Gene Expression Data
Assay Details
Assay
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Reference:
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J:210985
Wolfe AD, et al., Mixl1 localizes to putative axial stem cell reservoirs and their posterior descendants in the mouse embryo. Gene Expr Patterns. 2014 May;15(1):8-20
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:6436904
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Gene symbol:
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Runx1
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Gene name:
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runt related transcription factor 1
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Reporter:
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lacZ
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Direct Detection
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Results
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Specimen
6A:
embryonic day 7.5; Runx1tm2Spe/Runx1 +
(more )
Note: Early headfold stage. Double labeled: blue - Runx1 reporter; brown - Mixl1 (assay MGI:6436901).
Specimen
6A:
(close )
Genetic Background:
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involves: 129S4/SvJae * C57BL/6J * CBA
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Mutant Allele(s):
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Runx1tm2Spe/Runx1+
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Age:
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embryonic day 7.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Early headfold stage. Double labeled: blue - Runx1 reporter; brown - Mixl1 (assay MGI:6436901).
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: allantois |
Present |
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6A
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Expression throughout allantois and visceral yolk sac endoderm.
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TS11: yolk sac endoderm |
Present |
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6A
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Expression throughout allantois and visceral yolk sac endoderm.
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Specimen
6B:
embryonic day 7.5; Runx1tm2Spe/Runx1 +
(more )
Note: Early headfold stage. Double labeled: blue - Runx1 reporter; brown - Mixl1 (assay MGI:6436901).
Specimen
6B:
(close )
Genetic Background:
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involves: 129S4/SvJae * C57BL/6J * CBA
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Mutant Allele(s):
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Runx1tm2Spe/Runx1+
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Age:
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embryonic day 7.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Early headfold stage. Double labeled: blue - Runx1 reporter; brown - Mixl1 (assay MGI:6436901).
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: allantois |
Strong |
Regionally restricted |
6B
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Prominent expression throughout distal allantois, in both core and mesothelium.
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Specimen
6C:
embryonic day 7.5; Runx1tm2Spe/Runx1 +
(more )
Note: Early headfold stage. Double labeled: blue - Runx1 reporter; brown - Mixl1 (assay MGI:6436901).
Specimen
6C:
(close )
Genetic Background:
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involves: 129S4/SvJae * C57BL/6J * CBA
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Mutant Allele(s):
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Runx1tm2Spe/Runx1+
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Age:
|
embryonic day 7.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Early headfold stage. Double labeled: blue - Runx1 reporter; brown - Mixl1 (assay MGI:6436901).
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Specimen
6D:
embryonic day 8.0; Runx1tm2Spe/Runx1 +
(more )
Note: 6 somite stage. Double labeled: blue - Runx1 reporter; brown - Mixl1 (assay MGI:6436901).
Specimen
6D:
(close )
Genetic Background:
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involves: 129S4/SvJae * C57BL/6J * CBA
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Mutant Allele(s):
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Runx1tm2Spe/Runx1+
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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6 somite stage. Double labeled: blue - Runx1 reporter; brown - Mixl1 (assay MGI:6436901).
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: allantois |
Present |
Regionally restricted |
6D
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Expression co-localizing with Runx1 within proximal allantois.
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Specimen
6E:
embryonic day 8.0; Runx1tm2Spe/Runx1 +
(more )
Note: 6 somite stage. Double labeled: blue - Runx1 reporter; brown - Mixl1 (assay MGI:6436901).
Specimen
6E:
(close )
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6J * CBA
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Mutant Allele(s):
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Runx1tm2Spe/Runx1+
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Age:
|
embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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6 somite stage. Double labeled: blue - Runx1 reporter; brown - Mixl1 (assay MGI:6436901).
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Specimen
6 somite stage not shown:
embryonic day 8.0; Runx1tm2Spe/Runx1 +
(more )
Note: 6 somite stage.
Specimen
6 somite stage not shown:
(close )
Genetic Background:
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involves: 129S4/SvJae * C57BL/6J * CBA
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Mutant Allele(s):
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Runx1tm2Spe/Runx1+
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Age:
|
embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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6 somite stage.
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Structure |
Level |
Pattern |
Image |
Note |
TS12: allantois |
Present |
Regionally restricted |
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Co-expression with Mixl1 in ventral allantoic mesothelium.
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Specimen
6F:
embryonic day 8.0; Runx1tm2Spe/Runx1 +
(more )
Note: 6 somite stage. Double labeled: blue - Runx1 reporter; brown - Mixl1 (assay MGI:6436901).
Specimen
6F:
(close )
Genetic Background:
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involves: 129S4/SvJae * C57BL/6J * CBA
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Mutant Allele(s):
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Runx1tm2Spe/Runx1+
|
Age:
|
embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
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Note:
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6 somite stage. Double labeled: blue - Runx1 reporter; brown - Mixl1 (assay MGI:6436901).
