About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:245763 Gao L, et al., Afadin orients cell division to position the tubule lumen in developing renal tubules. Development. 2017 Oct 01;144(19):3511-3520
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6467814
Gene symbol: Ncam1
Gene name: neural cell adhesion molecule 1
Antibody: 5B8 antibody
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 5A/A': embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Note: Triple labeled: green - Ncam1; red - alpha-tubulin; blue - Pard6b (assay MGI:6467789).
Structure Level Pattern Image Note
TS25: s-shaped body Present 5A , 5A' Expressed in the basolateral junction. The orientation of division appeared to be parallel to the luminal/apical surface.
Specimen 5B/B': embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Note: Triple labeled: green - Ncam1; red - alpha-tubulin; blue - Pard6b (assay MGI:6467789).
Structure Level Pattern Image Note
TS25: s-shaped body Present 5B , 5B' Expressed in the basolateral junction. The orientation of division appeared to be parallel to the luminal/apical surface.
Specimen 5C/C': embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Note: Triple labeled: green - Ncam1; red - alpha-tubulin; blue - Pard6b (assay MGI:6467789).
Structure Level Pattern Image Note
TS25: s-shaped body Present 5C , 5C' Expressed in the basolateral junction. The midbody was formed apically.
Specimen S2A f/f left: postnatal day 0; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Note: Double labeled: green - Ncam1; red - Afdn (assay MGI:6467823).
Structure Level Pattern Image Note
TS27: s-shaped body Present S2A upper left Expressed in the basolateral junction.
Specimen S2A f/f right: postnatal day 0; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Note: Double labeled: green - Ncam1; red - Afdn (assay MGI:6467823).
Structure Level Pattern Image Note
TS27: s-shaped body Present S2A upper right Expressed in the basolateral junction.
Specimen S2A cKO left: postnatal day 0; Afdntm1.1Lfr/Afdntm1.1Lfr, Wnt4tm3(EGFP/cre)Amc/Wnt4+ (more )
Note: Conditional mutant. Double labeled: green - Ncam1; red - Afdn (assay MGI:6467823).
Structure Level Pattern Image Note
TS27: s-shaped body Present S2A lower left Expressed in the basolateral junction.
Specimen S2A cKO right: postnatal day 0; Afdntm1.1Lfr/Afdntm1.1Lfr, Wnt4tm3(EGFP/cre)Amc/Wnt4+ (more )
Note: Conditional mutant. Double labeled: green - Ncam1; red - Afdn (assay MGI:6467823).
Structure Level Pattern Image Note
TS27: s-shaped body Present S2A lower right Expressed in the basolateral junction.
Specimen S2B f/f left: postnatal day 0; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Note: Double labeled: green - Ncam1; red - Afdn (assay MGI:6467823).
Structure Level Pattern Image Note
TS27: s-shaped body Present S2B upper left Expressed in the basolateral junction.
Specimen S2B cKO left: postnatal day 0; Afdntm1.1Lfr/Afdntm1.1Lfr, Wnt4tm3(EGFP/cre)Amc/Wnt4+ (more )
Note: Conditional mutant. Double labeled: green - Ncam1; red - Afdn (assay MGI:6467823).
Structure Level Pattern Image Note
TS27: s-shaped body Present S2B lower left Expressed in the basolateral junction.
Specimen S3Ba: postnatal newborn; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Note: Double labeled: green - Ncam1; red - Afdn (assay MGI:6467823).
Structure Level Pattern Image Note
TS27: s-shaped body Present S3Ba right Expressed in the basolateral junction.
Specimen S3Bb: postnatal newborn; Afdntm1.1Lfr/Afdntm1.1Lfr, Tg(Hoxb7-cre)13Amc/0 (more )
Note: Conditional mutant. Double labeled: green - Ncam1; red - Afdn (assay MGI:6467823).
Structure Level Pattern Image Note
TS27: s-shaped body Present S3Bb right Expressed in the basolateral junction.
Specimen S3Bc: postnatal newborn; Afdntm1.1Lfr/Afdntm1.1Lfr, Tg(Hoxb7-cre)13Amc/0 (more )
Note: Conditional mutant. Double labeled: green - Ncam1; red - Afdn (assay MGI:6467823).
Structure Level Pattern Image Note
TS27: s-shaped body Present S3Bc right Expressed in the basolateral junction.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
12/10/2024
MGI 6.24 		The Jackson Laboratory