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Gene Expression Data
Assay Details
Assay
Reference: J:307824 Liu H, et al., The Scleraxis Transcription Factor Directly Regulates Multiple Distinct Molecular and Cellular Processes During Early Tendon Cell Differentiation. Front Cell Dev Biol. 2021;9:654397
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7284079
Gene symbol: Scx
Gene name: scleraxis scleraxis bHLH transcription factor
Reporter: FLAG
Antibody: anti-FLAG M2 antibody (F1804)
Detection system: Secondary antibody coupled to Alexa Fluor 568
Results
Specimen 1G: embryonic day 16.5; Scxem1Jian/Scx+ (more )
Note: Double labeled: red - Scx; green - Sox9 (assay MGI:7284182).
Structure Level Pattern Image Note
TS24: forelimb tendon Strong Regionally restricted 1G Expressed in patellar tendons.
TS24: cruciate ligament of knee Strong 1G
Specimen 1H: embryonic day 16.5; Scxem1Jian/Scx+ (more )
Note: Double labeled: red - Scx; green - Sox9 (assay MGI:7284182).
Structure Level Pattern Image Note
TS24: forelimb tendon Strong Regionally restricted 1H Expressed in Achilles tendons and smaller tendons next to the Achilles.
Specimen 1I: embryonic day 16.5; Scxem1Jian/Scx+ (more )
Note: Double labeled: red - Scx; green - Sox9 (assay MGI:7284182).
Structure Level Pattern Image Note
TS24: forelimb tendon Strong Regionally restricted 1I Expressed in all the autopod tendons.
Specimen 1J: embryonic day 16.5; Scxem1Jian/Scx+ (more )
Note: Double labeled: red - Scx; green - Sox9 (assay MGI:7284182).
Structure Level Pattern Image Note
TS24: forelimb tendon Strong Regionally restricted 1J Expressed in all the zeugopod tendons.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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06/12/2024
MGI 6.13 		The Jackson Laboratory