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Gene Expression Data
Assay Details
Assay
Reference: J:245763 Gao L, et al., Afadin orients cell division to position the tubule lumen in developing renal tubules. Development. 2017 Oct 01;144(19):3511-3520
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6467813
Gene symbol: Ncam1
Gene name: neural cell adhesion molecule 1
Antibody: 5B8 antibody
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 1A f/f 4um: embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Note: Double labeled: red - Ncam1; blue - Pard6b (assay MGI:6467789).
Structure Level Pattern Image Note
TS25: s-shaped body Present 1A f/f-1 Expressed in the basolateral junction.
Specimen 1A f/f 11um: embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Note: Double labeled: red - Ncam1; blue - Pard6b (assay MGI:6467789).
Structure Level Pattern Image Note
TS25: s-shaped body Present 1A f/f-2 Expressed in the basolateral junction.
Specimen 1A f/f 20um: embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Note: Double labeled: red - Ncam1; blue - Pard6b (assay MGI:6467789).
Structure Level Pattern Image Note
TS25: s-shaped body Present 1A f/f-3 Expressed in the basolateral junction.
Specimen 1A f/f 3D: embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Note: Double labeled: red - Ncam1; blue - Pard6b (assay MGI:6467789).
Structure Level Pattern Image Note
TS25: s-shaped body Present 1A f/f-4 Expressed in the basolateral junction.
Specimen 1A cKO 4um: embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: red - Ncam1; blue - Pard6b (assay MGI:6467789).
Structure Level Pattern Image Note
TS25: s-shaped body Present 1A cKO-1 Expressed in the basolateral junction.
Specimen 1A cKO 11um: embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: red - Ncam1; blue - Pard6b (assay MGI:6467789).
Structure Level Pattern Image Note
TS25: s-shaped body Present 1A cKO-2 Expressed in the basolateral junction.
Specimen 1A cKO 20um: embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: red - Ncam1; blue - Pard6b (assay MGI:6467789).
Structure Level Pattern Image Note
TS25: s-shaped body Present 1A cKO-3 Expressed in the basolateral junction.
Specimen 1A cKO 3D: embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: red - Ncam1; blue - Pard6b (assay MGI:6467789).
Structure Level Pattern Image Note
TS25: s-shaped body Present 1A cKO-4 Expressed in the basolateral junction.
Specimen 1C: embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: red - Ncam1; blue - Pard6b (assay MGI:6467789).
Structure Level Pattern Image Note
TS25: s-shaped body Present 1C Expressed in the basolateral junction.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/10/2024
MGI 6.24 		The Jackson Laboratory