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Gene Expression Data
Assay Details
Assay
Reference: J:243650 Herriges MJ, et al., The NANCI-Nkx2.1 gene duplex buffers Nkx2.1 expression to maintain lung development and homeostasis. Genes Dev. 2017 May 01;31(9):889-903
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6863930
Gene symbol: Foxj1
Gene name: forkhead box J1
Antibody: anti-Foxj1
Detection system: Secondary antibody
Results
Specimen 1F - Foxj1: postnatal adult; Sfta3tm1.1(cre/ERT2)Eem/Sfta3+ (more )
Note: Double labeled: green - Foxj1; red - Sfta3-ps reporter (assay MGI:6863897).
Structure Level Pattern Image Note
TS28: lung epithelium Present Single cells 1F bottom merge , 1F bottom Lineage Marker Expression is seen in ciliated cells.
Specimen 1H - Foxj1: postnatal adult (more )
Note: Double labeled: green - Foxj1; red - Nkx2-1 (assay MGI:6863898).
Structure Level Pattern Image Note
TS28: lung epithelium Present Single cells 1H bottom merge , 1H bottom Lineage Marker Expression is seen in ciliated cells.
Specimen 3B - Wild-type: embryonic day 18.5 (more )
Note: Double labeled: red - Foxj1; green - Scgb1a1 (assay MGI:6863929).
Structure Level Pattern Image Note
TS26: lung epithelium Present 3B upper Wild-type Expression is seen in ciliated cells.
Specimen 3B - NANCIcreERT2-RFP/+: embryonic day 18.5; Sfta3tm1.1(cre/ERT2)Eem/Sfta3+ (more )
Note: Double labeled: red - Foxj1; green - Scgb1a1 (assay MGI:6863929).
Structure Level Pattern Image Note
TS26: lung epithelium Present 3B upper NANCIRFP/+ Expression is seen in ciliated cells; their numbers are comparable to those seen in wild type.
Specimen 3B - Nkx2.1GFP/+: embryonic day 18.5; Nkx2-1tm1Ikono/Nkx2-1+ (more )
Note: Double labeled: red - Foxj1; green - Scgb1a1 (assay MGI:6863929).
Structure Level Pattern Image Note
TS26: lung epithelium Present 3B upper Nkx2.1GFP/+ Expression is seen in ciliated cells; their numbers are significantly reduced compared to those seen in wild type.
Specimen 3B - NANCIcreERT2-RFP/+; Nkx2.1GFP/+: embryonic day 18.5; Nkx2-1tm1Ikono/Nkx2-1+, Sfta3tm1.1(cre/ERT2)Eem/Sfta3+ (more )
Note: Double labeled: red - Foxj1; green - Scgb1a1 (assay MGI:6863929).
Structure Level Pattern Image Note
TS26: lung epithelium Present 3B upper NANCIRFP/+; Nkx2.1GFP/+ Expression is seen in ciliated cells; their numbers are significantly reduced compared to those seen in wild type.
Specimen 3E - Wild-type: postnatal day 4 (more )
Note: Double labeled: red - Foxj1; green - Scgb1a1 (assay MGI:6863929).
Structure Level Pattern Image Note
TS28: lung epithelium Present 3E upper Wild-type Expression is seen in ciliated cells.
Specimen 3E - NANCIcreERT2-RFP/+: postnatal day 4; Sfta3tm1.1(cre/ERT2)Eem/Sfta3+ (more )
Note: Double labeled: red - Foxj1; green - Scgb1a1 (assay MGI:6863929).
Structure Level Pattern Image Note
TS28: lung epithelium Present 3E upper NANCIRFP/+ Expression is seen in ciliated cells; their numbers are comparable to those seen in wild type.
Specimen 3E - Nkx2.1GFP/+: postnatal day 4; Nkx2-1tm1Ikono/Nkx2-1+ (more )
Note: Double labeled: red - Foxj1; green - Scgb1a1 (assay MGI:6863929).
Structure Level Pattern Image Note
TS28: lung epithelium Present 3E upper Nkx2.1GFP/+ Expression is seen in ciliated cells; unlike earlier in development, their numbers are comparable to those seen in wild type.
Specimen 3E - NANCIcreERT2-RFP/+; Nkx2.1GFP/+: postnatal day 4; Nkx2-1tm1Ikono/Nkx2-1+, Sfta3tm1.1(cre/ERT2)Eem/Sfta3+ (more )
Note: Double labeled: red - Foxj1; green - Scgb1a1 (assay MGI:6863929).
Structure Level Pattern Image Note
TS28: lung epithelium Present 3E upper NANCIRFP/+; Nkx2.1GFP/+ Expression is seen in ciliated cells; their numbers are significantly reduced compared to those seen in wild type.
Specimen 5D: postnatal week 10; Nkx2-1tm1Ikono/Nkx2-1+, Sfta3tm1.1(cre/ERT2)Eem/Sfta3+ (more )
Note: Double labeled: green - Foxj1; red - Sox2 (assay MGI:6863946).
Structure Level Pattern Image Note
TS28: lung epithelium Present 5D Expression is not seen in the bronchiolized cells in the distal lung.
Specimen 6C - Wild-type: postnatal month 6 (more )
Note: Double labeled: red - Foxj1; green - Scgb1a1 (assay MGI:6863929).
Structure Level Pattern Image Note
TS28: lung epithelium Present 6C top Wild-type
Specimen 6C - NANCIcreERT2-RFP/+: postnatal month 6; Sfta3tm1.1(cre/ERT2)Eem/Sfta3+ (more )
Note: Double labeled: red - Foxj1; green - Scgb1a1 (assay MGI:6863929).
Structure Level Pattern Image Note
TS28: lung epithelium Present 6C top NANCIRFP/+ Expression levels are not reduced in the mutant.
Specimen 6C - Nkx2.1GFP/+: postnatal month 6; Nkx2-1tm1Ikono/Nkx2-1+ (more )
Note: Double labeled: red - Foxj1; green - Scgb1a1 (assay MGI:6863929).
Structure Level Pattern Image Note
TS28: lung epithelium Present 6C top Nkx2.1GFP/+ Expression levels are not reduced in the mutant.
Specimen 6C - NANCIcreERT2-RFP/+; Nkx2.1GFP/+: postnatal month 6; Nkx2-1tm1Ikono/Nkx2-1+, Sfta3tm1.1(cre/ERT2)Eem/Sfta3+ (more )
Note: Double labeled: red - Foxj1; green - Scgb1a1 (assay MGI:6863929).
Structure Level Pattern Image Note
TS28: lung epithelium Present 6C top NANCIRFP/+; Nkx2.1GFP/+ Expression levels are not reduced in the mutant.
Specimen 6D: postnatal month 6; Nkx2-1tm1Ikono/Nkx2-1+, Sfta3tm1.1(cre/ERT2)Eem/Sfta3+ (more )
Note: Double labeled: red - Foxj1; green - Scgb1a1 (assay MGI:6863929).
Structure Level Pattern Image Note
TS28: lung epithelium Present Single cells 6D Expression is seen in ciliated cells in the distal bronchiolized epithelium.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/10/2024
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