Krt14 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:228856 DiTommaso T, et al., Keratin 76 is required for tight junction function and maintenance of the skin barrier. PLoS Genet. 2014 Oct;10(10):e1004706
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6456584
Gene symbol:	Krt14
Gene name:	keratin 14

Antibody:

anti-K14 (LL002)

Results

1K KRT76/KRT14 ©

Specimen 1A KRT14: postnatal adult (more

)
Note: Triple labeled: red - Krt14; green - Krt76 (assay MGI:6456583); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: metapodial pad epidermis	Present		1K KRT76/KRT14

1L +/+ ©

Specimen 1L +/+: postnatal adult (more

)
Note: Triple labeled: red - Krt14; green - Krt76 (assay MGI:6456583); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: metapodial pad epidermis	Present		1L +/+

1L tm1a/tm1a ©

Specimen 1L tm1a/tm1a: postnatal adult; Krt76^{tm1a(KOMP)Wtsi}/Krt76^{tm1a(KOMP)Wtsi} (more

)
Note: Triple labeled: red - Krt14; green - Krt76 (assay MGI:6456583); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: metapodial pad epidermis	Present		1L tm1a/tm1a

1N E14.5 ©

Specimen 1N E14.5: embryonic day 14.5 (more

)
Note: Triple labeled: red - Krt14; green - Krt76 (assay MGI:6456583); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS22: back skin	Present		1N E14.5

1N E18.5 ©

Specimen 1N E18.5: embryonic day 18.5 (more

)
Note: Triple labeled: red - Krt14; green - Krt76 (assay MGI:6456583); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS26: back skin	Present		1N E18.5

1N P1 ©

Specimen 1N P1: postnatal day 1 (more

)
Note: Triple labeled: red - Krt14; green - Krt76 (assay MGI:6456583); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS27: back skin	Present		1N P1

1N Adult ©

Specimen 1N Adult: postnatal adult (more

)
Note: Triple labeled: red - Krt14; green - Krt76 (assay MGI:6456583); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: back skin	Present		1N Adult

1N' ©

Specimen 1N': postnatal adult; Krt76^{tm1a(KOMP)Wtsi}/Krt76^{tm1a(KOMP)Wtsi} (more

)
Note: Triple labeled: red - Krt14; green - Krt76 (assay MGI:6456583); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: back skin	Present		1N'

2C +/+ ©

Specimen 2C +/+: postnatal adult (more

)
Note: Triple labeled: red - Krt14; green - phospho-histone H3; blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: back skin	Present		2C +/+

2C tm1a/tm1a ©

Specimen 2C tm1a/tm1a: postnatal adult; Krt76^{tm1a(KOMP)Wtsi}/Krt76^{tm1a(KOMP)Wtsi} (more

)
Note: Triple labeled: red - Krt14; green - phospho-histone H3; blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: back skin	Present		2C tm1a/tm1a

2F (No wound) ©

Specimen 2F (No wound): postnatal adult (more

)
Note: Triple labeled: red - Krt14; green - Krt76 (assay MGI:6456583); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: back skin	Present		2F (No wound)

3A +/+ ©

Specimen 3A +/+: postnatal adult (more

)
Note: Triple labeled: red - Krt14; green - Krt6 (assay MGI:6456586); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: epidermis basal layer	Present		3A +/+

3A (Early) ©

Specimen 3A (Early): postnatal adult; Krt76^{tm1a(KOMP)Wtsi}/Krt76^{tm1a(KOMP)Wtsi} (more

)
Note: Triple labeled: red - Krt14; green - Krt6 (assay MGI:6456586); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: epidermis basal layer	Strong	Regionally restricted	3A (Early)	Authors report normal expression in 'early' phenotype mice.

3A (Late) ©

Specimen 3A (Late): postnatal adult; Krt76^{tm1a(KOMP)Wtsi}/Krt76^{tm1a(KOMP)Wtsi} (more

)
Note: Triple labeled: red - Krt14; green - Krt6 (assay MGI:6456586); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: epidermis	Strong	Regionally restricted	3A (Late)	Expression expanded in interfollicular epidermis of "late" phenotype mice.

3B +/+ ©

Specimen 3B +/+: postnatal adult (more

)
Note: Triple labeled: green - Krt14; red - Fabp5 (assay MGI:6456588); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: back skin	Present		3B +/+

3B (Early) ©

Specimen 3B (Early): postnatal adult; Krt76^{tm1a(KOMP)Wtsi}/Krt76^{tm1a(KOMP)Wtsi} (more

)
Note: Triple labeled: green - Krt14; red - Fabp5 (assay MGI:6456588); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: back skin	Present		3B (Early)

3B (Late) ©

Specimen 3B (Late): postnatal adult; Krt76^{tm1a(KOMP)Wtsi}/Krt76^{tm1a(KOMP)Wtsi} (more

)
Note: Triple labeled: green - Krt14; red - Fabp5 (assay MGI:6456588); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: back skin	Present		3B (Late)

