Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:331484
Yamada S, et al., Identification and expression profile of novel STAND gene Nwd2 in the mouse central nervous system. Gene Expr Patterns. 2022 Dec;46:119284
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7450821
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Gene symbol:
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Elavl3
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Gene name:
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ELAV like RNA binding protein 3
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Antibody:
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Anti-HuC/HuD (16A11)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Assay notes:
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The antibody recognizes both Elavl3 and Elavl4.
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|
Results
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Specimen
5A:
embryonic day 14.5
(more )
Note: Double labeled: green - Elavl3 and Elavl4; red - Nwd2 (assay MGI:7450905).
Specimen
5A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Elavl3 and Elavl4; red - Nwd2 (assay MGI:7450905).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS22: habenula |
neuron |
Present |
|
5F
,
5A
,
5F'
,
5C
,
5D
|
Elavl3/4 staining as a marker for immature and differentiated neurons. Co-labeling with Nwd2 mRNA was detected in all Elavl3/4-immunopositive cells in the embryonic habenula. Almost all Nwd2-positive cells were positive for Elavl3/4.
|
Specimen
5G:
postnatal day 14
(more )
Note: Double labeled: green - Elavl3 and Elavl4; red - Nwd2 (assay MGI:7450905).
Specimen
5G:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 14
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Elavl3 and Elavl4; red - Nwd2 (assay MGI:7450905).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: medial habenular nucleus |
neuron |
Present |
|
5L
,
5G
,
5I
,
5J
,
5L'
|
Expression was detected in the medial habenula. Co-labeling with Nwd2 was mainly confined to the medial habenula.
|
TS28: lateral habenular nucleus |
neuron |
Present |
|
5J
,
5L
,
5G
,
5L''
,
5I
|
Expression was detected in the lateral habenula. Co-labeling with Nwd2 was observed in a few scattered neurons among numerous immunstained neurons. No Nwd2-positive;Elavl3/4-negative cells were seen in lateral habenula.
|
Specimen
5M:
postnatal adult
(more )
Note: Double labeled: green - Elavl3 and Elavl4; red - Nwd2 (assay MGI:7450905).
Specimen
5M:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Elavl3 and Elavl4; red - Nwd2 (assay MGI:7450905).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: medial habenular nucleus |
neuron |
Present |
|
5O
,
5P
,
5M
,
5R'
,
5R
|
Expression was detected in the medial habenula. Co-labeling with Nwd2 was mainly confined to the medial habenula.
|
TS28: lateral habenular nucleus |
neuron |
Present |
|
5R''
,
5O
,
5M
,
5P
,
5R
|
Expression was detected in the lateral habenula. Co-labeling with Nwd2 was observed in a few scattered neurons among numerous immunstained neurons. No Nwd2-positive;Elavl3/4-negative cells were seen in lateral habenula.
|
|