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Specimen
6G:
embryonic day 8.0; Runx1tm2Spe/Runx1 +
(more )
Note: 6 somite stage. Double labeled: blue - Runx1 reporter; brown - Mixl1 (assay MGI:6436901).
Specimen
6G:
(close )
Genetic Background:
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involves: 129S4/SvJae * C57BL/6J * CBA
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Mutant Allele(s):
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Runx1tm2Spe/Runx1+
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Age:
|
embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
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Paraffin
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Note:
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6 somite stage. Double labeled: blue - Runx1 reporter; brown - Mixl1 (assay MGI:6436901).
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Specimen
6H:
embryonic day 8.5; Runx1tm2Spe/Runx1 +
(more )
Note: 10 somite stage. Double labeled: blue - Runx1 reporter; brown - Mixl1 (assay MGI:6436901).
Specimen
6H:
(close )
Genetic Background:
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involves: 129S4/SvJae * C57BL/6J * CBA
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Mutant Allele(s):
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Runx1tm2Spe/Runx1+
|
Age:
|
embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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10 somite stage. Double labeled: blue - Runx1 reporter; brown - Mixl1 (assay MGI:6436901).
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: extraembryonic endoderm |
Present |
Regionally restricted |
6H
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Expression colocalizing with Mixl1 expression in extraembryonic visceral endoderm.
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TS13: blood island |
Present |
Regionally restricted |
6H
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Expression colocalizes with Mixl1 expression in presumptive erythroblasts.
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TS13: vitelline artery |
Present |
Regionally restricted |
6H
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Expression localized to developing blood cells of the omphalomesenteric artery.
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TS13: chorion ectoderm |
Weak |
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6H
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Expression to a lesser extent within lateral chorionic ectoderm.
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Specimen
6I:
embryonic day 8.5; Runx1tm2Spe/Runx1 +
(more )
Note: 10 somite stage. Double labeled: blue - Runx1 reporter; brown - Mixl1 (assay MGI:6436901).
Specimen
6I:
(close )
Genetic Background:
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involves: 129S4/SvJae * C57BL/6J * CBA
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Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
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Age:
|
embryonic day 8.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
10 somite stage. Double labeled: blue - Runx1 reporter; brown - Mixl1 (assay MGI:6436901).
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: extraembryonic endoderm |
Present |
Regionally restricted |
6I
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Expression colocalizing with Mixl1 expression in extraembryonic visceral endoderm.
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TS13: yolk sac |
Present |
Regionally restricted |
6I
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Within visceral yolk sac, co-localization with Mixl1 in both endothelium and blood cells.
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TS13: blood island |
Present |
Regionally restricted |
6I
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Expression colocalizes with Mixl1 expression in presumptive erythroblasts. Stronger expression in blood cells.
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Specimen
6J:
embryonic day 8.5; Runx1tm2Spe/Runx1 +
(more )
Note: 9 somite stage. Double labeled: blue - Runx1 reporter; brown - Mixl1 (assay MGI:6436901).
Specimen
6J:
(close )
Genetic Background:
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involves: 129S4/SvJae * C57BL/6J * CBA
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Mutant Allele(s):
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Runx1tm2Spe/Runx1+
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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9 somite stage. Double labeled: blue - Runx1 reporter; brown - Mixl1 (assay MGI:6436901).
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Specimen
6K:
embryonic day 8.5; Runx1tm2Spe/Runx1 +
(more )
Note: 10 somite stage. Double labeled: blue - Runx1 reporter; brown - Mixl1 (assay MGI:6436901).
Specimen
6K:
(close )
Genetic Background:
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involves: 129S4/SvJae * C57BL/6J * CBA
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Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
Age:
|
embryonic day 8.5
|
Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
10 somite stage. Double labeled: blue - Runx1 reporter; brown - Mixl1 (assay MGI:6436901).
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: embryo |
Ambiguous |
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6K
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Authors state sections were treated with brief X-gal exposure. No expression patterns were reported.
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Specimen
6L:
embryonic day 8.5; Runx1tm2Spe/Runx1 +
(more )
Note: 9 somite stage. Double labeled: blue - Runx1 reporter; brown - Mixl1 (assay MGI:6436901).
Specimen
6L:
(close )
Genetic Background:
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involves: 129S4/SvJae * C57BL/6J * CBA
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Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
Age:
|
embryonic day 8.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Paraffin
|
Note:
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9 somite stage. Double labeled: blue - Runx1 reporter; brown - Mixl1 (assay MGI:6436901).
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: embryo |
Ambiguous |
|
6L
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Authors state sections were treated with brief X-gal exposure. No expression patterns were reported.
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