Pecam1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:316418 Azzoni E, et al., The onset of circulation triggers a metabolic switch required for endothelial to hematopoietic transition. Cell Rep. 2021 Dec 14;37(11):110103
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7276240
Gene symbol:	Pecam1
Gene name:	platelet/endothelial cell adhesion molecule 1

Antibody:	anti-CD31 (AF3628)
Detection system:	Secondary antibody

Results

1A CD31/Runx1 +/+ (left) ©

Specimen 1A +/+ CD31: embryonic day 8.25 (more

)
Note: 4-6 somite pairs. Double labeled: white - CD31 (Pecam1); green - Runx1 (assay MGI:7276241).

Structure	Level	Pattern	Image	Note
TS12: dorsal aorta	Present		1A CD31/Runx1 +/+ (left)
TS12: vitelline artery	Present		1A CD31/Runx1 +/+ (left)

1A CD31/Runx1 -/- (left) ©

Specimen 1A -/- CD31: embryonic day 8.25; Slc8a1^tm1Sjc/Slc8a1^tm1Sjc (more

)
Note: 4-6 somite pairs. Double labeled: white - CD31 (Pecam1); green - Runx1 (assay MGI:7276241).

Structure	Level	Pattern	Image	Note
TS12: dorsal aorta	Present		1A CD31/Runx1 -/- (left)
TS12: vitelline artery	Present		1A CD31/Runx1 -/- (left)

2A CD31/CD41/Runx1 +/+ ©

Specimen 2A +/+ CD31: embryonic day 9.5 (more

)
Note: 21-26 somite pairs. Triple labeled: white - CD31 (Pecam1); red - CD41 (Itga2b assay MGI:7276245); green - Runx1 (assay MGI:7276241).

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta endothelium	Present	Regionally restricted	2A CD31/CD41/Runx1 +/+	Expression in hemogenic endothelium.

2A CD31/CD41/Runx1 -/- ©

Specimen 2A -/- CD31: embryonic day 9.5; Slc8a1^tm1Sjc/Slc8a1^tm1Sjc (more

)
Note: 21-26 somite pairs. Triple labeled: white - CD31 (Pecam1); red - CD41 (Itga2b assay MGI:7276245); green - Runx1 (assay MGI:7276241).

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta endothelium	Present	Regionally restricted	2A CD31/CD41/Runx1 -/-	Expression in hemogenic endothelium.

3A +/+ (left) ©

Specimen 3A +/+ CD31: embryonic day 9.5 (more

)
Note: 22-25 somite pairs. Triple labeled: white - CD31 (Pecam1); red - alpha-SMA (Acta2 assay MGI:7276247); green - Runx1 (assay MGI:7276241).

Structure	Level	Pattern	Image	Note
TS15: embryo	Present		3A +/+ (left)

3A -/- (left) ©

Specimen 3A -/- CD31: embryonic day 9.5; Slc8a1^tm1Sjc/Slc8a1^tm1Sjc (more

)
Note: 22-25 somite pairs. Triple labeled: white - CD31 (Pecam1); red - alpha-SMA (Acta2 assay MGI:7276247); green - Runx1 (assay MGI:7276241).

Structure	Level	Pattern	Image	Note
TS15: embryo	Present		3A -/- (left)

3A +/+ (middle) ©

Specimen 3A +/+ slice CD31: embryonic day 9.5 (more

)
Note: 22-25 somite pairs. Triple labeled: white - CD31 (Pecam1); red - alpha-SMA (Acta2 assay MGI:7276247); green - Runx1 (assay MGI:7276241).

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta	Present		3A +/+ (middle)

3A -/- (middle) ©

Specimen 3A -/- slice CD31: embryonic day 9.5; Slc8a1^tm1Sjc/Slc8a1^tm1Sjc (more

)
Note: 22-25 somite pairs. Triple labeled: white - CD31 (Pecam1); red - alpha-SMA (Acta2 assay MGI:7276247); green - Runx1 (assay MGI:7276241).

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta	Present		3A -/- (middle)

3E +/+ ©

Specimen 3E +/+ CD31: embryonic day 9.5 (more

)
Note: 21-24 somite pairs. Triple labeled: white - CD31 (Pecam1); red - F4/80 (Adgre1 assay MGI:7276248); green - Runx1 (assay MGI:7276241).

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta	Present		3E +/+

3E -/- ©

Specimen 3E -/- CD31: embryonic day 9.5; Slc8a1^tm1Sjc/Slc8a1^tm1Sjc (more

)
Note: 21-24 somite pairs. Triple labeled: white - CD31 (Pecam1); red - F4/80 (Adgre1 assay MGI:7276248); green - Runx1 (assay MGI:7276241).

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta	Present		3E -/-

S1B +/+ ©

Specimen S1B +/+: embryonic day 9.5 (more

)
Note: 23-24 somite pairs.

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta	Present		S1B +/+
TS15: vitelline artery	Present		S1B +/+

S1B -/- ©

Specimen S1B -/-: embryonic day 9.5; Slc8a1^tm1Sjc/Slc8a1^tm1Sjc (more

)
Note: 23-24 somite pairs.

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta	Present		S1B -/-
TS15: vitelline artery	Present		S1B -/-

Specimen S1D +/+ CD31: embryonic day 9.5 (more

)
Note: 21-26 somite pairs. Triple labeled: white - CD31 (Pecam1); red - c-Kit (Kit assay MGI:7276248); green - Runx1 (assay MGI:7276241).

Structure	Level	Pattern	Image	Note
TS15: yolk sac	Present		S1D CD31/c-Kit/Runx1 +/+

Specimen S1D -/- CD31: embryonic day 9.5; Slc8a1^tm1Sjc/Slc8a1^tm1Sjc (more

)
Note: 21-26 somite pairs. Triple labeled: white - CD31 (Pecam1); red - c-Kit (Kit assay MGI:7276248); green - Runx1 (assay MGI:7276241).

Structure	Level	Pattern	Image	Note
TS15: yolk sac	Present		S1D CD31/c-Kit/Runx1 -/-

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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