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Gene Expression Data
Assay Details
Assay
Reference: J:316418 Azzoni E, et al., The onset of circulation triggers a metabolic switch required for endothelial to hematopoietic transition. Cell Rep. 2021 Dec 14;37(11):110103
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7276247
Gene symbol: Acta2
Gene name: actin alpha 2, smooth muscle, aorta
Antibody: Anti-smooth muscle alpha-Actin (clone 1A4)
Detection system: Secondary antibody
Results
Specimen 3A +/+ alpha-SMA: embryonic day 9.5 (more )
Note: 22-25 somite pairs. Triple labeled: red - alpha-SMA (Acta2); white - CD31 (Pecam1 assay MGI:7276240); green - Runx1 (assay MGI:7276241).
Structure Level Pattern Image Note
TS15: embryo Present 3A +/+ (left)
Specimen 3A -/- alpha-SMA: embryonic day 9.5; Slc8a1tm1Sjc/Slc8a1tm1Sjc (more )
Note: 22-25 somite pairs. Triple labeled: red - alpha-SMA (Acta2); white - CD31 (Pecam1 assay MGI:7276240); green - Runx1 (assay MGI:7276241).
Structure Level Pattern Image Note
TS15: embryo Present Regionally restricted 3A -/- (left) Expression lost in cells around dorsal aorta.
Specimen 3A +/+ slice alpha-SMA: embryonic day 9.5 (more )
Note: 22-25 somite pairs. Triple labeled: red - alpha-SMA (Acta2); white - CD31 (Pecam1 assay MGI:7276240); green - Runx1 (assay MGI:7276241).
Structure Cell Type Level Pattern Image Note
TS15: heart Present 3A +/+ (middle) , 3A +/+ (right)
TS15: vitelline artery Present 3A +/+ (middle) , 3A +/+ (right)
TS15: dorsal aorta aortic smooth muscle cell Present 3A +/+ (middle) , 3A +/+ (right)
Specimen 3A -/- slice alpha-SMA: embryonic day 9.5; Slc8a1tm1Sjc/Slc8a1tm1Sjc (more )
Note: 22-25 somite pairs. Triple labeled: red - alpha-SMA (Acta2); white - CD31 (Pecam1 assay MGI:7276240); green - Runx1 (assay MGI:7276241).
Structure Cell Type Level Pattern Image Note
TS15: heart Present 3A -/- (right) , 3A -/- (middle)
TS15: vitelline artery Present 3A -/- (right) , 3A -/- (middle)
TS15: dorsal aorta aortic smooth muscle cell Absent 3A -/- (right) , 3A -/- (middle)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/10/2024
MGI 6.24 		The Jackson Laboratory