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Gene Expression Data
Assay Details
Assay
Reference: J:240101 Lv J, et al., 5-hydroxytryptamine synthesized in the aorta-gonad-mesonephros regulates hematopoietic stem and progenitor cell survival. J Exp Med. 2017 Feb;214(2):529-545
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7549026
Gene symbol: Pecam1
Gene name: platelet/endothelial cell adhesion molecule 1
Antibody: anti-CD31
Detection system: fluorescein
Results
Specimen 2B E10.5 tph2+/+: embryonic day 10.5 (more )
Note: Double labeled: green - Pecam1; red - Runx1 (assay MGI:7549025).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta aortic endothelial cell Present 2B E10.5 tph2+/+
Specimen 2B E10.5 tph2+/-: embryonic day 10.5; Tph2tm1.1Zfc/Tph2+ (more )
Note: Double labeled: green - Pecam1; red - Runx1 (assay MGI:7549025).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta aortic endothelial cell Present 2B E10.5 tph2+/-
Specimen 2B E11.0 tph2+/+: embryonic day 11.0 (more )
Note: Double labeled: green - Pecam1; red - Runx1 (assay MGI:7549025).
Structure Cell Type Level Pattern Image Note
TS18: dorsal aorta aortic endothelial cell Present 2B E11.0 tph2 +/+
Specimen 2B E11.0 tph2+/-: embryonic day 11.0; Tph2tm1.1Zfc/Tph2+ (more )
Note: Double labeled: green - Pecam1; red - Runx1 (assay MGI:7549025).
Structure Cell Type Level Pattern Image Note
TS18: dorsal aorta aortic endothelial cell Present 2B E11.0 tph2+/-
Specimen 2H pet-cre; tph2+/+: embryonic day 10.5; Tg(Fev-cre)1Esd/0 (more )
Note: Triple labeled: green - Pecam1; white - Cd34 (assay MGI:7549027); red - Runx1 (assay MGI:7549025).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta aortic endothelial cell Present 2H pet1-cre; tph2+/+
Specimen 2H pet-cre; tph2+/fl: embryonic day 10.5; Tg(Fev-cre)1Esd/0, Tph2tm1.1Kpl/Tph2+ (more )
Note: Conditional mutant. Triple labeled: green - Pecam1; white - Cd34 (assay MGI:7549027); red - Runx1 (assay MGI:7549025).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta aortic endothelial cell Present 2H pet1-cre; tph2+/fl
Specimen 2J E10.5 tph2+/fl: embryonic day 10.5; Tph2tm1.1Kpl/Tph2+ (more )
Note: Double labeled: green - Pecam1; red - Runx1 (assay MGI:7549025).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta aortic endothelial cell Present 2J E10.5 tph2+/fl
Specimen 2J E10.5 vec-cre; tph2+/fl: embryonic day 10.5; Tg(Cdh5-cre)1Spe/0, Tph2tm1.1Kpl/Tph2+ (more )
Note: Conditional mutant. Double labeled: green - Pecam1; red - Runx1 (assay MGI:7549025).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta aortic endothelial cell Present 2J E10.5 vec-cre; tph2+/fl
Specimen 2J E11,0 tph2+/fl: embryonic day 11.0; Tph2tm1.1Kpl/Tph2+ (more )
Note: Double labeled: green - Pecam1; red - Runx1 (assay MGI:7549025).
Structure Cell Type Level Pattern Image Note
TS18: dorsal aorta aortic endothelial cell Present 2J E11.0 tph2+/fl
Specimen 2J E11,0 vec-cre; tph2+/fl: embryonic day 11.0; Tg(Cdh5-cre)1Spe/0, Tph2tm1.1Kpl/Tph2+ (more )
Note: Conditional mutant. Double labeled: green - Pecam1; red - Runx1 (assay MGI:7549025).
