Gene Expression Data
Assay Details
Assay
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Reference:
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J:147561
Blatt EN, et al., Forkhead transcription factor HFH-4 expression is temporally related to ciliogenesis. Am J Respir Cell Mol Biol. 1999 Aug;21(2):168-76
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6376476
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Gene symbol:
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Foxj1
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Gene name:
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forkhead box J1
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Antibody:
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anti-HNF-4
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
2A:
postnatal adult
(more )
Specimen
2A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
10% Formalin
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: lung epithelium |
Present |
Regionally restricted |
2A
|
Expression was detected in a subpopulation of proximal airway epithelial cells.
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Specimen
2D:
postnatal adult
(more )
Specimen
2D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
10% Formalin
|
Embedding:
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Paraffin
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Specimen
2F:
postnatal adult
(more )
Note: Double labeled: brown - Foxj1; blue - Scgb1a1 (assay MGI:6376482).
Specimen
2F:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
10% Formalin
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: brown - Foxj1; blue - Scgb1a1 (assay MGI:6376482).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: lung epithelium |
Present |
Regionally restricted |
2F
|
Double immunostaining revealed that expression of Foxj1 and Scgb1a1 in the airway are in two mutually exclusive cell populations.
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Specimen
3A:
postnatal adult
(more )
Note: Double labeled: brown - Foxj1; blue - Omp (assay MGI:6376483).
Specimen
3A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
10% Formalin
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: brown - Foxj1; blue - Omp (assay MGI:6376483).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: nasal epithelium |
Present |
Regionally restricted |
3A
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Expression was detected in ciliated cells in a predominant nuclear pattern.
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TS28: paranasal sinus |
Present |
Regionally restricted |
3A
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Expression was detected in ciliated cells in a predominant nuclear pattern.
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Specimen
3B:
postnatal adult
(more )
Specimen
3B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
10% Formalin
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: nasal epithelium |
Present |
Regionally restricted |
3B
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Expression was detected in ciliated cells in a predominant nuclear pattern.
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Specimen
3E:
embryonic day 15.5
(more )
Specimen
3E:
(close )
Genetic Background:
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Not Specified
|
Age:
|
embryonic day 15.5
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Sex:
|
Not Specified
|
Type:
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section
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Fixation:
|
10% Formalin
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: nasal epithelium |
Present |
Regionally restricted |
3E
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Expression was detected in nonciliated cells.
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Specimen
4A:
postnatal adult
(more )
Specimen
4A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
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Sex:
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Female
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Type:
|
section
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Fixation:
|
10% Formalin
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Embedding:
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Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: epithelial layer of oviduct |
Strong |
Regionally restricted |
4A
,
4B
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High expression was detected in proximal oviduct within almost every ciliated epithelial cell lining the lumen. There are fewer ciliated cells in distal oviduct, and in these regions expression is found in fewer cells. Secretory, nonciliated cells do not show expression.
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Specimen
4D:
embryonic day 15.5
(more )
Specimen
4D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
10% Formalin
|
Embedding:
|
Paraffin
|
Specimen
4E:
postnatal adult
(more )
Specimen
4E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
10% Formalin
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Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: brain ependyma |
Present |
Regionally restricted |
4E
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Expression was detected in the ependymal cells lining the ventricles of the brain in a predominantly nuclear pattern.
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Specimen
not shown:
postnatal adult
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
10% Formalin
|
Embedding:
|
Paraffin
|
Specimen
5A:
embryonic day 14.5
(more )
Specimen
5A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
10% Formalin
|
Embedding:
|
Paraffin
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Specimen
5C:
embryonic day 15.5
(more )
Specimen
5C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
10% Formalin
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: lung epithelium |
Present |
Regionally restricted |
5C
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Expression was detected in approximately half of the proximal airway epithelial cells at E15.5.
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Specimen
5E:
postnatal newborn
(more )
Specimen
5E:
(close )
Genetic Background:
|
Not Specified
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Age:
|
postnatal newborn
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
10% Formalin
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Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: lung epithelium |
Present |
Regionally restricted |
5E
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At birth, expression was detected throughout the proximal airway in a pattern similar to the adult lung.
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Specimen
6A:
postnatal adult
(more )
Specimen
6A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Male
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Type:
|
section
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Fixation:
|
10% Formalin
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Embedding:
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Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spermatocyte |
Weak |
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6A
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Weak expression was detected in the cytoplasm of secondary spermatocytes.
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TS28: spermatid |
Absent |
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6A
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No expression was detected in spermatids (stage I) during early maturation.
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Specimen
6B:
postnatal adult
(more )
Specimen
6B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
10% Formalin
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spermatid |
Strong |
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6B
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Abundant expression was detected in the nucleus in spermatids at stages III to IV.
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Specimen
6C:
postnatal adult
(more )
Specimen
6C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
10% Formalin
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spermatid |
Strong |
|
6C
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Abundant expression was detected in the nucleus in spermatids at stages V to VI.
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Specimen
6D:
postnatal adult
(more )
Specimen
6D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
10% Formalin
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spermatid |
Absent |
|
6D
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After stage VII, expression was extinguished in spermatids while development of the flagella continued.
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TS28: spermatocyte |
Present |
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6D
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Expression was detected in the cytoplasm of spermatocytes at stages VII to VIII.
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Specimen
6E:
postnatal adult
(more )
Specimen
6E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
10% Formalin
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spermatid |
Absent |
|
6E
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After stage VII, expression was extinguished in spermatids while development of the flagella continued.
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TS28: spermatocyte |
Weak |
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6E
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Weak expression was detected in spermatocytes at stages XI through XIII.
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