Trp63 Immunohistochemistry Gene Expression Assay - GXD

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Gene Expression Data
Assay Details

Assay

Reference:	J:243650 Herriges MJ, et al., The NANCI-Nkx2.1 gene duplex buffers Nkx2.1 expression to maintain lung development and homeostasis. Genes Dev. 2017 May 01;31(9):889-903
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6863935
Gene symbol:	Trp63
Gene name:	transformation related protein 63

Antibody:	anti-P63
Detection system:	Secondary antibody

Results

S3E bottom Lineage Marker ©

S3E bottom merge ©

Specimen S3E - P63: postnatal adult; Sfta3^{tm1.1(cre/ERT2)Eem}/Sfta3⁺ (more

)
Note: Double labeled: green - Trp63; red - Sfta3-ps reporter (assay MGI:6863897).

Structure	Level	Pattern	Image	Note
TS28: lobar bronchus epithelium	Present	Single cells	S3E bottom Lineage Marker , S3E bottom merge	Expression is seen in basal cells of the mainstem bronchi.

S3F bottom Lineage Marker ©

S3F bottom merge ©

Specimen S3F - P63: postnatal adult (more

)
Note: Double labeled: green - Trp63; red - Nkx2-1 (assay MGI:6863898).

Structure	Level	Pattern	Image	Note
TS28: lobar bronchus epithelium	Present	Single cells	S3F bottom merge , S3F bottom Lineage Marker	Expression is seen in basal cells of the mainstem bronchi.

5A upper Wild-type ©

Specimen 5A - Wild-type: postnatal week 10 (more

)
Note: Double labeled: green - Trp63; red - Krt5 (assay MGI:6863942).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Absent		5A upper Wild-type

5A upper NANCI^RFP/+ ©

Specimen 5A - NANCI^{creERT2-RFP/+}: postnatal week 10; Sfta3^{tm1.1(cre/ERT2)Eem}/Sfta3⁺ (more

)
Note: Double labeled: green - Trp63; red - Krt5 (assay MGI:6863942).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Absent		5A upper NANCI^RFP/+

5A upper Nkx2.1^GFP/+ ©

Specimen 5A - Nkx2.1^GFP/+: postnatal week 10; Nkx2-1^tm1Ikono/Nkx2-1⁺ (more

)
Note: Double labeled: green - Trp63; red - Krt5 (assay MGI:6863942).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Absent		5A upper Nkx2.1^GFP/+

5A upper NANCI^RFP/+; Nkx2.1^GFP/+ ©

Specimen 5A - NANCI^{creERT2-RFP/+}; Nkx2.1^GFP/+: postnatal week 10; Nkx2-1^tm1Ikono/Nkx2-1⁺, Sfta3^{tm1.1(cre/ERT2)Eem}/Sfta3⁺ (more

)
Note: Double labeled: green - Trp63; red - Krt5 (assay MGI:6863942).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present		5A upper NANCI^RFP/+; Nkx2.1^GFP/+	This expression is an indication of basal cell metaplasia.

6C center Wild-type ©

Specimen 6C - Wild-type: postnatal month 6 (more

)
Note: Double labeled: green - Trp63; red - Krt5 (assay MGI:6863942).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Absent		6C center Wild-type

6C center NANCI^RFP/+ ©

Specimen 6C - NANCI^{creERT2-RFP/+}: postnatal month 6; Sfta3^{tm1.1(cre/ERT2)Eem}/Sfta3⁺ (more

)
Note: Double labeled: green - Trp63; red - Krt5 (assay MGI:6863942).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Absent		6C center NANCI^RFP/+

6C center Nkx2.1^GFP/+ ©

Specimen 6C - Nkx2.1^GFP/+: postnatal month 6; Nkx2-1^tm1Ikono/Nkx2-1⁺ (more

)
Note: Double labeled: green - Trp63; red - Krt5 (assay MGI:6863942).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Absent		6C center Nkx2.1^GFP/+

6C center NANCI^RFP/+; Nkx2.1^GFP/+ ©

Specimen 6C - NANCI^{creERT2-RFP/+}; Nkx2.1^GFP/+: postnatal month 6; Nkx2-1^tm1Ikono/Nkx2-1⁺, Sfta3^{tm1.1(cre/ERT2)Eem}/Sfta3⁺ (more

)
Note: Double labeled: green - Trp63; red - Krt5 (assay MGI:6863942).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present	Single cells	6C center NANCI^RFP/+; Nkx2.1^GFP/+	Basal cell expression is expanded in the mutant.

6E ©

Specimen 6E: postnatal month 6; Nkx2-1^tm1Ikono/Nkx2-1⁺, Sfta3^{tm1.1(cre/ERT2)Eem}/Sfta3⁺ (more

)
Note: Double labeled: green - Trp63; red - Krt5 (assay MGI:6863942).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present	Single cells	6E	Expression is seen in basal-like cells in the distal bronchiolized epithelium.

6F ©

Specimen 6F: postnatal month 6; Nkx2-1^tm1Ikono/Nkx2-1⁺, Sfta3^{tm1.1(cre/ERT2)Eem}/Sfta3⁺ (more

)
Note: Double labeled: green - Trp63; red - Nkx2-1 (assay MGI:6863898).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present	Single cells	6F	Expression is seen in basal-like cells in the distal bronchiolized epithelium.

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