Structure Cell Type Level Pattern Image Note
TS18: dorsal aorta aortic endothelial cell Present 2J E11.0 vec-cre; tph2+/fl
Specimen 3C tph2+/+: embryonic day 10.5 (more )
Note: Triple labeled: green - Pecam1; red - Casp3 (assay MGI:7549028); white - Cd34 (assay MGI:7549027).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta aortic endothelial cell Present 3C tph2+/+ (merged) , 3C tph2+/+ CD31
Specimen 3C tph2+/-: embryonic day 10.5; Tph2tm1.1Zfc/Tph2+ (more )
Note: Triple labeled: green - Pecam1; red - Casp3 (assay MGI:7549028); white - Cd34 (assay MGI:7549027).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta aortic endothelial cell Present 3C tph2+/- CD31 , 3C tph2+/- (merged)
Specimen 3D tph2+/+: embryonic day 10.5 (more )
Note: Triple labeled: green - Pecam1; white - Runx1 (assay MGI:7549025); red - TUNEL.
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta aortic endothelial cell Present 3D tph2+/+ (merged) , 3D tph2+/+ CD31
Specimen 3D tph2+/-: embryonic day 10.5; Tph2tm1.1Zfc/Tph2+ (more )
Note: Triple labeled: green - Pecam1; white - Runx1 (assay MGI:7549025); red - TUNEL.
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta aortic endothelial cell Present 3D tph2+/- (merged) , 3D tph2+/- CD31
Specimen 3E pet-cre; tph2+/+: embryonic day 10.5; Tg(Fev-cre)1Esd/0 (more )
Note: Triple labeled: green - Pecam1; red - Casp3 (assay MGI:7549028); white - Cd34 (assay MGI:7549027).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta aortic endothelial cell Present 3E pet1-cre; tph2+/+ (merged) , 3E pet1-cre; tph2+/+ CD31
Specimen 3E pet-cre; tph2+/fl: embryonic day 10.5; Tg(Fev-cre)1Esd/0, Tph2tm1.1Kpl/Tph2+ (more )
Note: Conditional mutant. Triple labeled: green - Pecam1; red - Casp3 (assay MGI:7549028); white - Cd34 (assay MGI:7549027).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta aortic endothelial cell Present 3E pet1-cre; tph2+/fl CD31 , 3E pet1-cre; tph2+/fl (merged)
Specimen 3F tph2+/fl: embryonic day 10.5; Tph2tm1.1Kpl/Tph2+ (more )
Note: Double labeled: green - Pecam1; red - Casp3 (assay MGI:7549028).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta aortic endothelial cell Present 3F tph2+/fl
Specimen 3F vec-cre; tph2+/fl: embryonic day 10.5; Tg(Cdh5-cre)1Spe/0, Tph2tm1.1Kpl/Tph2+ (more )
Note: Conditional mutant. Double labeled: green - Pecam1; red - Casp3 (assay MGI:7549028).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta aortic endothelial cell Present 3F vec-cre; tph2+/fl
Specimen 3G tph2+/+: embryonic day 10.5 (more )
Note: Double labeled: green - Pecam1; red - Akt1 (assay MGI:7549030).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta aortic endothelial cell Present 3G tph2+/+
Specimen 3G tph2+/-: embryonic day 10.5; Tph2tm1.1Zfc/Tph2+ (more )
Note: Double labeled: green - Pecam1; red - Akt1 (assay MGI:7549030).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta aortic endothelial cell Present 3G tph2+/-
Specimen 3N tph2+/+ (upper): embryonic day 10.5 (more )
Note: Double labeled: green - Pecam1; red - Foxo1 (assay MGI:7549031).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta aortic endothelial cell Present 3N tph2+/+ (upper)
Specimen 3N tph2+/+ (lower)): embryonic day 10.5 (more )
Note: Double labeled: green - Pecam1; red - Foxo1 (assay MGI:7549033).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta aortic endothelial cell Present 3N tph2+/+ (lower)
Specimen 3N tph2+/- (upper): embryonic day 10.5; Tph2tm1.1Zfc/Tph2+ (more )
Note: Double labeled: green - Pecam1; red - Foxo1 (assay MGI:7549031).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta aortic endothelial cell Present 3N tph2+/- (upper)
Specimen 3N tph2+/- (lower): embryonic day 10.5; Tph2tm1.1Zfc/Tph2+ (more )
Note: Double labeled: green - Pecam1; red - Foxo1 (assay MGI:7549033).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta aortic endothelial cell Present 3N tph2+/- (lower)
Specimen 4B E10.5 AGM: embryonic day 10.5 (more )
Note: Double labeled: green - Pecam1; red - Fev (assay MGI:7549035).
