Gene Expression Data
Assay Details
Assay
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Reference:
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J:217461
Yang G, et al., An eIF4E1/4E-T complex determines the genesis of neurons from precursors by translationally repressing a proneurogenic transcription program. Neuron. 2014 Nov 19;84(4):723-39
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7564072
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Gene symbol:
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Neurog2
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Gene name:
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neurogenin 2
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Antibody:
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anti-Neurogenin2
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Results
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Specimen
8E Neurogenin2:
embryonic day 12.0
(more )
Note: Triple labeled: blue - Neurog2 protein; green - Eif4enif1 (assay MGI:7564070); red - Neurog2 RNA (assay MGI:7564091).
Specimen
8E Neurogenin2:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 12.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: blue - Neurog2 protein; green - Eif4enif1 (assay MGI:7564070); red - Neurog2 RNA (assay MGI:7564091).
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Structure |
Level |
Pattern |
Image |
Note |
TS20: cerebral cortex |
Present |
Regionally restricted |
8E (left)
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Co-expression with Neurog2 mRNA in some but not all Neurog2 mRNA expressing foci.
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Specimen
8E Neurogenin2 (upper):
embryonic day 12.0
(more )
Note: Triple labeled: blue - Neurog2 protein; green - Eif4enif1 (assay MGI:7564070); red - Neurog2 RNA (assay MGI:7564091).
Specimen
8E Neurogenin2 (upper):
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 12.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: blue - Neurog2 protein; green - Eif4enif1 (assay MGI:7564070); red - Neurog2 RNA (assay MGI:7564091).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: cerebral cortex |
Present |
Regionally restricted |
8E Neurogenin2 (upper)
,
8E Merge (upper)
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Co-expression with Neurog2 mRNA in some but not all Neurog2 mRNA expressing foci.
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Specimen
8E Neurogenin2 (lower):
embryonic day 12.0
(more )
Note: Triple labeled: blue - Neurog2 protein; green - Eif4enif1 (assay MGI:7564070); red - Neurog2 RNA (assay MGI:7564091).
Specimen
8E Neurogenin2 (lower):
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 12.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
|
Triple labeled: blue - Neurog2 protein; green - Eif4enif1 (assay MGI:7564070); red - Neurog2 RNA (assay MGI:7564091).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: cerebral cortex |
Present |
Regionally restricted |
8E Merge (lower)
,
8E Neurogenin2 (lower)
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Co-expression with Neurog2 mRNA in some but not all Neurog2 mRNA expressing foci.
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