Antibody:	Anti-Calu (HPA006018)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS27: brain	Moderate	Widespread	4C
TS27: cerebellum	Strong		4C
TS27: cerebral cortex	Strong		4C
TS27: main olfactory bulb mitral cell layer	Strong		4C
TS27: forebrain ventricular layer	Present	Regionally restricted	4C	Expression was detected in migrating cells around the lateral ventricle.

Structure	Level	Pattern	Image	Note
TS27: forebrain ventricular layer	Present	Regionally restricted	4D	High immunoreactivity was detected in migrating cells and radial-like glia around the lateral ventricle.

Structure	Level	Pattern	Image	Note
TS28: brain	Moderate	Widespread	4E
TS28: cerebellum	Strong		4E
TS28: olfactory tract	Strong		4E
TS28: thalamic nucleus	Strong		4E

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		4F
TS28: deep cerebellar nucleus	Strong		4F

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		4G
TS28: cerebellum molecular layer	Strong		4G	Immunoreactive extensions from the Purkinje cell layer were seen throughout the molecular layer.
TS28: cerebellum granule cell layer	Present	Single cells	4G	Individual immunopositive cells were detected in the granule cell layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		4H , 4I	The cells in the Purkinje cell layer with the strongest intensity immunostaining for Calumenin were negative for Rbfox3 (NeuN) immunostaining. The immunopositive cells were not Purkinje cells, but most probably represent glia.
TS28: cerebellum granule cell layer	Present	Single cells	4H	Individual immunopositive cells were detected.
TS28: cerebellum molecular layer	Present	Single cells	4H	Individual immunopositive cells were detected.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		4J	The cells in the Purkinje cell layer with the strongest intensity immunostaining for Calumenin were negative for Calb1 immunostaining. The immunopositive cells were not Purkinje cells, but most probably represent glia.
TS28: cerebellum granule cell layer	Present	Single cells	4J	Individual immunopositive cells were detected in the granule cell layer.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb external plexiform layer	Moderate	Single cells	4K

Structure	Level	Pattern	Image	Note
TS28: pons	Present		4L	Calumenin and Rbfox3 co-localize in individual large neuronal cells bodies. There were also Rbfox3-negative small cells expressing Calumenin.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	Strong		4M	In the basal part of the granule cell layer, there are both Rbfox3-positive and -negative cells of diverse morphology strongly stained for Calumenin.
TS28: dentate gyrus molecular layer	Present	Single cells	4M	There are only small, individual Rbfox3-negative cells expressing Calumenin.
TS28: dentate gyrus polymorphic layer	Present	Single cells	4M	There are only small, individual Rbfox3-negative cells expressing Calumenin.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus subgranular zone	Present		4N	There are Calumenin-positive cells in the subgranular zone that co-express Gfap.
TS28: dentate gyrus granule cell layer	Present		4N	Expression was noted in astrocytes.
TS28: dentate gyrus molecular layer	Present		4N	Expression was noted in astrocytes.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus subgranular zone	Present		4O	There are Calumenin-positive cells in the subgranular zone that co-express Ncam1 (PSA-NCAM).

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus subgranular zone	Present	Single cells	4P	A large cell in the subgranular layer expressed both Calumenin and Rbfox3 (weakly red).
TS28: dentate gyrus granule cell layer	Present	Single cells	4Q , 4P	One cell at the border between the granular and molecular layers is immunopositive for both Calumenin and Rbfox3.
TS28: dentate gyrus molecular layer	Present	Single cells	4Q , 4P	One cell at the border between the granular and molecular layers is immunopositive for both Calumenin and Rbfox3.