Structure Cell Type Level Pattern Image Note
TS17: aorta-gonad-mesonephros aortic endothelial cell Present 4B E10.5 AGM
Specimen 4B E11.0 AGM: embryonic day 11.0 (more )
Note: Double labeled: green - Pecam1; red - Fev (assay MGI:7549035).
Structure Cell Type Level Pattern Image Note
TS18: aorta-gonad-mesonephros aortic endothelial cell Present 4B E11.0 AGM
Specimen 4B E11.5 AGM: embryonic day 11.5 (more )
Note: Double labeled: green - Pecam1; red - Fev (assay MGI:7549035).
Structure Cell Type Level Pattern Image Note
TS19: aorta-gonad-mesonephros aortic endothelial cell Present 4B E11.5 AGM
Specimen 4B E12.5 FL: embryonic day 12.5 (more )
Note: Triple labeled: green - Pecam1; white - Cd34 (assay MGI:7549027); red - Fev (assay MGI:7549035).
Structure Cell Type Level Pattern Image Note
TS20: liver fetal liver hematopoietic progenitor cell Present 4B E12.5 FL
Specimen 4D E10.5: embryonic day 10.5 (more )
Note: Double labeled: green - Pecam1; red - Tph1 (assay MGI:7549036).
Structure Cell Type Level Pattern Image Note
TS17: aorta-gonad-mesonephros aortic endothelial cell Present 4D E10.5
Specimen 4D E11.0: embryonic day 11.0 (more )
Note: Double labeled: green - Pecam1; red - Tph1 (assay MGI:7549036).
Structure Cell Type Level Pattern Image Note
TS18: aorta-gonad-mesonephros aortic endothelial cell Present 4D E11.0
Specimen 4F E10.5: embryonic day 10.5 (more )
Note: Double labeled: green - Pecam1; red - Tph2 (assay MGI:7549037).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta aortic endothelial cell Present 4F E10.5
Specimen 4F E11.5: embryonic day 11.5 (more )
Note: Double labeled: green - Pecam1; red - Tph2 (assay MGI:7549037).
Structure Cell Type Level Pattern Image Note
TS19: dorsal aorta aortic endothelial cell Present 4F E11.5
Specimen 4J E10.5: embryonic day 10.5 (more )
Note: Triple labeled: green - Pecam1; red - Ddc (assay MGI:7549038); white - Tph2 (assay MGI:7549037).
Structure Cell Type Level Pattern Image Note
TS17: aorta-gonad-mesonephros aortic endothelial cell Present 4J E10.5 CD31 , 4J E10.5 (merged)
Specimen 4J E11.0: embryonic day 11.0 (more )
Note: Triple labeled: green - Pecam1; red - Ddc (assay MGI:7549038); white - Tph2 (assay MGI:7549037).
Structure Cell Type Level Pattern Image Note
TS18: aorta-gonad-mesonephros aortic endothelial cell Present 4J E11.5 (merged) , 4J E11.5 CD31
Specimen 4K E10.5: embryonic day 10.5 (more )
Note: Double labeled: green - Pecam1; red - 5-HT.
Structure Cell Type Level Pattern Image Note
TS17: aorta-gonad-mesonephros aortic endothelial cell Present 4K E10.5 (upper) , 4K E10.5 (lower)
Specimen 4K E11.0: embryonic day 11.0 (more )
Note: Double labeled: green - Pecam1; red - 5-HT.
