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Gene Expression Data
Assay Details
Assay
Reference: J:240523 Yang Z, et al., Role of CD34 family members in lumen formation in the developing kidney. Dev Biol. 2016 Oct 01;418(1):66-74
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7346144
Gene symbol: Podxl
Gene name: podocalyxin-like
Antibody: anti-Podxl (MAB1556)
Detection system: Secondary antibody
Results
Specimen 1G: postnatal day 0 (more )
Note: Pretubular aggregate stage. Triple labeled: red - Podxl; green - Podxl2 (assay MGI:7346137); blue - Prkci (assay MGI:7346141).
Structure Level Pattern Image Note
TS27: pretubular aggregate Absent 1G right lower , 1G left
Specimen 1H: postnatal day 0 (more )
Note: Renal vesicle stage. Triple labeled: red - Podxl; green - Podxl2 (assay MGI:7346137); blue - Prkci (assay MGI:7346141).
Structure Level Pattern Image Note
TS27: renal vesicle Weak Regionally restricted 1H right lower , 1H left Expressed at the luminal surface.
Specimen 1I: postnatal day 0 (more )
Note: S-shaped body stage. Triple labeled: red - Podxl; green - Podxl2 (assay MGI:7346137); blue - Prkci (assay MGI:7346141).
Structure Cell Type Level Pattern Image Note
TS27: s-shaped body podocyte Weak Regionally restricted 1I left , 1I right Expressed at the apical surface, especially at the apical surface of podocyte precursors.
TS27: renal capillary endothelial cell Present 1I left , 1I right Expression was more abundant compared with the s-shaped body lumen.
Specimen 1J: postnatal day 0 (more )
Note: Early glomeruli stage. Double labeled: red - Podxl; green - anti-F-actin antibody.
Structure Cell Type Level Pattern Image Note
TS27: renal capillary endothelial cell Present 1J left , 1J right
TS27: capillary loop renal corpuscle podocyte Present Regionally restricted 1J left , 1J right Increased expression at the apical surface of podocytes, exceeding levels in the endothelia.
Specimen 5B CTL: embryonic day 14.5; Podxltm1Parl/Podxltm1Parl, Podxl2tm1c(EUCOMM)Wtsi/Podxl2tm1c(EUCOMM)Wtsi (more )
Note: Double labeled: red - Podxl; green - anti-F-actin antibody.
Structure Cell Type Level Pattern Image Note
TS22: s-shaped body podocyte Present 5B left
TS22: renal small blood vessel endothelial cell Present 5B left
Specimen 5B cKO: embryonic day 14.5; Podxltm1Parl/Podxltm1Parl, Podxl2tm1c(EUCOMM)Wtsi/Podxl2tm1c(EUCOMM)Wtsi, Tg(Pax3-cre)1Joe/0 (more )
Note: Conditional mutant. Double labeled: red - Podxl; green - anti-F-actin antibody.
Structure Cell Type Level Pattern Image Note
TS22: s-shaped body podocyte Absent 5B right
TS22: renal small blood vessel endothelial cell Present 5B right
Specimen not shown: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: cap mesenchyme Absent
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/10/2024
MGI 6.24 		The Jackson Laboratory