Lef1 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:287334 Wunnemann F, et al., Loss of ADAMTS19 causes progressive non-syndromic heart valve disease. Nat Genet. 2020 Jan;52(1):40-47
Assay type:	RNA in situ
MGI Accession ID:	MGI:6437244
Gene symbol:	Lef1
Gene name:	lymphoid enhancer binding factor 1

Probe:	Mm-Lef1 (441861)
Probe preparation:	Antisense
Visualized with:	Fluorescence
Assay notes:	RNAscope assay; an opal dye was used. The authors provided no catalog id for the probe, but this is the only one sold by Advanced Cell Diagnostics for this mouse gene.

Results

Specimen S12A: embryonic day 12.5 (more

)
Note: Double labeled: red - Lef1; green - Adamts19 (assay MGI:6437241)

Structure	Level	Pattern	Image	Note
TS20: outflow tract endocardial cushion	Present		S12C , S12G' , S12C' , S12G

Specimen S12B: embryonic day 14.5 (more

)
Note: Double labeled: red - Lef1; green - Adamts19 (assay MGI:6437241)

Structure	Level	Pattern	Image	Note
TS22: aortic valve	Present	Regionally restricted	S12H , S12D' , S12D , S12H'	Expression is seen in the interstitial/mesenchymal cells of the valve.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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