Structure Cell Type Level Pattern Image Note
TS18: aorta-gonad-mesonephros aortic endothelial cell Present 4K E11.0 (lower) , 4K E11.0 (upper)
Specimen 4M tph2+/+: embryonic day 10.5 (more )
Note: Double labeled: green - Pecam1; red - 5-HT.
Structure Cell Type Level Pattern Image Note
TS17: aorta-gonad-mesonephros aortic endothelial cell Present 4M tph2+/+ (upper) , 4M tph2+/+ (lower)
Specimen 4M tph2+/-: embryonic day 10.5; Tph2tm1.1Zfc/Tph2+ (more )
Note: Double labeled: green - Pecam1; red - 5-HT.
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta aortic endothelial cell Present 4M tph2+/- (upper) , 4M tph2+/- (lower)
Specimen 4M tph2-/-: embryonic day 10.5; Tph2tm1.1Zfc/Tph2tm1.1Zfc (more )
Note: Double labeled: green - Pecam1; red - 5-HT.
Structure Cell Type Level Pattern Image Note
TS17: aorta-gonad-mesonephros aortic endothelial cell Present 4M tph2-/- (upper) , 4M tph2-/- (lower)
Specimen 4N pet-cre; tph2+/+: embryonic day 10.5; Tg(Fev-cre)1Esd/0 (more )
Note: Double labeled: green - Pecam1; red - 5-HT.
Structure Cell Type Level Pattern Image Note
TS17: aorta-gonad-mesonephros aortic endothelial cell Present 4N pet1-cre; tph2+/+
Specimen 4N pet-cre; tph2+/fl: embryonic day 10.5; Tg(Fev-cre)1Esd/0, Tph2tm1.1Kpl/Tph2+ (more )
Note: Conditional mutant. Double labeled: green - Pecam1; red - 5-HT.
Structure Cell Type Level Pattern Image Note
TS17: aorta-gonad-mesonephros aortic endothelial cell Present 4N pet1-cre; tph2+/fl
Specimen 4N pet-cre; tph2fl/fl: embryonic day 10.5; Tg(Fev-cre)1Esd/0, Tph2tm1.1Kpl/Tph2tm1.1Kpl (more )
Note: Conditional mutant. Double labeled: green - Pecam1; red - 5-HT.
Structure Cell Type Level Pattern Image Note
TS17: aorta-gonad-mesonephros aortic endothelial cell Present 4N pet1-cre; tph2fl/fl
Specimen 4N tph2+/fl: embryonic day 10.5; Tph2tm1.1Kpl/Tph2+ (more )
Note: Double labeled: green - Pecam1; red - 5-HT.
Structure Cell Type Level Pattern Image Note
TS17: aorta-gonad-mesonephros aortic endothelial cell Present 4N tph2+/fl
Specimen 4N vec-cre; tph2+/fl: embryonic day 10.5; Tg(Cdh5-cre)1Spe/0, Tph2tm1.1Kpl/Tph2+ (more )
Note: Conditional mutant. Double labeled: green - Pecam1; red - 5-HT.
Structure Cell Type Level Pattern Image Note
TS17: aorta-gonad-mesonephros aortic endothelial cell Present 4N vec-cre; tph2+/fl
Specimen 4N vec-cre; tph2fl/fl: embryonic day 10.5; Tg(Cdh5-cre)1Spe/0, Tph2tm1.1Kpl/Tph2tm1.1Kpl (more )
Note: Conditional mutant. Double labeled: green - Pecam1; red - 5-HT.
Structure Cell Type Level Pattern Image Note
TS17: aorta-gonad-mesonephros aortic endothelial cell Present 4N vec-cre; tph2fl/fl
Specimen 5B E10.5 AGM: embryonic day 10.5 (more )
Note: Triple labeled: green - Pecam1; white - Cd34 (assay MGI:7549027); red - Htr5a (assay MGI:7549040).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta aortic endothelial cell Present 5B E10.5 AGM CD31 , 5B E10.5 AGM (merged)
Specimen 5B E11.5 AGM: embryonic day 11.5 (more )
Note: Triple labeled: green - Pecam1; white - Cd34 (assay MGI:7549027); red - Htr5a (assay MGI:7549040).
