Pecam1 Immunohistochemistry Gene Expression Assay - GXD

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Gene Expression Data
Assay Details

Assay

Reference:	J:137692 Huang X, et al., Coronary development is regulated by ATP-dependent SWI/SNF chromatin remodeling component BAF180. Dev Biol. 2008 Jul 15;319(2):258-66
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6387272
Gene symbol:	Pecam1
Gene name:	platelet/endothelial cell adhesion molecule 1

Antibody:	anti-PECAM
Detection system:	Alexa Fluor 488

Results

5L ©

Specimen 5L: embryonic day 14.5; Pbrm1^tm1Rtj/Pbrm1^tm1Rtj (more

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Note: Triple labeled: green - Pecam1; red - Acta2 (assay MGI:6387271); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS22: heart endothelium	Present	Regionally restricted	5L	Authors report little penetration into myocardium of mutant heart.

5M ©

Specimen 5M: embryonic day 14.5 (more

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Note: Triple labeled: green - Pecam1; red - Acta2 (assay MGI:6387271); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS22: coronary artery	Present	Regionally restricted	5M	Expression in coronary vessels penetrating into myocardium.

S2_-/- (upper) ©

Specimen S2 -/- (upper): embryonic day 14.5; Pbrm1^tm1Rtj/Pbrm1^tm1Rtj (more

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Note: Triple labeled: green - Pecam1; red - Acta2 (assay MGI:6387271); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS22: ascending aorta	Present		S2_-/- (upper)	Authors report mutant embryos form a normal connection between coronary vessels and the aorta.
TS22: coronary artery	Present		S2_-/- (upper)	Authors report mutant embryos form a normal connection between coronary vessels and the aorta.

S2_-/- (middle) ©

Specimen S2 -/- (middle): embryonic day 14.5; Pbrm1^tm1Rtj/Pbrm1^tm1Rtj (more

)
Note: Triple labeled: green - Pecam1; red - Acta2 (assay MGI:6387271); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS22: ascending aorta	Present		S2_-/- (middle)	Authors report mutant embryos form a normal connection between coronary vessels and the aorta.
TS22: coronary artery	Present		S2_-/- (middle)	Authors report mutant embryos form a normal connection between coronary vessels and the aorta.

S2_-/- (lower) ©

Specimen S2 -/- (lower): embryonic day 14.5; Pbrm1^tm1Rtj/Pbrm1^tm1Rtj (more

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Note: Triple labeled: green - Pecam1; red - Acta2 (assay MGI:6387271); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS22: coronary artery	Present		S2_-/- (lower)

S2_+/+ (upper) ©

Specimen S2 +/+ (upper): embryonic day 14.5 (more

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Note: Triple labeled: green - Pecam1; red - Acta2 (assay MGI:6387271); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS22: ascending aorta	Present		S2_+/+ (upper)
TS22: coronary artery	Present		S2_+/+ (upper)

S2_+/+ (middle) ©

Specimen S2 +/+ (middle): embryonic day 14.5 (more

)
Note: Triple labeled: green - Pecam1; red - Acta2 (assay MGI:6387271); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS22: ascending aorta	Present		S2_+/+ (middle)
TS22: coronary artery	Present		S2_+/+ (middle)

S2_+/+ (lower) ©

Specimen S2 +/+ (lower): embryonic day 14.5 (more

)
Note: Triple labeled: green - Pecam1; red - Acta2 (assay MGI:6387271); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS22: coronary artery	Present		S2_+/+ (lower)

6B -/- ©

Specimen 6B -/-: embryonic day 13.5; Pbrm1^tm1Rtj/Pbrm1^tm1Rtj (more

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Note: Triple labeled: green - Pecam1; red - Acta2 (assay MGI:6387271); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS21: ascending aorta	Present		6B -/-
TS21: pulmonary trunk	Present		6B -/-

6B +/+ ©

Specimen 6B +/+: embryonic day 13.5 (more

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Note: Triple labeled: green - Pecam1; red - Acta2 (assay MGI:6387271); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS21: ascending aorta	Present		6B +/+
TS21: pulmonary trunk	Present		6B +/+

6C -/- (upper) ©

Specimen 6C -/- (upper): embryonic day 13.5; Pbrm1^tm1Rtj/Pbrm1^tm1Rtj (more

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Note: Triple labeled: green - Pecam1; red - Acta2 (assay MGI:6387271); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS21: heart endothelium	Present		6C -/- (upper)

6C -/- (lower) ©

Specimen 6C -/- (lower): embryonic day 13.5; Pbrm1^tm1Rtj/Pbrm1^tm1Rtj (more

)
Note: Triple labeled: green - Pecam1; red - Acta2 (assay MGI:6387271); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS21: heart endothelium	Present	Regionally restricted	6C -/- (lower)	Expression in disorganized endothelial cells inside nodules.

6C +/- (upper) ©

Specimen 6C +/- (upper): embryonic day 13.5; Pbrm1^tm1Rtj/Pbrm1⁺ (more

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Note: Triple labeled: green - Pecam1; red - Acta2 (assay MGI:6387271); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS21: blood vessel endothelium	Present		6C +/- (upper)

6C +/- (lower) ©

Specimen 6C +/- (lower): embryonic day 13.5; Pbrm1^tm1Rtj/Pbrm1⁺ (more

)
Note: Triple labeled: green - Pecam1; red - Acta2 (assay MGI:6387271); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS21: blood vessel endothelium	Present		6C +/- (lower)

6D -/- ©

Specimen 6D -/-: embryonic day 14.5; Pbrm1^tm1Rtj/Pbrm1^tm1Rtj (more

)
Note: Triple labeled: green - Pecam1; red - Acta2 (assay MGI:6387271); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS22: heart endothelium	Present	Regionally restricted	6D -/-	Expression in large number of endothelial cells inside larger nodules.

6D +/- ©

Specimen 6D +/-: embryonic day 14.5; Pbrm1^tm1Rtj/Pbrm1⁺ (more

)
Note: Triple labeled: green - Pecam1; red - Acta2 (assay MGI:6387271); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS22: blood vessel endothelium	Present	Regionally restricted	6D +/-

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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