Calb1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:283394 Hughes LJ, et al., Yap/Taz are required for establishing the cerebellar radial glia scaffold and proper foliation. Dev Biol. 2020 Jan 1;457(1):150-162
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7434575
Gene symbol:	Calb1
Gene name:	calbindin 1

Antibody:	clone CB-955 (#C9848)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

7G WT ©

Specimen 7G WT: postnatal day 10 (more

)
Note: Double labeled: green - Calb1; red - S100b (assay MGI:7434456).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Purkinje cell	Present		7G WT

7G CKO ©

Specimen 7G dCKO: postnatal day 10; Tg(Nes-cre)1Atp/0, Wwtr1^tm1.1Eno/Wwtr1^tm1.1Eno, Yap1^tm1.1Eno/Yap1^tm1.1Eno (more

)
Note: Conditional mutant. Double labeled: green - Calb1; red - S100b (assay MGI:7434456).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Purkinje cell	Present		7G CKO	Smaller lobules II and III compared with the wild-type.

S4C ©

S4C' ©

Specimen S4C/C': postnatal day 0 (more

)
Note: Double labeled: green - Calb1; red - Fabp7 (assay MGI:7434455).

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Purkinje cell	Present		S4C' , S4C

S4D ©

S4D' ©

Specimen S4D/D': postnatal day 0; Tg(Nes-cre)1Atp/0, Wwtr1^tm1.1Eno/Wwtr1^tm1.1Eno, Yap1^tm1.1Eno/Yap1^tm1.1Eno (more

)
Note: Conditional mutant. Double labeled: green - Calb1; red - Fabp7 (assay MGI:7434455).

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Purkinje cell	Present		S4D , S4D'

S6C ©

Specimen S6C: postnatal day 8 (more

)
Note: Double labeled: green - Calb1; red - Gfap (assay MGI:7434589).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Purkinje cell	Present		S6C

S6D ©

Specimen S6D: postnatal day 8; Tg(Gfap-cre)77.6Mvs/0, Wwtr1^tm1.1Eno/Wwtr1^tm1.1Eno, Yap1^tm1.1Eno/Yap1^tm1.1Eno (more

)
Note: Conditional mutant. Double labeled: green - Calb1; red - Gfap (assay MGI:7434589).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Purkinje cell	Present		S6D	Similar as in the wild-type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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