Shh RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:200708 Jin JZ, et al., Cripto is required for mesoderm and endoderm cell allocation during mouse gastrulation. Dev Biol. 2013 Sep 1;381(1):170-8
Assay type:	RNA in situ
MGI Accession ID:	MGI:7429037
Gene symbol:	Shh
Gene name:	sonic hedgehog

Probe:	Shh probe224
Probe preparation:	Antisense RNA

Results

4A ©

Specimen 4A: embryonic day 8.5 (more

)
Note: Multi-labeled: dark purple - Shh; dark purple - Otx2 (assay MGI:7429035), Meox1 (assay MGI:7429036); red - Myl7 (assay MGI:7429041).

Structure	Level	Image
TS13: axial mesoderm	Present	4A
TS13: foregut diverticulum endoderm	Present	4A
TS13: notochord	Present	4A

4B ©

Specimen 4B: embryonic day 8.5 (more

)
Note: Triple labeled: dark purple - Shh; dark purple - Meox1 (assay MGI:7429036); red - Myl7 (assay MGI:7429041).

Structure	Level	Image
TS13: axial mesoderm	Present	4B
TS13: foregut diverticulum endoderm	Present	4B
TS13: notochord	Present	4B

4D ©

Specimen 4D: embryonic day 8.5; Edil3^{Tg(Sox2-cre)1Amc}/Edil3⁺, Cripto^tm1Mms/Cripto^tm2.2Mms (more

)
Note: Conditional mutant. Multi-labeled: dark purple - Shh; dark purple - Otx2 (assay MGI:7429035), Meox1 (assay MGI:7429036); red - Myl7 (assay MGI:7429041).

Structure	Level	Image
TS13: axial mesoderm	Present	4D
TS13: foregut diverticulum endoderm	Present	4D
TS13: notochord	Present	4D

4E ©

Specimen 4E: embryonic day 8.5; Edil3^{Tg(Sox2-cre)1Amc}/Edil3⁺, Cripto^tm1Mms/Cripto^tm2.2Mms (more

)
Note: Conditional mutant. Triple labeled: dark purple - Shh; dark purple - Meox1 (assay MGI:7429036); red - Myl7 (assay MGI:7429041).

Structure	Level	Image	Note
TS13: axial mesoderm	Present	4E
TS13: notochord	Present	4E
TS13: foregut diverticulum endoderm	Present	4E	Foregut endoderm defects, in particular defects in the ventral closure of the foregut were seen.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 09/17/2024 MGI 6.24