Ncam1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:240523 Yang Z, et al., Role of CD34 family members in lumen formation in the developing kidney. Dev Biol. 2016 Oct 01;418(1):66-74
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7346139
Gene symbol:	Ncam1
Gene name:	neural cell adhesion molecule 1

Antibody:	anti-NCAM [H28.123]
Detection system:	Secondary antibody

Results

1B left ©

1B middle ©

Specimen 1B: postnatal day 0 (more

)
Note: Cap mesenchyme stage. Triple labeled: green - Ncam1; red - Podxl2 (assay MGI:7346135); blue - Prkci (assay MGI:7346141).

Structure	Level	Pattern	Image	Note
TS27: cap mesenchyme	Present		1B right upper , 1B middle , 1B left	Expressed in the plasma membrane.

1C left ©

Specimen 1C: postnatal day 0 (more

)
Note: Pretubular aggregate stage. Double labeled: green - Ncam1; red - Podxl2 (assay MGI:7346135).

Structure	Level	Pattern	Image	Note
TS27: pretubular aggregate	Present		1C right middle , 1C left , 1C right merge	Expressed in the plasma membrane.

1D left ©

Specimen 1D: postnatal day 0 (more

)
Note: Renal vesicle stage. Double labeled: green - Ncam1; red - Podxl2 (assay MGI:7346135). Cyan - nuclear staining.

Structure	Level	Pattern	Image	Note
TS27: renal vesicle	Present		1D left , 1D right middle	Expression became restricted to the lateral surfaces.

5C left ©

Specimen 5C CTL: embryonic day 14.5; Podxl^tm1Parl/Podxl^tm1Parl, Podxl2^{tm1c(EUCOMM)Wtsi}/Podxl2^{tm1c(EUCOMM)Wtsi} (more

)
Note: Double labeled: green - Ncam1; red - Cd34 (assay MGI:7346202).

Structure	Level	Pattern	Image	Note
TS22: s-shaped body	Present		5C left	Expressed in the plasma membrane.

5C right ©

Specimen 5C cKO: embryonic day 14.5; Podxl^tm1Parl/Podxl^tm1Parl, Podxl2^{tm1c(EUCOMM)Wtsi}/Podxl2^{tm1c(EUCOMM)Wtsi}, Tg(Pax3-cre)1Joe/0 (more

)
Note: Conditional mutant. Double labeled: green - Ncam1; red - Cd34 (assay MGI:7346202).

Structure	Level	Pattern	Image	Note
TS22: s-shaped body	Present		5C right	Expressed in the plasma membrane, similar as in the control.

5D left ©

Specimen 5D CTL: embryonic day 14.5; Podxl^tm1Parl/Podxl^tm1Parl, Podxl2^{tm1c(EUCOMM)Wtsi}/Podxl2^{tm1c(EUCOMM)Wtsi} (more

)
Note: Double labeled: green - Ncam1; red - Pard6b (assay MGI:7346205).

Structure	Level	Pattern	Image	Note
TS22: s-shaped body	Present		5D left	Expressed in the plasma membrane.

5D right ©

Specimen 5D cKO: embryonic day 14.5; Podxl^tm1Parl/Podxl^tm1Parl, Podxl2^{tm1c(EUCOMM)Wtsi}/Podxl2^{tm1c(EUCOMM)Wtsi}, Tg(Pax3-cre)1Joe/0 (more

)
Note: Conditional mutant. Double labeled: green - Ncam1; red - Pard6b (assay MGI:7346205).

Structure	Level	Pattern	Image	Note
TS22: s-shaped body	Present		5D right	Expressed in the plasma membrane, similar as in the control.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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