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Gene Expression Data
Assay Details
Assay
Reference: J:127471 Wang S, et al., Loss of Myt1 function partially compromises endocrine islet cell differentiation and pancreatic physiological function in the mouse. Mech Dev. 2007 Nov-Dec;124(11-12):898-910
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6449808
Gene symbol: Ins1
Gene name: insulin I
Antibody: anti-insulin
Assay notes: Two antibodies were described, but the one used in this assay was not detailed. Anti-C-peptide MGI:6449771 may have been used in this assay. Both antibodies recognize a related gene; Ins2 (MGI:96573) has also been annotated.
Results
Specimen 1A1 inset: embryonic day 8.5 (more )
Note: Triple labeled: green - Ins1 and Ins2; red - Myt1 (assay MGI:6449800); blue - Pdx1 (assay MGI:6449807).
Structure Level Pattern Image Note
TS13: foregut-midgut junction Absent 1A1 inset No expresssion in prospective pancreatic epithelium.
Specimen 1A1: embryonic day 9.0 (more )
Note: Triple labeled: green - Ins1 and Ins2; red - Myt1 (assay MGI:6449800); blue - Pdx1 (assay MGI:6449807).
Structure Level Pattern Image Note
TS14: foregut-midgut junction Absent 1A1 No expression in prospective pancreas.
Specimen 1A2: embryonic day 10.5 (more )
Note: Triple labeled: green - Ins1 and Ins2; red - Myt1 (assay MGI:6449800); blue - Pdx1 (assay MGI:6449807).
Structure Level Pattern Image Note
TS17: pancreas primordium Present 1A2
Specimen 1A3: embryonic day 15.5 (more )
Note: Double labeled: green - Ins1 and Ins2; red - Myt1 (assay MGI:6449800).
Structure Level Pattern Image Note
TS23: islets of Langerhans Present 1A3
Specimen 1A4: postnatal week 12 (more )
Note: Double labeled: green - Ins1 and Ins2; red - Myt1 (assay MGI:6449800).
Structure Level Pattern Image Note
TS28: islets of Langerhans Present 1A4
Specimen 5A1: embryonic day 15.5 (more )
Note: Double labeled: red - Ins1 and Ins2; green - Ppy (assay MGI:6449810).
Structure Level Pattern Image Note
TS23: islets of Langerhans Present Regionally restricted 5A1 Substantial number of cells co-express insulin and Ppy.
Specimen 5A2: embryonic day 15.5; Myt1tm1.1Ggu/Myt1+ (more )
Note: Double labeled: red - Ins1 and Ins2; green - Ppy (assay MGI:6449810).
Structure Level Pattern Image Note
TS23: islets of Langerhans Present Regionally restricted 5A2 Substantial number of cells co-express insulin and Ppy.
Specimen 5A3: embryonic day 15.5; Myt1tm1.1Ggu/Myt1tm1.1Ggu (more )
Note: Double labeled: red - Ins1 and Ins2; green - Ppy (assay MGI:6449810).
Structure Level Pattern Image Note
TS23: islets of Langerhans Present Regionally restricted 5A3 Substantial number of cells co-express insulin and Ppy.
Specimen 5A3 inset: embryonic day 15.5; Myt1tm1.1Ggu/Myt1tm1.1Ggu (more )
Note: Double labeled: red - Ins1 and Ins2; green - Ppy (assay MGI:6449810).
Structure Level Pattern Image Note
TS23: islets of Langerhans Present 5A3 inset (right)
Specimen 5C1: embryonic day 18.5 (more )
Note: Double labeled: red - Ins1 and Ins2; green - Ppy (assay MGI:6449810).
Structure Level Pattern Image Note
TS26: islets of Langerhans Present Regionally restricted 5C1 Substantial number of cells co-express insulin and Ppy.
Specimen 5C2: embryonic day 18.5; Myt1tm1.1Ggu/Myt1+ (more )
Note: Double labeled: red - Ins1 and Ins2; green - Ppy (assay MGI:6449810).
Structure Level Pattern Image Note
TS26: islets of Langerhans Present Regionally restricted 5C2 Substantial number of cells co-express insulin and Ppy.
Specimen 5C3: embryonic day 18.5; Myt1tm1.1Ggu/Myt1tm1.1Ggu (more )
Note: Double labeled: red - Ins1 and Ins2; green - Ppy (assay MGI:6449810).
Structure Level Pattern Image Note
TS26: islets of Langerhans Present Regionally restricted 5C3 Substantial number of cells co-express insulin and Ppy.
Specimen 5C3 inset: embryonic day 18.5; Myt1tm1.1Ggu/Myt1tm1.1Ggu (more )
Note: Double labeled: red - Ins1 and Ins2; green - Ppy (assay MGI:6449810).
Structure Level Pattern Image Note
TS26: islets of Langerhans Present 5C3 inset (right)
Specimen 6A: postnatal adult; Myt1tm1Ggu/Myt1tm1.1Ggu, Tg(Pdx1-cre)89.1Dam/? (more )
Note: Conditional mutant. Triple labeled: red - Ins1 and Ins2; green - Gcg (assay MGI:6449809); blue - Mafa (assay MGI:6449813).
Structure Level Pattern Image Note
TS28: islets of Langerhans Present Regionally restricted 6A ins , 6A merge Expression in cells that are not mature beta cells.
Specimen 6B: postnatal adult; Myt1tm1Ggu/Myt1tm1.1Ggu, Tg(Pdx1-cre)89.1Dam/? (more )
Note: Conditional mutant. Triple labeled: red - Ins1 and Ins2; green - Ppy (assay MGI:6449810); blue - Mafa (assay MGI:6449813).
Structure Level Pattern Image Note
TS28: islets of Langerhans Present Regionally restricted 6B ins , 6B merge Expression in cells that are not mature beta cells.
Specimen 6C: postnatal adult; Myt1tm1Ggu/Myt1tm1.1Ggu, Tg(Pdx1-cre)89.1Dam/? (more )
Note: Conditional mutant. Triple labeled: red - Ins1 and Ins2; green - Gcg (assay MGI:6449809); blue - Mafb (assay MGI:6449814).
Structure Level Pattern Image Note
TS28: islets of Langerhans Present Regionally restricted 6C merge , 6C ins Expression in cells that are not mature beta cells.
Specimen 6D: postnatal adult; Myt1tm1Ggu/Myt1tm1.1Ggu, Tg(Pdx1-cre)89.1Dam/? (more )
Note: Conditional mutant. Triple labeled: red - Ins1 and Ins2; green - Ppy (assay MGI:6449810); blue - Mafb (assay MGI:6449814).
Structure Level Pattern Image Note
TS28: islets of Langerhans Present Regionally restricted 6D ins , 6D merge Expression in cells that are not mature beta cells.
Specimen 7C1: postnatal month 3 (more )
Note: Double labeled: green - Ins1 and Ins2; red - Glut2 (Slc2a2 assay MGI:6449816).
Structure Level Pattern Image Note
TS28: islets of Langerhans Present 7C1
Specimen 7C2: postnatal month 3; Myt1tm1.1Ggu/Myt1tm1.1Ggu (more )
Note: Double labeled: green - Ins1 and Ins2; red - Glut2 (Slc2a2 assay MGI:6449816).
Structure Level Pattern Image Note
TS28: islets of Langerhans Present 7C2
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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