller bundles.
|
Specimen
4B/B':
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Kifbpem1Hmy/Kifbpem1Hmy
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Note:
|
Double labeled: red - Tubb3; green - Sox10 (assay MGI:6160844).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: stomach |
Present |
Regionally restricted |
4B
,
4B'
|
Expression was detected in the vagus nerve at the gastroesophageal junction and as vagal nerve fibers extended into the stomach and then branched into smaller bundles. Phenotypic abnormalities were noted.
|
TS20: vagus nerve |
Present |
Regionally restricted |
4B
,
4B'
|
Expression was detected in the vagus nerve at the gastroesophageal junction and as vagal nerve fibers extended into the stomach and then branched into smaller bundles. Phenotypic abnormalities were noted.
|
Specimen
4E/E':
(close )
Genetic Background:
|
involves: 129X1/SvJ
|
Mutant Allele(s):
|
Rettm1Jmi/Rettm1Jmi
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Note:
|
Double labeled: red - Tubb3; green - Sox10 (assay MGI:6160844).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: stomach |
Present |
Regionally restricted |
4E'
,
4E
|
Expression was detected in the vagus nerve at the gastroesophageal junction. Vagal nerve fibers were detected only in the proximal stomach. Phenotypic abnormalities in vagal branches were noted.
|
TS20: vagus nerve |
Present |
Regionally restricted |
4E'
,
4E
|
Expression was detected in the vagus nerve at the gastroesophageal junction. Vagal nerve fibers were detected only in the proximal stomach. Phenotypic abnormalities in vagal branches were noted.
|
Specimen
4F/F':
(close )
Structure |
Level |
Pattern |
Image |
Note |
TS20: vagus nerve |
Present |
Regionally restricted |
4F
,
4F'
|
Expression was detected in the vagus nerve at the gastroesophageal junction.
|
TS20: stomach |
Present |
Regionally restricted |
4F
,
4F'
|
In 2/4 double mutant mice, vagal nerve fibers were absent from both sides of the stomach, and the only immunostaining was associated with a very small number of intrinsic neurons. In the other 2/4 double mutant mice, a small number of short vagal fibers was present on one side of the stomach only.
|
Specimen
4G:
postnatal day 0
(more )
Specimen
4G:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: stomach |
Present |
Regionally restricted |
4G
|
Expression was detected in the vagus nerve at the gastroesophageal junction and as vagal nerve fibers extended into the stomach and then branched into smaller bundles.
|
TS27: vagus nerve |
Present |
Regionally restricted |
4G
|
Expression was detected in the vagus nerve at the gastroesophageal junction and as vagal nerve fibers extended into the stomach and then branched into smaller bundles.
|
Specimen
4H:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Kifbpem1Hmy/Kifbpem1Hmy
|
Age:
|
postnatal day 0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: stomach |
Present |
Regionally restricted |
4H
|
Expression was detected in the vagus nerve at the gastroesophageal junction and as vagal nerve fibers extended into the stomach and then branched into smaller bundles. Phenotypic abnormalities were noted.
|
TS27: vagus nerve |
Present |
Regionally restricted |
4H
|
Expression was detected in the vagus nerve at the gastroesophageal junction and as vagal nerve fibers extended into the stomach and then branched into smaller bundles. Phenotypic abnormalities were noted.
|
Specimen
4I:
(close )
Genetic Background:
|
involves: 129X1/SvJ
|
Mutant Allele(s):
|
Kifbpem1Hmy/Kifbp+ Rettm1Jmi/Rettm1Jmi
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Note:
|
Double labeled: red - Tubb3; green - Sox10 (assay MGI:6160844).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: pancreas |
Present |
Regionally restricted |
4I
|
Immunopositive vagal fibers are present in the pancreas.
|
TS20: vagus nerve |
Present |
Regionally restricted |
4I
|
Immunopositive vagal fibers are present in the pancreas.
|
Specimen
4J:
(close )
Genetic Background:
|
involves: 129X1/SvJ
|
Mutant Allele(s):
|
Kifbpem1Hmy/Kifbpem1Hmy Rettm1Jmi/Ret+
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Note:
|
Double labeled: red - Tubb3; green - Sox10 (assay MGI:6160844).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: pancreas |
Present |
Regionally restricted |
4J
|
Small numbers of immunopositive vagal fibers are present in the pancreas.
|
TS20: vagus nerve |
Present |
Regionally restricted |
4J
|
Small numbers of immunopositive vagal fibers are present in the pancreas.
|
Specimen
4K:
(close )
Structure |
Level |
Pattern |
Image |
Note |
TS20: pancreas |
Absent |
|
4K
|
No vagal nerve fibers were observed in the pancreas.
|
Specimen
5A:
embryonic day 12.5
(more )
Note: Double labeled: red - Tubb3; green - Sox10 (assay MGI:6160844).
