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Gene Expression Data
Assay Details
Assay
Reference: J:170258 Mavromatakis YE, et al., Foxa1 and Foxa2 positively and negatively regulate Shh signalling to specify ventral midbrain progenitor identity. Mech Dev. 2011 Jan-Feb;128(1-2):90-103
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6343550
Gene symbol: Foxa1
Gene name: forkhead box A1
Antibody: Anti-Foxa1
Results
Specimen 1Ab: embryonic day 8.5 (more )
Note: Double labeled: red - Foxa1; green - Foxa2 (assay MGI:6343551).
Structure Level Pattern Image Note
TS13: future midbrain floor plate Present 1Ab Expression overlapped with Foxa2 to a large extent.
Specimen 1Ad: embryonic day 9.5 (more )
Note: Double labeled: red - Foxa1; green - Foxa2 (assay MGI:6343551).
Structure Level Pattern Image Note
TS15: future midbrain floor plate Present 1Ad
TS15: future midbrain Present Regionally restricted 1Ad Expression in the ventral midbrain overlapped with Foxa2 to a large extent, except for a group of cells near the alar/basal boundary that only expresses Foxa2.
Specimen 1Af: embryonic day 10.5 (more )
Note: Double labeled: red - Foxa1; green - Foxa2 (assay MGI:6343551).
Structure Level Pattern Image Note
TS17: midbrain floor plate Present 1Af
TS17: midbrain Present Regionally restricted 1Af Expression in the ventral midbrain overlapped with Foxa2 to a large extent, except for a group of cells near the alar/basal boundary that only expresses Foxa2.
Specimen 1Ah: embryonic day 11.5 (more )
Note: Double labeled: red - Foxa1; green - Foxa2 (assay MGI:6343551).
Structure Level Pattern Image Note
TS19: midbrain floor plate Present 1Ah
TS19: midbrain Present Regionally restricted 1Ah Expression in the ventral midbrain overlapped with Foxa2 to a large extent, except for a group of cells near the alar/basal boundary that only expresses Foxa2.
Specimen 1Aj: embryonic day 12.5 (more )
Note: Double labeled: red - Foxa1; green - Foxa2 (assay MGI:6343551).
Structure Level Pattern Image Note
TS20: midbrain floor plate Present 1Aj
TS20: midbrain Present Regionally restricted 1Aj Expression in the ventral midbrain overlapped with Foxa2 to a large extent, except for a group of cells near the alar/basal boundary that only expresses Foxa2.
Specimen 2b: embryonic day 8.5; Foxa2tm1Khk/Foxa2tm1Khk (more )
Note: Control. Double labeled: red - Foxa1; green - Foxa2 (assay MGI:6343551).
Structure Level Pattern Image Note
TS13: future midbrain Present Regionally restricted 2b Expression was detected in ventral midbrain.
Specimen 2d: embryonic day 8.5; Foxa2tm1Khk/Foxa2tm1Khk, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant. Double labeled: red - Foxa1; green - Foxa2 (assay MGI:6343551).
Structure Level Pattern Image Note
TS13: future midbrain floor plate Present Regionally restricted 2d Expression was severely reduced in the conditional knockout embryos. Expression was detected only in residual Foxa2-positive cells in the medial floor plate.
Specimen 2f: embryonic day 9.5; Foxa2tm1Khk/Foxa2tm1Khk (more )
Note: Control. Double labeled: red - Foxa1; green - Foxa2 (assay MGI:6343551).
Structure Level Pattern Image Note
TS15: future midbrain Present Regionally restricted 2f Expression was detected in ventral midbrain.
Specimen 2h: embryonic day 9.5; Foxa2tm1Khk/Foxa2tm1Khk, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant. Double labeled: red - Foxa1; green - Foxa2 (assay MGI:6343551).
Structure Level Pattern Image Note
TS15: future midbrain floor plate Present Regionally restricted 2h Expression was severely reduced in the conditional knockout embryos. Expression was detected only in residual Foxa2-positive cells in the medial floor plate.
Specimen 2j: embryonic day 10.5; Foxa2tm1Khk/Foxa2tm1Khk (more )
Note: Control. Double labeled: red - Foxa1; green - Foxa2 (assay MGI:6343551).
Structure Level Pattern Image Note
TS17: midbrain Present Regionally restricted 2j Expression was detected in ventral midbrain.
Specimen 2l: embryonic day 10.5; Foxa2tm1Khk/Foxa2tm1Khk, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant. posterior midbrain. Double labeled: red - Foxa1; green - Foxa2 (assay MGI:6343551).
Structure Level Pattern Image Note
TS17: midbrain floor plate Present Regionally restricted 2l In the posterior midbrain, expression was severely reduced in the conditional knockout embryos. Expression was detected only in residual Foxa2-positive cells in the medial floor plate.
Specimen 2n: embryonic day 10.5; Foxa2tm1Khk/Foxa2tm1Khk, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant. anterior midbrain. Double labeled: red - Foxa1; green - Foxa2 (assay MGI:6343551).
Structure Level Pattern Image Note
TS17: midbrain Present Regionally restricted 2n In the anterior midbrain, expression was similar to control.
Specimen 2p: embryonic day 12.5; Foxa1tm1Gsc/Foxa1tm1Gsc, Foxa2tm1Khk/Foxa2+ (more )
Note: Control; genotype either Foxa fl/fl; Foxa1 +/+ or Foxa2 fl/fl; Foxa1 +/-. Double labeled: red - Foxa1; green - Foxa2 (assay MGI:6343551).
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted 2p Expression was detected in ventral midbrain.
Specimen 2r: embryonic day 12.5; Foxa2tm1Khk/Foxa2tm1Khk, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant. Double labeled: red - Foxa1; green - Foxa2 (assay MGI:6343551).
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted 2r At E12.5, expression in the conditional knockout was similar to control in the entire midbrain.
Specimen 2t: embryonic day 12.5; Foxa1tm1Gsc/Foxa1tm1Gsc (more )
Note: Double labeled: red - Foxa1; green - Foxa2 (assay MGI:6343551).
Structure Level Pattern Image Note
TS20: midbrain Absent 2t
Specimen 2v: embryonic day 12.5; Foxa1tm1Gsc/Foxa1tm1Gsc, Foxa2tm1Khk/Foxa2tm1Khk (more )
Note: Conditional mutant. Double labeled: red - Foxa1; green - Foxa2 (assay MGI:6343551).
Structure Level Pattern Image Note
TS20: midbrain Absent 2v
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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06/12/2024
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