About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:84406 Hikita ST, et al., Integrin alpha4beta1 (VLA-4) expression and activity in retinal and peripheral neurons. Mol Cell Neurosci. 2003 Jul;23(3):427-39
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7564322
Gene symbol: Itga4
Gene name: integrin alpha 4
Antibody: anti-alpha 4 integrin
Detection system: Secondary antibody
Results
Specimen 1A/C: embryonic day 15.0 (more )
Note: The same pattern of staining was observed using the anti-alpha4 monoclonal antibody 9C10. Double labeled: green - Itga4; red - Tubb3 (assay MGI:7564323).
Structure Cell Type Level Pattern Image Note
TS23: retina nuclear layer retinal ganglion cell Present 1A , 1C
TS23: lens Present 1A , 1C
TS23: optic nerve Present 1A , 1C
TS23: retina nerve fiber layer Present 1A , 1C
TS23: hyaloid cavity hyalocyte Present 1C , 1A
TS23: retina nuclear layer retinoblast Strong 1C , 1A Strong expression in the peripheral tips of the retina, which contain the least differentiated cells.
TS23: vitreous humour Present Clusters 1A , 1C
Specimen 1D/F: embryonic day 15.0 (more )
Note: The same pattern of staining was observed using the anti-alpha4 monoclonal antibody 9C10. Double labeled: green - Itga4; red - Tubb3 (assay MGI:7564323).
Structure Cell Type Level Pattern Image Note
TS23: vitreous humour hyalocyte Present Clusters 1F , 1D
TS23: retina nuclear layer retinal ganglion cell Present 1F , 1D
TS23: retina nuclear layer retinoblast Present 1F , 1D
Specimen 2A/C: embryonic day 15.0-16.0 (more )
Note: Double labeled: green - Itga4; red - Itgb1 (assay MGI:7564324).
Structure Cell Type Level Pattern Image Note
TS23: retina nuclear layer retinal ganglion cell Present 2C , 2A
TS23: retina nuclear layer retinoblast Present 2C , 2A
TS23: hyaloid vascular plexus Present 2A , 2C
TS23: hyaloid cavity hyalocyte Present 2C , 2A
TS23: lens Present 2A , 2C
TS23: optic nerve Present 2A , 2C
TS23: retina nerve fiber layer Present 2A , 2C
Specimen 2D/F: embryonic day 15.0-16.0 (more )
Note: Double labeled: green - Itga4; red - Ricinus communis agglutinin I lectin.
Structure Level Pattern Image Note
TS23: hyaloid vascular plexus Present 2F , 2D Costaining with Ricinus communis agglutinin I lectin.
Specimen 3A/D: embryonic day 15.0 (more )
Note: Triple labeled: green - Itga4; red - Itgb1 (assay MGI:7564325); blue - Spp1 (assay MGI:7564326).
Structure Level Pattern Image Note
TS23: hyaloid cavity Present 3D , 3A
TS23: lens Present 3D , 3A
TS23: optic nerve Present 3D , 3A
TS23: retina nerve fiber layer Present 3A , 3D
TS23: retina nuclear layer Present 3D , 3A
Specimen 3E/H: embryonic day 15.0 (more )
Note: Triple labeled: green - Itga4; red - Itgb1 (assay MGI:7564325); blue - Spp1 (assay MGI:7564326).
Structure Cell Type Level Pattern Image Note
TS23: retina nerve fiber layer Present 3H , 3E
TS23: vitreous humour Present 3H , 3E
TS23: retina nuclear layer retinal ganglion cell Present 3H , 3E
TS23: retina nuclear layer retinoblast Present 3H , 3E
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
06/12/2024
MGI 6.13 		The Jackson Laboratory