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Gene Expression Data
Assay Details
Assay
Reference: J:257927 Fairchild CL, et al., RBX2 maintains final retinal cell position in a DAB1-dependent and -independent fashion. Development. 2018 Feb 2;145(3):dev155283
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6430451
Gene symbol: Otx2
Gene name: orthodenticle homeobox 2
Results
Specimen 4A': postnatal day 20 (more )
Note: Double labeled: green - Otx2; red - Prkca (assay MGI:6430465). The genetic background of the conditional control specimen was not specified.
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present Regionally restricted 4A' Wild type rod bipolar cells (Otx2+ and Prkca+) localize at the apical side of the inner nuclear layer. Fewer than 1% of rod bipolar cells were ectopically localized at the inner half of the inner nuclear layer of control retinas.
TS28: retina inner plexiform layer Present Regionally restricted 4A' Rod bipolar cells extend their axons to the inner plexiform layer.
Specimen 4B': postnatal day 20; Rnf7tm1c(EUCOMM)Wtsi/Rnf7tm1c(EUCOMM)Wtsi, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Double labeled: green - Otx2; red - Prkca (assay MGI:6430465).
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present Regionally restricted 4B' In the conditional mutant retinas, more than 10% of rod bipolar cells were ectopically localized at the inner half of the inner nuclear layer.
TS28: retina inner plexiform layer Present 4B'
Specimen 4C': postnatal day 20 (more )
Note: Double labeled: green - Otx2; red - Prkca (assay MGI:6430465). The genetic background of the conditional control specimen was not specified.
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present Regionally restricted 4C' In the controls, rod bipolar cells (Otx2+,Prkca+) have complex dendritic arbors located at the top of the inner nuclear layer.
TS28: retina inner plexiform layer Present Regionally restricted 4C' Rod bipolar cell axons of controls terminate at the outer nuclear sublamina of the inner plexiform layer.
Specimen 4D': postnatal day 20; Rnf7tm1c(EUCOMM)Wtsi/Rnf7tm1c(EUCOMM)Wtsi, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Double labeled: green - Otx2; red - Prkca (assay MGI:6430465).
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present Regionally restricted 4D' The conditional mutant displaced rod bipolar cell dendritic arbors exhibit simpler shorter dendritic protrusions that often appear to be incorrectly polarized.
TS28: retina inner plexiform layer Present Regionally restricted 4D' The axons of the displaced rod bipolar cells do not seem to be affected in the conditional mutant.
Specimen not shown P9 control: postnatal day 9 (more )
Note: Also stained for Prkca (assay MGI:6430465). The genetic background of the conditional control specimen was not specified.
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present Regionally restricted At P9, almost all wild type rod bipolar cells (Otx2+,Prkca+) have reached their final position.
Specimen not shown P9 Rbx2cKO-Nes: postnatal day 9; Rnf7tm1c(EUCOMM)Wtsi/Rnf7tm1c(EUCOMM)Wtsi, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Also stained for Prkca (assay MGI:6430465).
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present Regionally restricted At P9 there was no significant difference between conditional mutants and control rod bipolar cells, with most of the cells in both cases localized at the apical side.
Specimen not shown P15 control: postnatal day 15 (more )
Note: Also stained for Prkca (assay MGI:6430465). The genetic background of the conditional control specimen was not specified.
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present Regionally restricted Expression was detected in rod bipolar cells (Otx2+,Prkca+) localized at the apical side of the inner nuclear layer.
Specimen not shown P15 Rbx2cKO-Nes: postnatal day 15; Rnf7tm1c(EUCOMM)Wtsi/Rnf7tm1c(EUCOMM)Wtsi, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Also stained for Prkca (assay MGI:6430465).
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present Regionally restricted At later time points, an increasing number of misplaced rod bipolar cells were observed. In the conditional mutant retinas, the ectopic localization occurs after their normal migration period.
Specimen not shown P21 control: postnatal day 21 (more )
Note: Also stained for Prkca (assay MGI:6430465). The genetic background of the conditional control specimen was not specified.
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present Regionally restricted Expression was detected in rod bipolar cells (Otx2+,Prkca+) localized at the apical side of the inner nuclear layer.
Specimen not shown P21 Rbx2cKO-Nes: postnatal day 21; Rnf7tm1c(EUCOMM)Wtsi/Rnf7tm1c(EUCOMM)Wtsi, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Also stained for Prkca (assay MGI:6430465).
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present Regionally restricted At later time points, an increasing number of misplaced rod bipolar cells were observed. In the conditional mutant retinas, the ectopic localization occurs after their normal migration period.
Specimen not shown P21 Rbx2cKO-Nes;Dab1+/-: postnatal day 21; Dab1tm1Cpr/Dab1+, Rnf7tm1c(EUCOMM)Wtsi/Rnf7tm1c(EUCOMM)Wtsi, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Also stained for Prkca (assay MGI:6430465).
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present Regionally restricted Removing one copy of Dab1 rescued rod bipolar cell displacement in the conditional mutant.
Specimen 5D: postnatal day 20; Rnf7tm1c(EUCOMM)Wtsi/Rnf7tm1c(EUCOMM)Wtsi, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Double labeled: red - Otx2; green - Arr3 (assay MGI:6430500).
Structure Level Pattern Image Note
TS28: retina layer Present Regionally restricted 5D' , 5D In the conditional mutant, the identity of ectopic cone photoreceptors was confirmed by co-localization of Otx2 and Arr3.
Specimen not shown SOCS7 Wt: postnatal day 20 (more )
Note: Also stained for Prkca (assay MGI:6430465).
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present Regionally restricted A low percentage of wild type rod bipolar cells (Otx2+ and Prkca+) were detected in the inner half of the inner nuclear layer.
Specimen not shown SOCS7 Mut: postnatal day 20; Socs7tm1b(EUCOMM)Wtsi/Socs7tm1b(EUCOMM)Wtsi (more )
Note: Also stained for Prkca (assay MGI:6430465).
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present Regionally restricted In the knockout retinas, a higher percentage of rod bipolar cells were ectopically localized at the inner half of the inner nuclear layer.
Specimen S3G': postnatal day 20 (more )
Note: Double labeled: green - Otx2; red - Prkca (assay MGI:6430465). The genetic background of the conditional control specimen was not specified.
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present Regionally restricted S3G' Rod bipolar cells (Otx2+,Prkca+) were localized at the apical side of the inner nuclear layer.
Specimen S3H': postnatal day 20; Rnf7tm1c(EUCOMM)Wtsi/Rnf7tm1c(EUCOMM)Wtsi, Tg(Crx-cre)352Gla/0 (more )
Note: Conditional mutant. Double labeled: green - Otx2; red - Prkca (assay MGI:6430465).
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present Regionally restricted S3H' Normal localization of rod bipolar cells was seen in Rbx2cKO-Crx retinas.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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11/05/2024
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