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Gene Expression Data
Assay Details
Assay
Reference: J:316418 Azzoni E, et al., The onset of circulation triggers a metabolic switch required for endothelial to hematopoietic transition. Cell Rep. 2021 Dec 14;37(11):110103
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7276244
Gene symbol: Spn
Gene name: sialophorin
Antibody: anti-CD43 (M19)
Detection system: Secondary antibody
Results
Specimen 2A +/+ (left) CD43: embryonic day 9.5 (more )
Note: 21-26 somite pairs. Triple labeled: red - CD43 (Spn); white - VE-Cad (Cdh5 assay MGI:7276242); green - Runx1 (assay MGI:7276241).
Structure Level Pattern Image Note
TS15: dorsal aorta endothelium Present Regionally restricted 2A VE-Cad/CD43/Runx1 +/+ (left) Expression in hemogenic endothelium.
Specimen 2A -/- (left) CD43: embryonic day 9.5; Slc8a1tm1Sjc/Slc8a1tm1Sjc (more )
Note: 21-26 somite pairs. Triple labeled: red - CD43 (Spn); white - VE-Cad (Cdh5 assay MGI:7276242); green - Runx1 (assay MGI:7276241).
Structure Level Pattern Image Note
TS15: dorsal aorta Absent 2A VE-Cad/CD43/runx1 -/- (left)
Specimen 2A +/+ (right) CD43: embryonic day 9.5 (more )
Note: 21-26 somite pairs. Triple labeled: red - CD43 (Spn); white - VE-Cad (Cdh5 assay MGI:7276242); green - Runx1 (assay MGI:7276241).
Structure Cell Type Level Pattern Image Note
TS15: vitelline artery hematopoietic cell Present 2A VE-Cad/CD43/Runx1 +/+ (right)
Specimen 2A -/- (right) CD43: embryonic day 9.5; Slc8a1tm1Sjc/Slc8a1tm1Sjc (more )
Note: 21-26 somite pairs. Triple labeled: red - CD43 (Spn); white - VE-Cad (Cdh5 assay MGI:7276242); green - Runx1 (assay MGI:7276241).
Structure Cell Type Level Pattern Image Note
TS15: vitelline artery hematopoietic cell Absent 2A VE-Cad/CD43/Runx1 -/- (right) Authors report absence of hematopoietic cell clusters.
Specimen S1D +/+ CD43: embryonic day 9.5 (more )
Note: 21-26 somite pairs. Triple labeled: red - CD43 (Spn); white - VE-Cad (Cdh5 assay MGI:7276242); green - Runx1 (assay MGI:7276241).
Structure Level Pattern Image Note
TS15: yolk sac Present S1D VE-Cad/CD43/Runx1 +/+
Specimen S1D -/- CD43: embryonic day 9.5 (more )
Note: 21-26 somite pairs. Triple labeled: red - CD43 (Spn); white - VE-Cad (Cdh5 assay MGI:7276242); green - Runx1 (assay MGI:7276241).
Structure Level Pattern Image Note
TS15: yolk sac Present S1D VE-Cad/CD43/Runx1 -/-
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
12/17/2024
MGI 6.24 		The Jackson Laboratory