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Gene Expression Data
Assay Details
Assay
Reference: J:294176 Snyder E, et al., ADAD1 and ADAD2, testis-specific adenosine deaminase domain-containing proteins, are required for male fertility. Sci Rep. 2020 Jul 14;10(1):11536
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6472899
Gene symbol: Ddx25
Gene name: DEAD box helicase 25
Results
Specimen 5A XI WT: postnatal adult (more )
Note: Late pachytene spermatocytes (Stage XI). Double labeled: red - Ddx25; green - Smg1 (assay MGI:6472902).
Structure Level Pattern Image Note
TS28: spermatocyte Present 5A WT XI DDX25 , 5A WT XI Merge Expression is in cytoplasmic granules in late pachytene spermatocytes.
Specimen 5A IV WT: postnatal adult (more )
Note: Early round spermatids (Stage IV). Double labeled: red - Ddx25; green - Smg1 (assay MGI:6472902).
Structure Level Pattern Image Note
TS28: spermatid Present 5A WT IV Merge , 5A WT IV DDX25 Expression is in cytoplasmic granules in early round spermatids.
Specimen 5A XI Adad2em3/em3: postnatal adult; Adad2em3(IMPC)J/Adad2em3(IMPC)J (more )
Note: Late pachytene spermatocytes (Stage XI). Double labeled: red - Ddx25; green - Smg1 (assay MGI:6472902).
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 5A Adad2em3/em3 XI Merge , 5A Adad2em3/em3 XI DDX25 Expression in this mutant is the same as in wild-type.
Specimen 5A IV Adad2em3/em3: postnatal adult; Adad2em3(IMPC)J/Adad2em3(IMPC)J (more )
Note: Early round spermatids (Stage IV). Double labeled: red - Ddx25; green - Smg1 (assay MGI:6472902).
Structure Level Pattern Image Note
TS28: spermatid Present Regionally restricted 5A Adad2em3/em3 IV Merge , 5A Adad2em3/em3 IV DDX25 Expression in this mutant is the same as in wild-type.
Specimen 5B: postnatal adult; Adad2em3(IMPC)J/Adad2em3(IMPC)J (more )
Note: Double labeled: red - Ddx25; green - Adad2 (assay MGI:6472838).
Structure Level Pattern Image Note
TS28: spermatocyte Present 5B DDX25 , 5B Merge
Specimen 5C Stage VII: postnatal adult (more )
Note: Double labeled: red - Ddx25; green - Adad2 (assay MGI:6472838).
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 5C VII Merge , 5C VII DDX25 Expression is in granules in stage IX pachytene spermatocytes.
Specimen 5C Stage IX: postnatal adult (more )
Note: Double labeled: red - Ddx25; green - Adad2 (assay MGI:6472838).
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 5C IX Merge , 5C IX DDX25 Expression is in granules in stage IX pachytene spermatocytes.
Specimen 5C Stage XI: postnatal adult (more )
Note: Double labeled: red - Ddx25; green - Adad2 (assay MGI:6472838).
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 5C XI Merge , 5C XI DDX25 Expression is in granules in stage IX pachytene spermatocytes.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/17/2024
MGI 6.24 		The Jackson Laboratory