Pou5f1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:231231 Abby E, et al., Implementation of meiosis prophase I programme requires a conserved retinoid-independent stabilizer of meiotic transcripts. Nat Commun. 2016;7:10324
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6705056
Gene symbol:	Pou5f1
Gene name:	POU domain, class 5, transcription factor 1

Antibody:	anti-Oct3/4 (C-10; sc-5279)
Detection system:	Secondary antibody coupled to horseradish peroxidase

Results

Specimen 4a +/+ VASA/OCT4: embryonic day 14.5 (more

)
Note: Double labeled: brown - Pou5f1; magenta - Ddx4 (assay MGI:6695761).

Structure	Level	Pattern	Image	Note
TS22: premeiotic germ cell of ovary	Present		4a Meioc+/+ Vasa/Oct4

Specimen 4a -/- VASA/OCT4: embryonic day 14.5; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Double labeled: brown - Pou5f1; magenta - Ddx4 (assay MGI:6695761).

Structure	Level	Pattern	Image	Note
TS22: premeiotic germ cell of ovary	Present		4a Meioc-/- Vasa/Oct4

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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