Specimen
5A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Note:
|
Double labeled: red - Tubb3; green - Sox10 (assay MGI:6160844).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: vagus nerve |
Present |
|
5A
|
|
TS20: lung |
Present |
Regionally restricted |
5A
|
Immunopositive vagal fibers were detected in the lung.
|
Specimen
5B:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Kifbpem1Hmy/Kifbpem1Hmy
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Note:
|
Double labeled: red - Tubb3; green - Sox10 (assay MGI:6160844).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: vagus nerve |
Present |
|
5B
|
|
TS20: lung |
Present |
Regionally restricted |
5B
|
Immunopositive vagal fibers were detected in the lung, but the number of fibers was reduced in the mutant.
|
Specimen
5C:
embryonic day 16.5; Kifbpem1Hmy/Kifbp +
(more )
Specimen
5C:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Kifbpem1Hmy/Kifbp+
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: diaphragm |
Present |
Regionally restricted |
5C
|
Immunopositive nerve terminals were present in the costal muscle of the diaphragm.
|
Specimen
5D:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Kifbpem1Hmy/Kifbpem1Hmy
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: diaphragm |
Present |
Regionally restricted |
5D
|
Immunopositive nerve terminals were present in the costal muscle of the diaphragm. There was no noticeable difference in the mutant compared to wild type.
|
Specimen
6M,6O:
postnatal day 0; Kifbpem1Hmy/Kifbp +
(more )
Specimen
6M,6O:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Kifbpem1Hmy/Kifbp+
|
Age:
|
postnatal day 0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: olfactory system |
Present |
Regionally restricted |
6M
,
6O
|
Immunopositive axons were observed projecting from the olfactory epithelium towards the olfactory bulbs.
|
Specimen
6N,6P:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Kifbpem1Hmy/Kifbpem1Hmy
|
Age:
|
postnatal day 0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: olfactory system |
Present |
Regionally restricted |
6P
,
6N
|
Immunopositive axons were observed projecting from the olfactory epithelium towards the olfactory bulbs. There was no obvious difference in the appearance of staining in the mutant.
|
Specimen
S3A top right:
embryonic day 10.5
(more )
Note: Double labeled: red - Tubb3; gray - Kifbp (assay MGI:6160842).
Specimen
S3A top right:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Gelatin
|
Note:
|
Double labeled: red - Tubb3; gray - Kifbp (assay MGI:6160842).
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: gut |
Present |
Regionally restricted |
S3A left
,
S3A top right
|
Expression was detected in neurons. There was overlap of immunopositive cells that hybridized to the Kif1bp RNA probe.
|
Specimen
S3B top right:
embryonic day 11.5
(more )
Note: Double labeled: red - Tubb3; gray - Kifbp (assay MGI:6160842).
Specimen
S3B top right:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Gelatin
|
Note:
|
Double labeled: red - Tubb3; gray - Kifbp (assay MGI:6160842).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: small intestine |
Present |
Regionally restricted |
S3B left
,
S3B top right
|
Expression was detected in neurons. There was overlap of immunopositive cells that hybridized to the Kif1bp RNA probe.
|
Specimen
S3D top right:
embryonic day 14.5
(more )
Note: Double labeled: red - Tubb3; gray - Kifbp (assay MGI:6160842).
Specimen
S3D top right:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Gelatin
|
Note:
|
Double labeled: red - Tubb3; gray - Kifbp (assay MGI:6160842).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: small intestine |
Present |
Regionally restricted |
S3D top right
,
S3D left
|
Expression was detected in neurons. There was overlap of immunopositive cells that hybridized to the Kif1bp RNA probe.
|
Specimen
S3E top right:
embryonic day 14.5
(more )
Note: Double labeled: red - Tubb3; gray - Kifbp (assay MGI:6160842).
Specimen
S3E top right:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Gelatin
|
Note:
|
Double labeled: red - Tubb3; gray - Kifbp (assay MGI:6160842).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: large intestine |
Present |
Regionally restricted |
S3E top right
,
S3E left
|
Expression was detected in neurons in the colon. There was overlap of immunopositive cells that hybridized to the Kif1bp RNA probe.
|
|