Gene Expression Data
Assay Details
Assay
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Reference:
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J:329558
Liu W, et al., Expression of Calbindin-D28K in the Developing and Adult Mouse Cochlea. J Histochem Cytochem. 2022 Aug;70(8):583-596
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7356366
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Gene symbol:
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Sox2
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Gene name:
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SRY (sex determining region Y)-box 2
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Results
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Specimen
1B:
embryonic day 16.5
(more )
Note: In apical turn of cochlea. Double labeled: red - Sox2; green - Calb1 (assay MGI:7356365).
Specimen
1B:
(close )
Genetic Background:
|
BALB/c
|
Age:
|
embryonic day 16.5
|
Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
|
Type:
|
optical section
|
Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
Note:
|
In apical turn of cochlea. Double labeled: red - Sox2; green - Calb1 (assay MGI:7356365).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: greater epithelial ridge |
Present |
Regionally restricted |
1B
|
Expression in portion of greater epithelial ridge. No co-expression with Calb1.
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Specimen
1C:
embryonic day 16.5
(more )
Note: In middle turn of cochlea. Double labeled: red - Sox2; green - Calb1 (assay MGI:7356365).
Specimen
1C:
(close )
Genetic Background:
|
BALB/c
|
Age:
|
embryonic day 16.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
In middle turn of cochlea. Double labeled: red - Sox2; green - Calb1 (assay MGI:7356365).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: greater epithelial ridge |
Present |
Regionally restricted |
1C
|
Expression localized in most external part of greater epithelial ridge. No co-expression with Calb1.
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Specimen
1D:
embryonic day 16.5
(more )
Note: In middle turn of cochlea. Double labeled: red - Sox2; green - Calb1 (assay MGI:7356365).
Specimen
1D:
(close )
Genetic Background:
|
BALB/c
|
Age:
|
embryonic day 16.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
In middle turn of cochlea. Double labeled: red - Sox2; green - Calb1 (assay MGI:7356365).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS24: organ of Corti |
cochlear inner hair cell |
Present |
Regionally restricted |
1D
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Expression in hair cell and supporting cell nuclei.
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TS24: organ of Corti |
cochlear outer hair cell |
Present |
Regionally restricted |
1D
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Expression in hair cell and supporting cell nuclei.
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TS24: organ of Corti |
Deiter's cell |
Present |
Regionally restricted |
1D
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Expression in hair cell and supporting cell nuclei.
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Specimen
1E:
embryonic day 16.5
(more )
Note: Double labeled: red - Sox2; green - Calb1 (assay MGI:7356365).
Specimen
1E:
(close )
Genetic Background:
|
BALB/c
|
Age:
|
embryonic day 16.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Sox2; green - Calb1 (assay MGI:7356365).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: cochlea |
Present |
Regionally restricted |
1E
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Expression in glial cells of Rosenthal's canal.
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Specimen
1F:
embryonic day 18.5
(more )
Note: In apical turn of cochlea. Double labeled: red - Sox2; green - Calb1 (assay MGI:7356365).
Specimen
1F:
(close )
Genetic Background:
|
BALB/c
|
Age:
|
embryonic day 18.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
In apical turn of cochlea. Double labeled: red - Sox2; green - Calb1 (assay MGI:7356365).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: organ of Corti |
cochlear inner hair cell |
Absent |
|
1F
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In apical turn of cochlea, inner hair cells remain unlabelled.
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Specimen
1G:
embryonic day 18.5
(more )
Note: In middle turn of cochlea. Double labeled: red - Sox2; green - Calb1 (assay MGI:7356365).
Specimen
1G:
(close )
Genetic Background:
|
BALB/c
|
Age:
|
embryonic day 18.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
In middle turn of cochlea. Double labeled: red - Sox2; green - Calb1 (assay MGI:7356365).
|
Specimen
1I:
embryonic day 18.5
(more )
Note: In basal turn of cochlea. Double labeled: red - Sox2; green - Calb1 (assay MGI:7356365).
Specimen
1I:
(close )
Genetic Background:
|
BALB/c
|
Age:
|
embryonic day 18.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
In basal turn of cochlea. Double labeled: red - Sox2; green - Calb1 (assay MGI:7356365).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: cochlea |
Deiter's cell |
Present |
|
1I
|
|
Specimen
2B:
postnatal day 1
(more )
Note: Double labeled: red - Sox2; green - Calb1 (assay MGI:7356365).
Specimen
2B:
(close )
Genetic Background:
|
BALB/c
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Sox2; green - Calb1 (assay MGI:7356365).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: greater epithelial ridge |
Present |
Regionally restricted |
2B
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Expression domain extends medially into medial portion of greater epithelial ridge.
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Specimen
3B:
postnatal day 5
(more )
Note: Double labeled: red - Sox2; green - Calb1 (assay MGI:7356365).
Specimen
3B:
(close )
Genetic Background:
|
BALB/c
|
Age:
|
postnatal day 5
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Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Sox2; green - Calb1 (assay MGI:7356365).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: greater epithelial ridge |
Present |
Regionally restricted |
3B
|
Expression domain extends medially into medial portion of greater epithelial ridge.
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Specimen
3H:
postnatal day 8
(more )
Note: Double labeled: red - Sox2; green - Calb1 (assay MGI:7356365).
Specimen
3H:
(close )
Genetic Background:
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BALB/c
|
Age:
|
postnatal day 8
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Sex:
|
Not Specified
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Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Sox2; green - Calb1 (assay MGI:7356365).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: greater epithelial ridge |
Present |
Regionally restricted |
3H
|
Expression domain extends medially into medial portion of greater epithelial ridge.
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