3C +/+ ©

Specimen 3C +/+: postnatal adult (more

)
Note: Triple labeled: red - Krt14; green - Krt10 (assay MGI:6456589); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: back skin	Present		3C +/+

3C (Early) ©

Specimen 3C (Early): postnatal adult; Krt76^{tm1a(KOMP)Wtsi}/Krt76^{tm1a(KOMP)Wtsi} (more

)
Note: Triple labeled: red - Krt14; green - Krt10 (assay MGI:6456589); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: back skin	Present		3C (Early)

3C (Late) ©

Specimen 3C (Late): postnatal adult; Krt76^{tm1a(KOMP)Wtsi}/Krt76^{tm1a(KOMP)Wtsi} (more

)
Note: Triple labeled: red - Krt14; green - Krt10 (assay MGI:6456589); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: back skin	Present		3C (Late)

3D +/+ ©

Specimen 3D +/+: postnatal adult (more

)
Note: Triple labeled: red - Krt14; green - Flg (assay MGI:6456585); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: back skin	Present		3D +/+

3D (Early) ©

Specimen 3D (Early): postnatal adult; Krt76^{tm1a(KOMP)Wtsi}/Krt76^{tm1a(KOMP)Wtsi} (more

)
Note: Triple labeled: red - Krt14; green - Flg (assay MGI:6456585); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: back skin	Present		3D (Early)

3D (Late) ©

Specimen 3D (Late): postnatal adult; Krt76^{tm1a(KOMP)Wtsi}/Krt76^{tm1a(KOMP)Wtsi} (more

)
Note: Triple labeled: red - Krt14; green - Flg (assay MGI:6456585); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: back skin	Present		3D (Late)

3G +/+ ©

Specimen 3G +/+: postnatal adult (more

)
Note: Triple labeled: green - Krt14; red - Fasn (assay MGI:6456590); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: back skin	Present		3G +/+

3G (Early) ©

Specimen 3G (Early): postnatal adult; Krt76^{tm1a(KOMP)Wtsi}/Krt76^{tm1a(KOMP)Wtsi} (more

)
Note: Triple labeled: green - Krt14; red - Fasn (assay MGI:6456590); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: back skin	Present		3G (Early)

3G (Late) ©

Specimen 3G (Late): postnatal adult; Krt76^{tm1a(KOMP)Wtsi}/Krt76^{tm1a(KOMP)Wtsi} (more

)
Note: Triple labeled: green - Krt14; red - Fasn (assay MGI:6456590); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: back skin	Present		3G (Late)

3H +/+ ©

Specimen 3H +/+: postnatal adult (more

)
Note: Triple labeled: green - Krt14; red - MEL-A (Mlana; assay MGI:6456591); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: back skin	Present		3H +/+

3H (Early) ©

Specimen 3H (Early): postnatal adult; Krt76^{tm1a(KOMP)Wtsi}/Krt76^{tm1a(KOMP)Wtsi} (more

)
Note: Triple labeled: green - Krt14; red - MEL-A (Mlana; assay MGI:6456591); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: back skin	Present		3H (Early)

3H (Late) ©

Specimen 3H (Late): postnatal adult; Krt76^{tm1a(KOMP)Wtsi}/Krt76^{tm1a(KOMP)Wtsi} (more

)
Note: Triple labeled: green - Krt14; red - MEL-A (Mlana; assay MGI:6456591); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: back skin	Present		3H (Late)

S2B +/+ ©

Specimen S2B +/+: postnatal adult (more

)
Note: Triple labeled: red - Krt14; green - ZO-1 (Tjp1; assay MGI:6456598); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: metapodial pad	Present		S2B +/+

Specimen S2B tm1a/tm1a: postnatal adult; Krt76^{tm1a(KOMP)Wtsi}/Krt76^{tm1a(KOMP)Wtsi} (more

)
Note: Triple labeled: red - Krt14; green - ZO-1 (Tjp1; assay MGI:6456598); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: metapodial pad epidermis	Present		S2B tm1a/tm1a

S2F No wound ©

Specimen S2F tm1a/tm1a: postnatal adult; Krt76^{tm1a(KOMP)Wtsi}/Krt76^{tm1a(KOMP)Wtsi} (more

)
Note: Triple labeled: green - Krt14; red - ZO-1 (Tjp1; assay MGI:6456598); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: back skin	Present		S2F No wound

S3A +/+ ©

Specimen S3A +/+: postnatal adult (more

)
Note: Triple labeled: red - Krt14; green - Ocln (assay MGI:6456599); blue - DAPI

Structure	Level	Pattern	Image	Note
TS28: metapodial pad	Present		S3A +/+

Specimen S3A tm1a/tm1a: postnatal adult; Krt76^{tm1a(KOMP)Wtsi}/Krt76^{tm1a(KOMP)Wtsi} (more

)
Note: Triple labeled: red - Krt14; green - Ocln (assay MGI:6456599); blue - DAPI

Structure	Level	Pattern	Image	Note
TS28: metapodial pad	Present		S3A tm1a/tm1a

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