About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:319356 Dong K, et al., CARMN Is an Evolutionarily Conserved Smooth Muscle Cell-Specific LncRNA That Maintains Contractile Phenotype by Binding Myocardin. Circulation. 2021 Dec 7;144(23):1856-1875
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7470833
Gene symbol: Carmn
Gene name: cardiac mesoderm enhancer-associated non-coding RNA
Reporter: GFP
Direct Detection
Results
Specimen 3E GW GFP heart and aorta: postnatal adult; Carmntm1.1Cya/Carmn+, Tg(CMV-cre)1Cgn/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: heart Present 3E GW GFP heart and aorta
TS28: thoracic aorta Present 3E GW GFP heart and aorta
Specimen 3E GW GFP heart: postnatal adult; Carmntm1.1Cya/Carmn+, Tg(CMV-cre)1Cgn/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: coronary artery Present 3E GW GFP heart Specific expression in coronary arteries.
Specimen 3F GW GFP: postnatal adult; Carmntm1.1Cya/Carmn+, Tg(CMV-cre)1Cgn/0 (more )
Note: Conditional mutant. Triple labeled: green - Carmn reporter; red - Acta2 (assay MGI:7470835); white - Pecam1 (assay MGI:7470836).
Structure Level Pattern Image Note
TS28: aorta tunica media Present Regionally restricted 3F GW GFP , 3F GW Merged Expression colocalized with Acta2 in medial layer.
Specimen 3I Heart: embryonic day 16.5; Carmntm1.1Cya/Carmn+, Tg(CMV-cre)1Cgn/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS24: heart blood vessel blood vessel smooth muscle cell Present Regionally restricted 3I Heart (lower) , 3I Heart (upper) Expression in arteriolar smooth muscle cells in right veitricle.
TS24: heart right ventricle blood vessel smooth muscle cell Present Regionally restricted 3I Heart (lower) , 3I Heart (upper) Expression in arteriolar smooth muscle cells in right veitricle.
TS24: cardiac muscle tissue cardiac muscle cell Absent 3I Heart (lower) , 3I Heart (upper) No expression in cardiomyocytes.
Specimen 3I LCA: embryonic day 16.5; Carmntm1.1Cya/Carmn+, Tg(CMV-cre)1Cgn/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS24: artery tunica media blood vessel smooth muscle cell Present Regionally restricted 3I LCA (lower) , 3I LCA (upper) Expression in medial smooth muscles cells of left common carotid artery.
TS24: carotid artery blood vessel smooth muscle cell Present Regionally restricted 3I LCA (lower) , 3I LCA (upper) Expression in medial smooth muscles cells of left common carotid artery.
TS24: artery endothelium blood vessel endothelial cell Absent 3I LCA (upper) , 3I LCA (lower)
TS24: artery tunica adventitia adventitial cell Absent 3I LCA (upper) , 3I LCA (lower)
Specimen S3A GW GFP: postnatal adult; Carmntm1.1Cya/Carmn+, Tg(CMV-cre)1Cgn/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: bladder Present S3A GW GFP Expression in hollow organs such as bladder.
Specimen S3B GW GFP: postnatal adult; Carmntm1.1Cya/Carmn+, Tg(CMV-cre)1Cgn/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: brain blood vessel blood vessel smooth muscle cell Present S3B GW GFP Expression in regions of smooth muscle cells in brain.
Specimen S3C GW GFP: postnatal adult; Carmntm1.1Cya/Carmn+, Tg(CMV-cre)1Cgn/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: intestine Present S3C GW GFP Expression in hollow organs such as intestine
Specimen S3D GW GFP: postnatal adult; Carmntm1.1Cya/Carmn+, Tg(CMV-cre)1Cgn/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: gallbladder Present S3D GW GFP Expression in hollow organs such as gallbladder.
Specimen S3E GW GFP: postnatal adult; Carmntm1.1Cya/Carmn+, Tg(CMV-cre)1Cgn/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: bronchus smooth muscle cell Present S3E GW GFP Expression in regions of smooth muscle cells in bronchus.
TS28: pulmonary artery blood vessel smooth muscle cell Present S3E GW GFP Expression in regions of smooth muscle cells in pulmonary artery.
Specimen S3F GW GFP: postnatal adult; Carmntm1.1Cya/Carmn+, Tg(CMV-cre)1Cgn/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: quadriceps femoris muscle smooth muscle cell Present Regionally restricted S3F GW GFP Expression in regions of smooth muscle cells in quadriceps muscle.
