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Gene Expression Data
Assay Details
Assay
Reference: J:187741 Hu JK, et al., Autonomous and nonautonomous roles of Hedgehog signaling in regulating limb muscle formation. Genes Dev. 2012 Sep 15;26(18):2088-102
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6358478
Gene symbol: Pax3
Gene name: paired box 3
Antibody: Anti-Pax3 (clone 274212, MAP2457)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 3E: embryonic day 10.5; Smotm1Amc/Smo+, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Double labeled: green - Pax3; red - Myf5 (assay MGI:6358479).
Structure Level Pattern Image Note
TS17: forelimb bud Present Regionally restricted 3E Expression was detected in muscle progenitor cells.
Specimen 3F: embryonic day 10.5; Smotm1Amc/Smotm2Amc, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del. Double labeled: green - Pax3; red - Myf5 (assay MGI:6358479).
Structure Level Pattern Image Note
TS17: forelimb bud Present Regionally restricted 3F Expression in conditional knockout muscle progenitor cells was comparable to wild type.
Specimen 3K: embryonic day 10.75; Smotm1Amc/Smo+, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Double labeled: green - Pax3; red - Myf5 (assay MGI:6358479).
Structure Level Pattern Image Note
TS17: forelimb bud Present Regionally restricted 3K Expression was detected in muscle progenitor cells.
Specimen 3L: embryonic day 10.75; Smotm1Amc/Smotm2Amc, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del. Double labeled: green - Pax3; red - Myf5 (assay MGI:6358479).
Structure Level Pattern Image Note
TS17: forelimb bud Present Regionally restricted 3L Expression in conditional knockout muscle progenitor cells was comparable to wild type.
Specimen 5A: embryonic day 10.5; Smotm2Amc/Smo+, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Conditional mutant. Double labeled: green - Pax3; red - Casp3 (assay MGI:6358503).
Structure Level Pattern Image Note
TS17: dermomyotome Present Regionally restricted 5A Expression was detected in muscle progenitor cells.
TS17: limb bud Present Regionally restricted 5A Expression was detected in muscle progenitor cells.
Specimen 5B: embryonic day 10.5; Smotm2Amc/Smotm2Amc, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del. Double labeled: green - Pax3; red - Casp3 (assay MGI:6358503).
Structure Level Pattern Image Note
TS17: limb bud Present Regionally restricted 5B Expression was detected in muscle progenitor cells. No increased apoptosis was observed in muscle cells migrated into the limb bud.
TS17: dermomyotome Present Regionally restricted 5B Expression was detected in muscle progenitor cells. In the dermomyotome, increased apoptosis was noted in the muscle progenitor cells in the ventral lateral lip.
Specimen 5H: embryonic day 10.5; Smotm2Amc/Smotm2Amc, Myf5tm3(cre)Sor/Myf5+ (more )
Note: Conditional mutant. Double labeled: green - Pax3; red - Casp3 (assay MGI:6358503).
Structure Level Pattern Image Note
TS17: dermomyotome Present Regionally restricted 5H Expression was detected in muscle progenitor cells with no evidence of ectopic or increased cell death.
Specimen S1C: embryonic day 10.25; Smotm2Amc/Smo+, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS16: dermomyotome Present S1C
Specimen S1D: embryonic day 10.25; Smotm2Amc/Smotm2Amc, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
Structure Level Pattern Image Note
TS16: dermomyotome Present S1D
Specimen S4E: embryonic day 10.75; Smotm1Amc/Smo+, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Double labeled: green - Pax3; red - Myf5 (assay MGI:6358479).
Structure Level Pattern Image Note
TS17: hindlimb bud Present Regionally restricted S4E Expression was detected in muscle progenitor cells.
Specimen S4F: embryonic day 10.75; Smotm1Amc/Smotm2Amc, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del. Double labeled: green - Pax3; red - Myf5 (assay MGI:6358479).
Structure Level Pattern Image Note
TS17: hindlimb bud Present Regionally restricted S4F Expression in conditional knockout muscle progenitor cells was comparable to wild type.
Specimen S4K: embryonic day 11.0; Smotm1Amc/Smo+, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Double labeled: green - Pax3; red - Myf5 (assay MGI:6358479).
Structure Level Pattern Image Note
TS18: hindlimb bud Present Regionally restricted S4K Expression was detected in muscle progenitor cells.
Specimen S4L: embryonic day 11.0; Smotm1Amc/Smotm2Amc, Pax3tm1(cre)Joe/Pax3+ (more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del. Double labeled: green - Pax3; red - Myf5 (assay MGI:6358479).
Structure Level Pattern Image Note
TS18: hindlimb bud Present Regionally restricted S4L Expression in conditional knockout muscle progenitor cells was comparable to wild type.
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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09/17/2024
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