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Gene Expression Data
Assay Details
Assay
Reference: J:224632 Lowe KL, et al., Podoplanin and CLEC-2 drive cerebrovascular patterning and integrity during development. Blood. 2015 Jun 11;125(24):3769-77
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6407082
Gene symbol: Pdpn
Gene name: podoplanin
Antibody: Anti-Podoplanin (clone 8.1.1)
Detection system: Secondary antibody coupled to Cy3
Results
Specimen 1A E11.5: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: neural tube Present Widespread 1A top left , 1A top right Widely expressed throughout the neural tube on neuro-epithelial cells.
Specimen 1A E14.5: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: choroid plexus Present 1A bottom right , 1A bottom left
TS22: brain ventricle Present Regionally restricted 1A bottom right , 1A bottom left Expressed in the ependymal lining.
Specimen S2A: embryonic day 11.5 (more )
Note: Double labeled: red - Pdpn; green - Nes (assay MGI:6407114).
Structure Level Pattern Image Note
TS19: neural tube Present Widespread S2A Pdpn , S2A merge Widely expressed along with the intermediate filament protein, Nestin, throughout the neural tube on neuro-epithelial cells.
Specimen S2B control: embryonic day 11.5; Pdpntm1Arte/Pdpntm1Arte (more )
Note: Double labeled: red - Pdpn; green - Nes (assay MGI:6407114).
Structure Level Pattern Image Note
TS19: neural tube Present Widespread S2B fl/fl Widely expressed along with the intermediate filament protein, Nestin, throughout the neural tube on neuro-epithelial cells.
Specimen S2B cKO: embryonic day 11.5; Pdpntm1Arte/Pdpntm1Arte, Tg(Nes-cre)1Wmz/0 (more )
Note: Conditional mutant. Double labeled: red - Pdpn; green - Nes (assay MGI:6407114).
Structure Level Pattern Image Note
TS19: neural tube Absent S2B cKO
Specimen 4A +/+: embryonic day 11.5 (more )
Note: Triple labeled: red - Pdpn; green - Pecam1 (assay MGI:6407096); blue - Cspg4 (assay MGI:6407113).
Structure Level Pattern Image Note
TS19: neural tube Present 4A +/+ merge , 4A +/+ Pdpn
Specimen 4A Clec-2-/-: embryonic day 11.5; Clec1btm1.2Arte/Clec1btm1.2Arte (more )
Note: Triple labeled: red - Pdpn; green - Pecam1 (assay MGI:6407096); blue - Cspg4 (assay MGI:6407113).
Structure Level Pattern Image Note
TS19: neural tube Present 4A Clec-2-/- Pdpn , 4A Clec-2-/- merge
Specimen 4A Pdpn PGK-cKO: embryonic day 11.5; Pdpntm1Arte/Pdpntm1Arte, Tg(Pgk1-cre)1Lni/0 (more )
Note: Conditional mutant. Triple labeled: red - Pdpn; green - Pecam1 (assay MGI:6407096); blue - Cspg4 (assay MGI:6407113).
Structure Level Pattern Image Note
TS19: neural tube Absent 4A Pdpn PGK cKO Pdpn , 4A Pdpn PGK cKO merge
Specimen 4A Pdpn Nes-cKO: embryonic day 11.5; Pdpntm1Arte/Pdpntm1Arte, Tg(Nes-cre)1Wmz/0 (more )
Note: Conditional mutant. Triple labeled: red - Pdpn; green - Pecam1 (assay MGI:6407096); blue - Cspg4 (assay MGI:6407113).
Structure Level Pattern Image Note
TS19: neural tube Absent 4A Pdpn Nes cKO Pdpn , 4A Pdpn Nes cKO merge
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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06/12/2024
MGI 6.13 		The Jackson Laboratory