Specimen S3G GW GFP: postnatal adult; Carmntm1.1Cya/Carmn+, Tg(CMV-cre)1Cgn/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: renal artery Present S3G GW GFP
TS28: ureter Present S3G GW GFP
Specimen S3H GW GFP: postnatal adult; Carmntm1.1Cya/Carmn+, Tg(CMV-cre)1Cgn/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: stomach Present S3H GW GFP Expression in hollow organs such as stomach.
Specimen S3I heart: postnatal adult; Carmntm1.1Cya/Carmn+, Tg(CMV-cre)1Cgn/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: heart blood vessel smooth muscle cell Present S3I Heart (upper) , S3I Heart (lower) Specific expression in presumptive smooth muscle cells within blood vessels.
TS28: cardiac muscle tissue cardiac muscle cell Absent S3I Heart (upper) , S3I Heart (lower) No expression in cardiomyocytes.
Specimen S3I quadriceps: postnatal adult; Carmntm1.1Cya/Carmn+, Tg(CMV-cre)1Cgn/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: quadriceps femoris muscle blood vessel smooth muscle cell Present S3I Quadriceps (upper) , S3I Quadriceps (lower) Specific expression in presumptive smooth muscle cells within blood vessels.
TS28: skeletal muscle tissue cell of skeletal muscle Absent S3I Quadriceps (lower) , S3I Quadriceps (upper) No expression in skeletal muscle cells.
Specimen S3J GW: postnatal adult; Carmntm1.1Cya/Carmn+, Tg(CMV-cre)1Cgn/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: jejunum Present Regionally restricted S3J GW GFP
TS28: small intestine smooth muscle circular layer smooth muscle cell Present S3J GW GFP Specific expression in longitudinal smooth muscle cells in outer layer and circular smooth muscle cells in inner layer.
TS28: small intestine smooth muscle longitudinal layer smooth muscle cell Present S3J GW GFP Specific expression in longitudinal smooth muscle cells in outer layer and circular smooth muscle cells in inner layer.
Specimen S3K GW: postnatal adult; Carmntm1.1Cya/Carmn+, Tg(CMV-cre)1Cgn/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: colon Present Regionally restricted S3K GW GFP
TS28: large intestine smooth muscle circular layer smooth muscle cell Present S3K GW GFP Specific expression in longitudinal smooth muscle cells in outer layer and circular smooth muscle cells in inner layer.
TS28: large intestine smooth muscle longitudinal layer smooth muscle cell Present S3K GW GFP Specific expression in longitudinal smooth muscle cells in outer layer and circular smooth muscle cells in inner layer.
Specimen S4Ci GFP: embryonic day 11.5; Carmntm1.1Cya/Carmn+, Tg(CMV-cre)1Cgn/0 (more )
Note: Conditional mutant. Double labeled: green - Carmn reporter; red - Acta2 (assay MGI:7470835).
Structure Level Pattern Image Note
TS19: dorsal aorta Present Regionally restricted S4Ci merged , S4Ci GFP Expression colocalized with Acta2 in dorsal aorta.
Specimen S4Cii GFP: embryonic day 11.5; Carmntm1.1Cya/Carmn+, Tg(CMV-cre)1Cgn/0 (more )
Note: Conditional mutant. Double labeled: green - Carmn reporter; red - Tnnt2 (assay MGI:7470837).
Structure Cell Type Level Pattern Image Note
TS19: left ventricle cardiac muscle cardiac muscle cell Present S4Cii merged , S4Cii GFP Expression slightly higher in left ventricle than in right ventricle.
Specimen S4Ciii GFP: embryonic day 11.5; Carmntm1.1Cya/Carmn+, Tg(CMV-cre)1Cgn/0 (more )
Note: Conditional mutant. Double labeled: green - Carmn reporter; red - Tnnt2 (assay MGI:7470837).
Structure Cell Type Level Pattern Image Note
TS19: right ventricle cardiac muscle cardiac muscle cell Present S4Ciii GFP , S4Ciii merged Expression slightly higher in left ventricle than in right ventricle.
Specimen S4Ei GFP: embryonic day 13.5; Carmntm1.1Cya/Carmn+, Tg(CMV-cre)1Cgn/0 (more )
Note: Conditional mutant. Double labeled: green - Carmn reporter; red - Acta2 (assay MGI:7470835).
Structure Level Pattern Image Note
TS21: carotid artery Present Regionally restricted S4Ei merged , S4Ei GFP Expression colocalized with Acta2 in left carotid artery.
