Gene Expression Data
Assay Details
Assay
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Reference:
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J:191149
Alves CH, et al., Loss of CRB2 in the mouse retina mimics human retinitis pigmentosa due to mutations in the CRB1 gene. Hum Mol Genet. 2013 Jan 1;22(1):35-50
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7507063
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Gene symbol:
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Patj
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Gene name:
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PATJ, crumbs cell polarity complex component
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Antibody:
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Anti-Patj
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Detection system:
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Secondary antibody coupled to Cy3
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Results
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Specimen
8C:
postnatal day 6; Crb2tm1.1Wij/?
(more )
Note: Controls could be Crb2F/F or Crb2F/+/Chx10Cre+/-. Double labeled: red - Patj; green - Mki67 (assay MGI:7507060).
Specimen
8C:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * 129S4/SvJaeSor
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Mutant Allele(s):
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Crb2tm1.1Wij/?
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Age:
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postnatal day 6
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Controls could be Crb2F/F or Crb2F/+/Chx10Cre+/-. Double labeled: red - Patj; green - Mki67 (assay MGI:7507060).
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Structure |
Level |
Pattern |
Image |
Note |
TS28: retina |
Present |
Regionally restricted |
8C
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Expression was detected in the apical region.
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Specimen
8D:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6 * SJL
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Mutant Allele(s):
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Crb2tm1.1Wij/Crb2tm1.1Wij Tg(Chx10-EGFP/cre,-ALPP)2Clc/0
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Age:
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postnatal day 6
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Conditional mutant. Double labeled: red - Patj; green - Mki67 (assay MGI:7507060).
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Structure |
Level |
Pattern |
Image |
Note |
TS28: retina |
Present |
Regionally restricted |
8D
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Immunopositive ectopic cells co-stained with Mki67-positive cells at the periphery of the developing retina.
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Specimen
S1L:
embryonic day 12.5; Crb2tm1.1Wij/?
(more )
Note: Controls could be Crb2F/F or Crb2F/+/Chx10Cre+/-.
Specimen
S1L:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * 129S4/SvJaeSor
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Mutant Allele(s):
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Crb2tm1.1Wij/?
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Controls could be Crb2F/F or Crb2F/+/Chx10Cre+/-.
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Structure |
Level |
Pattern |
Image |
Note |
TS20: retina |
Present |
Regionally restricted |
S1L
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Expression was detected in the subapical region.
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Specimen
S1O:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6 * SJL
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Mutant Allele(s):
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Crb2tm1.1Wij/Crb2tm1.1Wij Tg(Chx10-EGFP/cre,-ALPP)2Clc/0
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Conditional mutant.
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Structure |
Level |
Pattern |
Image |
Note |
TS20: retina |
Present |
Regionally restricted |
S1O
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Expression was detected in the subapical region and the cytoplasm of some cells, thought to be mitotic cells, located at the outer limiting membrane.
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Specimen
S2A:
postnatal day 3; Crb2tm1.1Wij/?
(more )
Note: Controls could be Crb2F/F or Crb2F/+/Chx10Cre+/-. Double labeled: red - Patj; green - Mpdz (assay MGI:7506785).
Specimen
S2A:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * 129S4/SvJaeSor
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Mutant Allele(s):
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Crb2tm1.1Wij/?
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Age:
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postnatal day 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Controls could be Crb2F/F or Crb2F/+/Chx10Cre+/-. Double labeled: red - Patj; green - Mpdz (assay MGI:7506785).
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Structure |
Level |
Pattern |
Image |
Note |
TS27: retina |
Present |
Regionally restricted |
S2C
,
S2A
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Immunostaining in the subapical region co-stained with Mpdz. Immunostaing was also localized in the apical region.
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Specimen
S2D:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6 * SJL
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Mutant Allele(s):
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Crb2tm1.1Wij/Crb2tm1.1Wij Tg(Chx10-EGFP/cre,-ALPP)2Clc/0
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Age:
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postnatal day 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Conditional mutant. Double labeled: red - Patj; green - Mpdz (assay MGI:7506785).
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Structure |
Level |
Pattern |
Image |
Note |
TS27: neural retina |
Present |
Regionally restricted |
S2D
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S2F
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In the cKO, close to the periphery, immunopositive ectopic cells were found. as well as ectopic immunolabeled cells that co-stained Mpdz.
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