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Gene Expression Data
Assay Details
Assay
Reference: J:266583 Baumgartner M, et al., Minor spliceosome inactivation causes microcephaly, owing to cell cycle defects and death of self-amplifying radial glial cells. Development. 2018 Aug 28;145(17):dev166322
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6400724
Gene symbol: Rbfox3
Gene name: RNA binding protein, fox-1 homolog (C. elegans) 3
Results
Specimen 3A control: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: magenta - Rbfox3; green - Mki67 (assay MGI:6400719).
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted 3A control Expression was detected in neurons.
Specimen 3A mutant: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: magenta - Rbfox3; green - Mki67 (assay MGI:6400719).
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted 3A mutant The number of immunopositive neurons was not changed in the E12 mutant pallium.
Specimen 3B control: embryonic day 13.5; Rnu11tm1.1Rank/Rnu11+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: magenta - Rbfox3; green - Mki67 (assay MGI:6400719).
Structure Level Pattern Image Note
TS21: neocortex Present Regionally restricted 3B control Expression was detected in neurons.
Specimen 3B mutant: embryonic day 13.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: magenta - Rbfox3; green - Mki67 (assay MGI:6400719).
Structure Level Pattern Image Note
TS21: neocortex Present Regionally restricted 3B mutant The number of immunopositive neurons was not changed in the E13 mutant pallium.
Specimen 3C control: embryonic day 14.5; Rnu11tm1.1Rank/Rnu11+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: magenta - Rbfox3; green - Mki67 (assay MGI:6400719).
Structure Level Pattern Image Note
TS22: neocortex Present Regionally restricted 3C control Expression was detected in neurons.
Specimen 3C mutant: embryonic day 14.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: magenta - Rbfox3; green - Mki67 (assay MGI:6400719).
Structure Level Pattern Image Note
TS22: neocortex Present Regionally restricted 3C mutant At E14 the number of immunopositive neurons was significantly reduced in the mutant pallium.
Specimen 4F right: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: green - Rbfox3; magenta - H2ax (assay MGI:6400789).
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted 4F NeuN Expression was detected in neurons in the mutant pallium. There was virtually no co-expression with H2ax (5.1%).
Specimen S2A E12 control: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Triple labeled: magenta - Rbfox3; green - Mki67 (assay MGI:6400719); white - Rnu11 (assay MGI:6400707).
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted S2A E12 ctrl top , S2A E12 ctrl bottom Expression was detected in neurons.
Specimen S2A E12 mutant: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Triple labeled: magenta - Rbfox3; green - Mki67 (assay MGI:6400719); white - Rnu11 (assay MGI:6400707).
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted S2A E12 mut bottom , S2A E12 mut top Rnu11 FISH in conjunction with immunolabeling showed the presence of Rnu11-null neural progenitor cells (Mki67+) and Rnu11-null neurons (Rbfox3+) in the mutant pallium.
Specimen S2A E13 control: embryonic day 13.5; Rnu11tm1.1Rank/Rnu11+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Triple labeled: magenta - Rbfox3; green - Mki67 (assay MGI:6400719); white - Rnu11 (assay MGI:6400707).
Structure Level Pattern Image Note
TS21: neocortex Present Regionally restricted S2A E13 ctrl bottom , S2A E13 ctrl top Expression was detected in neurons.
Specimen S2A E13 mutant: embryonic day 13.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Triple labeled: magenta - Rbfox3; green - Mki67 (assay MGI:6400719); white - Rnu11 (assay MGI:6400707).
Structure Level Pattern Image Note
TS21: neocortex Present Regionally restricted S2A E13 mut bottom , S2A E13 mut top Rnu11 FISH in conjunction with immunolabeling showed the presence of Rnu11-null neural progenitor cells (Mki67+) and Rnu11-null neurons (Rbfox3+) in the mutant pallium.
Specimen S2F control: embryonic day 13.5; Rnu11tm1.1Rank/Rnu11+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: green - Rbfox3; magenta - BrdU.
Structure Level Pattern Image Note
TS21: neocortex Present Regionally restricted S2F ctrl Expression was detected in neurons.
Specimen S2F mutant: embryonic day 13.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: green - Rbfox3; magenta - BrdU.
Structure Level Pattern Image Note
TS21: neocortex Present Regionally restricted S2F mut There was a signficant decrease in Rbfox3+/BrdU+ cells as a percentage of all Rbfox3-positive cells in the E13 mutant pallium compared with the control.
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/09/2024
MGI 6.24 		The Jackson Laboratory