About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:178848 An CI, et al., Genome-wide mapping of Sox6 binding sites in skeletal muscle reveals both direct and indirect regulation of muscle terminal differentiation by Sox6. BMC Dev Biol. 2011;11:59
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6491594
Gene symbol: Myh7
Gene name: myosin, heavy polypeptide 7, cardiac muscle, beta
Antibody: Anti-beta-MHC NOQ7.5.4D (M8421, MAB1628)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1A Sox6f/f: embryonic day 18.5; Sox6tm2.1Vlf/Sox6tm2.1Vlf (more )
Note: Double labeled: green - Myh7; red - Sox6 (assay MGI:6491845).
Structure Level Pattern Image Note
TS26: extensor digitorum longus Present 1A Soxf/f Merge , 1A Soxf/f MyHC-beta
TS26: tibialis anterior Present 1A Soxf/f Merge , 1A Soxf/f MyHC-beta
Specimen 1A Sox6f/f;Myf5-cre: embryonic day 18.5; Myf5tm3(cre)Sor/Myf5+, Sox6tm2.1Vlf/Sox6tm2.1Vlf (more )
Note: Conditional mutant. Double labeled: green - Myh7; red - Sox6 (assay MGI:6491845).
Structure Level Pattern Image Note
TS26: extensor digitorum longus Strong 1A Sox6f/f;Myf5-Cre Merge , 1A Sox6f/f;Myf5-Cre MyHC-beta Nearly 100% of myofibers had expression.
TS26: tibialis anterior Strong 1A Sox6f/f;Myf5-Cre Merge , 1A Sox6f/f;Myf5-Cre MyHC-beta Nearly 100% of myofibers had expression.
Specimen 1B Sox6f/f: postnatal day 7; Sox6tm2.1Vlf/Sox6tm2.1Vlf (more )
Note: Double labeled: green - Myh7; red - Sox6 (assay MGI:6491845).
Structure Level Pattern Image Note
TS28: extensor digitorum longus Present 1B Soxf/f Merge , 1B Soxf/f MyHC-beta
TS28: tibialis anterior Present 1B Soxf/f Merge , 1B Soxf/f MyHC-beta
Specimen 1B Sox6f/f;Myf5-cre: postnatal day 7; Myf5tm3(cre)Sor/Myf5+, Sox6tm2.1Vlf/Sox6tm2.1Vlf (more )
Note: Conditional mutant. Double labeled: green - Myh7; red - Sox6 (assay MGI:6491845).
Structure Level Pattern Image Note
TS28: extensor digitorum longus Strong 1B Sox6f/f;Myf5-Cre MyHC-beta , 1B Sox6f/f;Myf5-Cre Merge Nearly 100% of myofibers had expression.
TS28: tibialis anterior Strong 1B Sox6f/f;Myf5-Cre MyHC-beta , 1B Sox6f/f;Myf5-Cre Merge Nearly 100% of myofibers had expression.
Specimen 1C Sox6f/f: postnatal month 4; Sox6tm2.1Vlf/Sox6tm2.1Vlf (more )
Note: Double labeled: green - Myh7; red - Sox6 (assay MGI:6491845).
Structure Level Pattern Image Note
TS28: extensor digitorum longus Weak 1C Soxf/f MyHC-beta , 1C Soxf/f Merge
TS28: tibialis anterior Weak 1C Soxf/f MyHC-beta , 1C Soxf/f Merge
Specimen 1C Sox6f/f;Myf5-cre: postnatal month 4; Myf5tm3(cre)Sor/Myf5+, Sox6tm2.1Vlf/Sox6tm2.1Vlf (more )
Note: Conditional mutant. Double labeled: green - Myh7; red - Sox6 (assay MGI:6491845).
Structure Level Pattern Image Note
TS28: extensor digitorum longus Present Regionally restricted 1C Sox6f/f;Myf5-Cre Merge , 1C Sox6f/f;Myf5-Cre MyHC-beta Approximately 50% of myofibers were positive compared to controls.
TS28: tibialis anterior Present Regionally restricted 1C Sox6f/f;Myf5-Cre Merge , 1C Sox6f/f;Myf5-Cre MyHC-beta Approximately 50% of myofibers were positive compared to controls.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
06/12/2024
MGI 6.13 		The Jackson Laboratory