Structure Cell Type Level Pattern Image Note
TS19: dorsal aorta aortic endothelial cell Present 5B E11.5 AGM CD31 , 5B E11.5 AGM (merged)
Specimen 5B E11.5 FL: embryonic day 11.5 (more )
Note: Triple labeled: green - Pecam1; white - Cd34 (assay MGI:7549027); red - Htr5a (assay MGI:7549040).
Structure Cell Type Level Pattern Image Note
TS19: liver fetal liver hematopoietic progenitor cell Present 5B E11.5 FL CD31 , 5B E11.5 FL (merged)
Specimen 6A E10.5 tph2+/+: embryonic day 10.5 (more )
Note: Double labeled: green - Pecam1; red - Runx1 (assay MGI:7549025).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta aortic endothelial cell Present 6A E10.5 tph2+/+
Specimen 6A E10.5 tph2-/-: embryonic day 10.5; Tph2tm1.1Zfc/Tph2tm1.1Zfc (more )
Note: Double labeled: green - Pecam1; red - Runx1 (assay MGI:7549025).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta aortic endothelial cell Present 6A E10.5 tph2-/-
Specimen 6A E11.0 tph2+/+: embryonic day 11.0 (more )
Note: Double labeled: green - Pecam1; red - Runx1 (assay MGI:7549025).
Structure Cell Type Level Pattern Image Note
TS18: dorsal aorta aortic endothelial cell Present 6A E11.0 tph2+/+
Specimen 6A E11.0 tph2-/-: embryonic day 11.0; Tph2tm1.1Zfc/Tph2tm1.1Zfc (more )
Note: Double labeled: green - Pecam1; red - Runx1 (assay MGI:7549025).
Structure Cell Type Level Pattern Image Note
TS18: dorsal aorta aortic endothelial cell Present 6A E11.0 tph2-/-
Specimen 6D pet1-cre; tph2+/+: embryonic day 10.5; Tg(Fev-cre)1Esd/0 (more )
Note: Triple labeled: green - Pecam1; white - Cd34 (assay MGI:7549027); red - Runx1 (assay MGI:7549025).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta aortic endothelial cell Present 6D pet1-cre; tph2+/+
Specimen 6D pet1-cre; tph2fl/fl: embryonic day 10.5; Tg(Fev-cre)1Esd/0, Tph2tm1.1Kpl/Tph2tm1.1Kpl (more )
Note: Conditional mutant. Triple labeled: green - Pecam1; white - Cd34 (assay MGI:7549027); red - Runx1 (assay MGI:7549025).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta aortic endothelial cell Present 6D pet1-cre; tph2fl/fl
Specimen 6G tph2+/+: embryonic day 10.5 (more )
Note: Triple labeled: green - Pecam1; red - Casp3 (assay MGI:7549028); white - Cd34 (assay MGI:7549027).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta aortic endothelial cell Present 6G tph2+/+ (merged) , 6G tph2+/+ CD31
Specimen 6G tph2+/-: embryonic day 10.5; Tph2tm1.1Zfc/Tph2+ (more )
Note: Triple labeled: green - Pecam1; red - Casp3 (assay MGI:7549028); white - Cd34 (assay MGI:7549027).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta aortic endothelial cell Present 6G tph2+/- CD31 , 6G tph2+/- (merged)
Specimen 6G tph2-/-: embryonic day 10.5; Tg(Fev-cre)1Esd/0, Tph2tm1.1Kpl/Tph2+ (more )
Note: Conditional mutant. Triple labeled: green - Pecam1; red - Casp3 (assay MGI:7549028); white - Cd34 (assay MGI:7549027).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta aortic endothelial cell Present 6G tph2-/- (merged) , 6G tph2-/- CD31
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03/25/2025
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