Specimen S4Eii GFP: embryonic day 13.5; Carmntm1.1Cya/Carmn+, Tg(CMV-cre)1Cgn/0 (more )
Note: Conditional mutant. Double labeled: green - Carmn reporter; red - Tnnt2 (assay MGI:7470837).
Structure Cell Type Level Pattern Image Note
TS21: left ventricle cardiac muscle cardiac muscle cell Present S4Eii GFP , S4Eii merged Declining trend in expression from E11.5 to E13.5.
Specimen S4Eiii GFP: embryonic day 13.5; Carmntm1.1Cya/Carmn+, Tg(CMV-cre)1Cgn/0 (more )
Note: Conditional mutant. Double labeled: green - Carmn reporter; red - Tnnt2 (assay MGI:7470837).
Structure Cell Type Level Pattern Image Note
TS21: right ventricle cardiac muscle cardiac muscle cell Present S4Eiii GFP , S4Eiii merged Declining trend in expression from E11.5 to E13.5.
Specimen S4Gi GFP: embryonic day 16.5; Carmntm1.1Cya/Carmn+, Tg(CMV-cre)1Cgn/0 (more )
Note: Conditional mutant. Double labeled: green - Carmn reporter; red - Acta2 (assay MGI:7470835).
Structure Cell Type Level Pattern Image Note
TS24: carotid artery blood vessel smooth muscle cell Present Regionally restricted S4Gi merged , S4Gi GFP Expression restricted to smooth muscle cells in left carotid artery.
Specimen S4Gii GFP (left): embryonic day 16.5; Carmntm1.1Cya/Carmn+, Tg(CMV-cre)1Cgn/0 (more )
Note: Conditional mutant. Double labeled: green - Carmn reporter; red - Tnnt2 (assay MGI:7470837).
Structure Cell Type Level Pattern Image Note
TS24: heart blood vessel blood vessel endothelial cell Present Regionally restricted S4Gii GFP (left) , S4Gii merged (left) Expression in arteriolar smooth muscle cells.
TS24: heart endothelium blood vessel endothelial cell Absent S4Gii merged (left) , S4Gii GFP (left)
TS24: right ventricle cardiac muscle cardiac muscle cell Absent S4Gii merged (left) , S4Gii GFP (left)
Specimen S4Gii GFP (right): embryonic day 16.5; Carmntm1.1Cya/Carmn+, Tg(CMV-cre)1Cgn/0 (more )
Note: Conditional mutant. Double labeled: green - Carmn reporter; red - Acta2 (assay MGI:7470835).
Structure Cell Type Level Pattern Image Note
TS24: heart blood vessel blood vessel smooth muscle cell Present Regionally restricted S4Gii GFP (right) , S4Gii merged (right) Expression in arteriolar smooth muscle cells.
TS24: heart endothelium blood vessel endothelial cell Absent S4Gii merged (right) , S4Gii GFP (right)
TS24: right ventricle cardiac muscle cardiac muscle cell Absent S4Gii GFP (right) , S4Gii merged (right)
Specimen S4Giii GFP (left): embryonic day 16.5; Carmntm1.1Cya/Carmn+, Tg(CMV-cre)1Cgn/0 (more )
Note: Conditional mutant. Double labeled: green - Carmn reporter; red - Tnnt2 (assay MGI:7470837).
Structure Cell Type Level Pattern Image Note
TS24: heart blood vessel blood vessel smooth muscle cell Present Regionally restricted S4Giii merged (left) , S4Giii GFP (left) Expression in arteriolar smooth muscle cells.
TS24: heart endothelium blood vessel endothelial cell Absent S4Giii merged (left) , S4Giii GFP (left)
TS24: left ventricle cardiac muscle cardiac muscle cell Absent S4Giii GFP (left) , S4Giii merged (left)
Specimen S4Giii GFP (right): embryonic day 16.5; Carmntm1.1Cya/Carmn+, Tg(CMV-cre)1Cgn/0 (more )
Note: Conditional mutant. Double labeled: green - Carmn reporter; red - Acta2 (assay MGI:7470835).
Structure Cell Type Level Pattern Image Note
TS24: heart blood vessel blood vessel smooth muscle cell Present Regionally restricted S4Giii GFP (right) , S4Giii merged (right) Expression in arteriolar smooth muscle cells.
TS24: heart endothelium blood vessel endothelial cell Absent S4Giii merged (right) , S4Giii GFP (right)
TS24: right ventricle cardiac muscle cardiac muscle cell Absent S4Giii merged (right) , S4Giii GFP (right)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
09/17/2024
MGI 6.24 		The Jackson Laboratory