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Gene Expression Data
Assay Details
Assay
Reference: J:110095 Ligon KL, et al., Development of NG2 neural progenitor cells requires Olig gene function. Proc Natl Acad Sci U S A. 2006 May 16;103(20):7853-8
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7547378
Gene symbol: Olig2
Gene name: oligodendrocyte transcription factor 2
Antibody: anti-OLIG2 (DF308)
Detection system: Secondary antibody coupled to Cy2
Results
Specimen 1A cx: embryonic day 18.5 (more )
Note: Double labeled: red - Olig2; green - Cspg4 (assay MGI:7547377).
Structure Level Pattern Image Note
TS26: neocortex Present 1A upper left Coexpressed with Cspg2/NG2 in neural cells with synantocyte and progenitor morphology. Only 18% of the overall Olig2+ cell population on average expressed Cspg4/NG2.

Specimen 1A vfb: embryonic day 18.5 (more )
Note: Double labeled: red - Olig2; green - Cspg4 (assay MGI:7547377).
Structure Level Pattern Image Note
TS26: forebrain Present Regionally restricted 1A upper right Expressed in the ventral forebrain. Coexpressed with Cspg2/NG2 in neural cells with synantocyte and progenitor morphology. Only 18% of the overall Olig2+ cell population on average expressed Cspg4/NG2.

Specimen 1A svz: embryonic day 18.5 (more )
Note: Double labeled: red - Olig2; green - Cspg4 (assay MGI:7547377).
Structure Level Pattern Image Note
TS26: cerebral cortex subventricular zone Present 1A lower left Olig2+ cells rarely expressed Cspg4/NG2. Only 8% of the Olig2+ cell population expressed Cspg4/NG2.

Specimen 1A iz: embryonic day 18.5 (more )
Note: Double labeled: red - Olig2; green - Cspg4 (assay MGI:7547377).
Structure Level Pattern Image Note
TS26: brain white matter Present 1A lower right Coexpressed with Cspg2/NG2 in neural cells with synantocyte and progenitor morphology. Only 53% of the Olig2+ cell population expressed Cspg4/NG2.

Specimen 1C +/-: embryonic day 18.5; Olig2tm1Rth/Olig2+ (more )
Note: Double labeled: red - Olig2 (rabbit antibody); green - Olig2 (mouse antibody; assay MGI:7547380).
Structure Level Pattern Image Note
TS26: spinal cord white matter Present 1C upper right , 1C upper left Precise colocalization of signal from two different antibodies.

Specimen 1C -/-: embryonic day 18.5; Olig2tm1Rth/Olig2tm1Rth (more )
Note: Double labeled: red - Olig2 (rabbit antibody); green - Olig2 (mouse antibody; assay MGI:7547380).
Structure Level Pattern Image Note
TS26: spinal cord white matter Absent 1C lower right , 1C lower left

Specimen not shown: embryonic day 18.5; Olig2tm1Rth/Olig2+ (more )
Structure Level Pattern Image Note
TS26: brain Present Precise colocalization of signal from two different antibodies.

Specimen not shown: postnatal adult; Olig2tm1Rth/Olig2+ (more )
Structure Level Pattern Image Note
TS28: brain Present Precise colocalization of signal from two different antibodies.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
06/12/2024
MGI 6.13
The Jackson Laboratory