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Gene Expression Data
Assay Details
Assay
Reference: J:316418 Azzoni E, et al., The onset of circulation triggers a metabolic switch required for endothelial to hematopoietic transition. Cell Rep. 2021 Dec 14;37(11):110103
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7276243
Gene symbol: Dll4
Gene name: delta like canonical Notch ligand 4
Antibody: Human/Mouse DLL4 Antibody (MAB1389)
Detection system: Secondary antibody
Results
Specimen 1A +/+ DLL4: embryonic day 8.25 (more )
Note: 4-6 somite pairs. Double labeled: red - Dll4; white - VE-Cad (Cdh5 assay MGI:7276242).
Structure Level Pattern Image Note
TS12: dorsal aorta Present 1A VE-Cad/DLL4 +/+ (left) , 1A VE-Cad/DLL4 +/+ (right)
TS12: vitelline artery Present 1A VE-Cad/DLL4 +/+ (left) , 1A VE-Cad/DLL4 +/+ (right)
Specimen 1A -/- DLL4: embryonic day 8.25; Slc8a1tm1Sjc/Slc8a1tm1Sjc (more )
Note: 4-6 somite pairs. Double labeled: red - Dll4; white - VE-Cad (Cdh5 assay MGI:7276242).
Structure Level Pattern Image Note
TS12: dorsal aorta Present 1A VE-Cad/DLL4 -/- (right) , 1A VE-Cad/DLL4 -/- (left)
TS12: vitelline artery Present 1A VE-Cad/DLL4 -/- (right) , 1A VE-Cad/DLL4 -/- (left)
Specimen 1C +/+ DLL4: embryonic day 9.5 (more )
Note: 22-26 somite pairs. Double labeled: red - Dll4; white - VE-Cad (Cdh5 assay MGI:7276242).
Structure Level Pattern Image Note
TS15: dorsal aorta Present 1C +/+ (left) , 1C +/+ (middle left)
TS15: umbilical artery Present 1C +/+ (left) , 1C +/+ (middle left)
TS15: vitelline artery Present 1C +/+ (left) , 1C +/+ (middle left)
Specimen 1C -/- DLL4: embryonic day 9.5; Slc8a1tm1Sjc/Slc8a1tm1Sjc (more )
Note: 22-26 somite pairs. Double labeled: red - Dll4; white - VE-Cad (Cdh5 assay MGI:7276242).
Structure Level Pattern Image Note
TS15: dorsal aorta Present 1C -/- (left) , 1C -/- (middle left)
TS15: umbilical artery Present 1C -/- (left) , 1C -/- (middle left)
TS15: vitelline artery Present 1C -/- (left) , 1C -/- (middle left)
Specimen 1C +/+ slice DLL4: embryonic day 9.5 (more )
Note: 22-26 somite pairs. Double labeled: red - Dll4; white - VE-Cad (Cdh5 assay MGI:7276242).
Structure Level Pattern Image Note
TS15: dorsal aorta endothelium Present 1C +/+ (middle right)
Specimen 1C -/- slice DLL4: embryonic day 9.5 (more )
Note: 22-26 somite pairs. Double labeled: red - Dll4; white - VE-Cad (Cdh5 assay MGI:7276242).
Structure Level Pattern Image Note
TS15: dorsal aorta endothelium Present 1C -/- (middle right) Expression relatively unperturbed.
Specimen 1C +/+ inset DLL4: embryonic day 9.5 (more )
Note: 22-26 somite pairs. Double labeled: red - Dll4; white - VE-Cad (Cdh5 assay MGI:7276242).
Structure Cell Type Level Pattern Image Note
TS15: dorsal aorta endothelium aortic endothelial cell Present 1C +/+ (right)
Specimen 1C -/- inset DLL4: embryonic day 9.5 (more )
Note: 22-26 somite pairs. Double labeled: red - Dll4; white - VE-Cad (Cdh5 assay MGI:7276242).
Structure Cell Type Level Pattern Image Note
TS15: dorsal aorta endothelium aortic endothelial cell Present 1C -/- (right)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
12/10/2024
MGI 6.24 		The Jackson Laboratory