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Gene Expression Data
Assay Details
Assay
Reference: J:275243 Bosch PJ, et al., A critical role for the nuclear protein Akirin2 in the formation of mammalian muscle in vivo. Genesis. 2019 May;57(5):e23286
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6379284
Gene symbol: Casp3
Gene name: caspase 3
Antibody: anti-cleaved Caspase-3 (Asp175; #9661)
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: The antibody recognized the cleaved form of the protein.
Results
Specimen 5a Sim1-Cre;Aki2fl/+: embryonic day 10.5; Akirin2tm1Aki/Akirin2+, Tg(Sim1-cre)1Lowl/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: somite Absent 5a Sim1-Cre;Aki2fl/+ , 5b Sim1-Cre;Aki2fl/+
TS17: future spinal cord Present 5b Sim1-Cre;Aki2fl/+ , 5a Sim1-Cre;Aki2fl/+
Specimen 5a Sim1-Cre;Aki2fl/fl: embryonic day 10.5; Akirin2tm1Aki/Akirin2tm1Aki, Tg(Sim1-cre)1Lowl/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: somite Strong 5a Sim1-Cre;Aki2fl/fl , 5b Sim1-Cre;Aki2fl/fl
TS17: future spinal cord Present 5b Sim1-Cre;Aki2fl/fl , 5a Sim1-Cre;Aki2fl/fl Normal staining was observed.
Specimen 5c Sim1-Cre;Aki2fl/+: embryonic day 10.5; Akirin2tm1Aki/Akirin2+, Tg(Sim1-cre)1Lowl/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: forelimb bud Absent 5c CC3 Sim1-Cre;Aki2fl/+
TS17: trunk Absent 5c CC3 Sim1-Cre;Aki2fl/+
Specimen 5c Sim1-Cre;Aki2fl/fl: embryonic day 10.5; Akirin2tm1Aki/Akirin2tm1Aki, Tg(Sim1-cre)1Lowl/0 (more )
Note: Conditional mutant. Double labeled: red - Casp3; green - Pax3 (assay MGI:6379244).
Structure Level Pattern Image Note
TS17: trunk Present Regionally restricted 5c CC3 Sim1-Cre;Aki2fl/fl , 5c Inset Merge Sim1-Cre;Aki2fl/fl A small population of Pax3-positive cells were found at the border to the limb bud, and some of them were Casp3-positive.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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09/17/2024
MGI 6.24 		The Jackson Laboratory