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Gene Expression Data
Assay Details
Assay
Reference: J:346442 Migale R, et al., FOXL2 interaction with different binding partners regulates the dynamics of ovarian development. Sci Adv. 2024 Mar 22;10(12):eadl0788
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7628313
Gene symbol: Ddx4
Gene name: DEAD box helicase 4
Antibody: Anti-DDX4/MVH (ab27591)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 5A Control: postnatal day 0; Usp7tm2Wgu/Usp7tm2Wgu (more )
Note: Double labeled: green - Ddx4; red - Foxl2 (assay MGI:7628312).
Structure Level Pattern Image Note
TS27: germ cell of ovary Present Regionally restricted 5A DDX4-FOXL2 Control Expression in germ cells densely packed in cortical region.
TS27: oocyte Present Regionally restricted 5A DDX4-FOXL2 Control Expression in indvidual oocytes surrounded by granulosa cells.
Specimen 5A Sf1:Cretg/+;Usp7fl/fl: postnatal day 0; Tg(Nr5a1-cre)2Klp/0, Usp7tm2Wgu/Usp7tm2Wgu (more )
Note: Conditional mutant. Double labeled: green - Ddx4; red - Foxl2 (assay MGI:7628312).
Structure Level Pattern Image Note
TS27: oocyte Present Regionally restricted 5A DDX4-FOXL2 Sf1:Cretg/+;Usp7fl/fl Expression in oocytes mostly remaining in germ cell nests.
Specimen 5B Control: postnatal week 1; Usp7tm2Wgu/Usp7tm2Wgu (more )
Note: Double labeled: green - Ddx4; red - Foxl2 (assay MGI:7628312).
Structure Level Pattern Image Note
TS28: oocyte of primordial follicle Present Regionally restricted 5B DDX4-FOXL2 Control Expression in germ cells contained in primordial follicles in cortex.
TS28: oocyte of primary follicle Present Regionally restricted 5B DDX4-FOXL2 Control Expression in germ cells contained in primary folicles in medulla.
Specimen 5B Sf1:Cretg/+;Usp7fl/fl: postnatal week 1; Tg(Nr5a1-cre)2Klp/0, Usp7tm2Wgu/Usp7tm2Wgu (more )
Note: Conditional mutant. Double labeled: green - Ddx4; red - Foxl2 (assay MGI:7628312).
Structure Level Pattern Image Note
TS28: oocyte of primordial follicle Present 5B DDX4-FOXL2 Sf1:Cretg/+;Usp7fl/fl
TS28: germ cell of ovary Present Regionally restricted 5B DDX4-FOXL2 Sf1:Cretg/+;Usp7fl/fl Expression in germ cells in nestlike structures.
Specimen S16A Control: postnatal day 0; Usp7tm2Wgu/Usp7tm2Wgu (more )
Note: Double labeled: green - Ddx4; red - Sox9 (assay MGI:7628315).
Structure Level Pattern Image Note
TS27: germ cell of testis Present S16A Control (lower)
Specimen S16A Sf1:Cretg/+;Usp7fl/fl: postnatal day 0; Tg(Nr5a1-cre)2Klp/0, Usp7tm2Wgu/Usp7tm2Wgu (more )
Note: Conditional mutant. Double labeled: green - Ddx4; red - Sox9 (assay MGI:7628315).
Structure Level Pattern Image Note
TS27: germ cell of testis Present S16A Sf1:Cretg/+;Usp7fl/fl (lower) Expression in increased number of germ cells compared to control.
Specimen S16B Control: postnatal day 7; Usp7tm2Wgu/Usp7tm2Wgu (more )
Note: Double labeled: green - Ddx4; red - Sox9 (assay MGI:7628315).
Structure Level Pattern Image Note
TS28: germ cell of testis Present S16B Control DDX4 , S16B Control DDX4/SOX9
Specimen S16B Sf1:Cretg/+;Usp7fl/fl: postnatal day 7; Tg(Nr5a1-cre)2Klp/0, Usp7tm2Wgu/Usp7tm2Wgu (more )
Note: Conditional mutant. Double labeled: green - Ddx4; red - Sox9 (assay MGI:7628315).
Structure Level Pattern Image Note
TS28: germ cell of testis Present S16B Sf1:Cretg/+;Usp7fl/fl DDX4/SOX9 , S16B Sf1:Cretg/+;Usp7fl/fl DDX4 Expression in increased number of germ cells compared to control.
Specimen S16D Control: postnatal week 8; Usp7tm2Wgu/Usp7tm2Wgu (more )
Note: Double labeled: green - Ddx4; red - Sox9 (assay MGI:7628315).
Structure Level Pattern Image Note
TS28: spermatid Present S16D Control (upper) , S16D Control (lower)
Specimen S16D Sf1:Cretg/+;Usp7fl/fl: postnatal week 8; Tg(Nr5a1-cre)2Klp/0, Usp7tm2Wgu/Usp7tm2Wgu (more )
Note: Conditional mutant. Double labeled: green - Ddx4; red - Sox9 (assay MGI:7628315).
Structure Level Pattern Image Note
TS28: spermatid Absent S16D Sf1:Cretg/+;Usp7fl/fl (lower) , S16D Sf1:Cretg/+;Usp7fl/fl (upper)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory