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Gene Expression Data
Assay Details
Assay
Reference: J:320419 Li R, et al., E3 ubiquitin ligase FBXW7 balances airway cell fates. Dev Biol. 2022 Mar;483:89-97
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7427627
Gene symbol: Scgb1a1
Gene name: secretoglobin, family 1A, member 1
Antibody: CCSP antibody (WRAB-3950)
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 2A: embryonic day 18.5; Fbxw7tm1Iaai/Fbxw7+, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant. Double labeled: green - Scgb1a1; red - Foxj1 (assay MGI:7427628).
Structure Cell Type Level Pattern Image Note
TS26: trachea epithelium club cell Present 2A
Specimen 2B: embryonic day 18.5; Fbxw7tm1Iaai/Fbxw7tm1Iaai, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant. Double labeled: green - Scgb1a1; red - Foxj1 (assay MGI:7427628).
Structure Cell Type Level Pattern Image Note
TS26: trachea epithelium club cell Absent 2B Drastic loss of positive cells compared with the control.
Specimen 4G: embryonic day 18.5; Fbxw7tm1Iaai/Fbxw7+, Notch1tm1Agt/Notch1+, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant. Double labeled: green - Scgb1a1; red - Foxj1 (assay MGI:7427628).
Structure Cell Type Level Pattern Image Note
TS26: trachea epithelium club cell Present 4G
Specimen 4H: embryonic day 18.5; Fbxw7tm1Iaai/Fbxw7tm1Iaai, Notch1tm1Agt/Notch1+, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant. Double labeled: green - Scgb1a1; red - Foxj1 (assay MGI:7427628).
Structure Cell Type Level Pattern Image Note
TS26: trachea epithelium club cell Absent 4H Drastic loss of positive cells compared with the control.
Specimen 4I: embryonic day 18.5; Fbxw7tm1Iaai/Fbxw7tm1Iaai, Notch1tm1Agt/Notch1tm1Agt, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant. Double labeled: green - Scgb1a1; red - Foxj1 (assay MGI:7427628).
Structure Cell Type Level Pattern Image Note
TS26: trachea epithelium club cell Absent 4I Drastic loss of positive cells compared with the control persisted.
Specimen 5G: embryonic day 18.5; Fbxw7tm1Iaai/Fbxw7+, Klf5tm1Jaw/Klf5+, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant. Double labeled: green - Scgb1a1; red - Foxj1 (assay MGI:7427628).
Structure Cell Type Level Pattern Image Note
TS26: trachea epithelium club cell Present 5G
Specimen 5H: embryonic day 18.5; Fbxw7tm1Iaai/Fbxw7tm1Iaai, Klf5tm1Jaw/Klf5+, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant. Double labeled: green - Scgb1a1; red - Foxj1 (assay MGI:7427628).
Structure Cell Type Level Pattern Image Note
TS26: trachea epithelium club cell Absent 5H
Specimen 5I: embryonic day 18.5; Fbxw7tm1Iaai/Fbxw7tm1Iaai, Klf5tm1Jaw/Klf5tm1Jaw, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant. Double labeled: green - Scgb1a1; red - Foxj1 (assay MGI:7427628).
Structure Cell Type Level Pattern Image Note
TS26: trachea epithelium club cell Absent 5I Club cells were still absent in the Fbxw7:Klf5 compound mutant.
Specimen S1E: embryonic day 18.5; Fbxw7tm1Iaai/Fbxw7+, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant. Double labeled: green - Scgb1a1; red - Acta2 (assay MGI:7427660).
Structure Cell Type Level Pattern Image Note
TS26: trachea epithelium club cell Present S1E
Specimen S1F: embryonic day 18.5; Fbxw7tm1Iaai/Fbxw7tm1Iaai, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant. Double labeled: green - Scgb1a1; red - Acta2 (assay MGI:7427660).
Structure Cell Type Level Pattern Image Note
TS26: trachea epithelium club cell Absent S1F Club cells were absent on both the dorsal and the ventral trachea epithelium.
Specimen S3A: embryonic day 18.5; Fbxw7tm1Iaai/Fbxw7+, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant. Double labeled: green - Scgb1a1; red - Foxj1 (assay MGI:7427628).
Structure Cell Type Level Pattern Image Note
TS26: lung epithelium club cell Present S3A
Specimen S3B: embryonic day 18.5; Fbxw7tm1Iaai/Fbxw7tm1Iaai, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant. Double labeled: green - Scgb1a1; red - Foxj1 (assay MGI:7427628).
Structure Cell Type Level Pattern Image Note
TS26: lung epithelium club cell Present S3B Increased positive club cells compared with the control.
Specimen S4D: embryonic day 18.5; Fbxw7tm1Iaai/Fbxw7+, Shhtm1(EGFP/cre)Cjt/Shh+, Tgif1tm1Caw/Tgif1+ (more )
Note: Conditional mutant. Double labeled: green - Scgb1a1; red - Foxj1 (assay MGI:7427628).
Structure Cell Type Level Pattern Image Note
TS26: trachea epithelium club cell Present S4D
Specimen S4E: embryonic day 18.5; Fbxw7tm1Iaai/Fbxw7tm1Iaai, Shhtm1(EGFP/cre)Cjt/Shh+, Tgif1tm1Caw/Tgif1+ (more )
Note: Conditional mutant. Double labeled: green - Scgb1a1; red - Foxj1 (assay MGI:7427628).
Structure Cell Type Level Pattern Image Note
TS26: trachea epithelium club cell Absent S4E Drastic loss of positive cells compared with the control.
Specimen S4F: embryonic day 18.5; Fbxw7tm1Iaai/Fbxw7tm1Iaai, Shhtm1(EGFP/cre)Cjt/Shh+, Tgif1tm1Caw/Tgif1tm1Caw (more )
Note: Conditional mutant. Double labeled: green - Scgb1a1; red - Foxj1 (assay MGI:7427628).
Structure Cell Type Level Pattern Image Note
TS26: trachea epithelium club cell Absent S4F Drastic loss of positive cells persisted, similar to the Fbxw7 single mutant.
Specimen S5E: embryonic day 18.5; Fbxw7tm1Iaai/Fbxw7+, Notch1tm1Agt/Notch1+, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant. Double labeled: green - Scgb1a1; red - Foxj1 (assay MGI:7427628).
Structure Cell Type Level Pattern Image Note
TS26: trachea epithelium club cell Present S5E
Specimen S5F: embryonic day 18.5; Fbxw7tm1Iaai/Fbxw7+, Notch1tm1Agt/Notch1tm1Agt, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant. Double labeled: green - Scgb1a1; red - Foxj1 (assay MGI:7427628).
Structure Cell Type Level Pattern Image Note
TS26: trachea epithelium club cell Present S5F Similar as in the control.
Specimen S6E: embryonic day 18.5; Fbxw7tm1Iaai/Fbxw7+, Klf5tm1Jaw/Klf5+, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant. Double labeled: green - Scgb1a1; red - Foxj1 (assay MGI:7427628).
Structure Cell Type Level Pattern Image Note
TS26: trachea epithelium club cell Present S6E
Specimen S6F: embryonic day 18.5; Fbxw7tm1Iaai/Fbxw7+, Klf5tm1Jaw/Klf5tm1Jaw, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant. Double labeled: green - Scgb1a1; red - Foxj1 (assay MGI:7427628).
Structure Cell Type Level Pattern Image Note
TS26: trachea epithelium club cell Absent S6F
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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09/17/2024
MGI 6.24 		The Jackson Laboratory