About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:331142 Han P, et al., Cbln1 regulates axon growth and guidance in multiple neural regions. PLoS Biol. 2022 Nov;20(11):e3001853
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7388461
Gene symbol: Isl2
Gene name: insulin related protein 2 (islet 2)
Antibody: anti-Isl1/2
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen S1E Isl1/2: embryonic day 11.0 (more )
Note: Triple labeled: blue - Isl1 and Isl2; green - Cbln1 (assay MGI:7388453); red - red - Pou4f1 (assay MGI:7388458).
Structure Cell Type Level Pattern Image Note
TS18: future spinal cord commissural neuron Present Regionally restricted S1E (merged) , S1E Isl1/2 Expression in dI3 dorsal commissural neurons.
Specimen S2E Cbln1fl/fl Isl1/2: embryonic day 11.5; Cbln1tm1.1Sjj/Cbln1tm1.1Sjj (more )
Note: Double labeled: green - Isl1 and Isl2; red - Pou4f1 (assay MGI:7388458).
Structure Cell Type Level Pattern Image Note
TS19: future spinal cord commissural neuron Present Regionally restricted S2E Cbln1fl/fl (merged) , S2E Cbln1fl/fl Isl1/2 Expression in dI3 dorsal commissural neurons.
Specimen S2E Wnt1-Cre Isl1/2: embryonic day 11.5; Cbln1tm1.1Sjj/Cbln1tm1.1Sjj, H2az2Tg(Wnt1-cre)11Rth/0 (more )
Note: Conditional mutant. Double labeled: green - Isl1 and Isl2; red - Pou4f1 (assay MGI:7388458).
Structure Cell Type Level Pattern Image Note
TS19: future spinal cord commissural neuron Present Regionally restricted S2E Wnt1-Cre Isl1/2 , S2E Wnt1-Cre (merged) Patterning not affected for dI3 neurons.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
12/10/2024
MGI 6.24 		The Jackson Laboratory