Gene Expression Data
Assay Details
Assay
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Reference:
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J:44290
Shibata T, et al., Glutamate transporter GLAST is expressed in the radial glia-astrocyte lineage of developing mouse spinal cord. J Neurosci. 1997 Dec 1;17(23):9212-9
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7643802
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Gene symbol:
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Nes
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Gene name:
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nestin
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Antibody:
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anti-nestin
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Detection system:
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Secondary antibody coupled to Texas Red
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Results
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Specimen
6B:
embryonic day 13.5
(more )
Note: Double labeled: red - Nes; green - Slc1a3 (assay MGI:7643801).
Specimen
6B:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Nes; green - Slc1a3 (assay MGI:7643801).
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS21: spinal cord marginal layer |
spinal cord radial glial cell |
Present |
Regionally restricted |
6B
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Expression inside endfoot of radial processes.
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Specimen
6C:
embryonic day 15.5
(more )
Note: Double labeled: red - Nes; green - Slc1a3 (assay MGI:7643801).
Specimen
6C:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Nes; green - Slc1a3 (assay MGI:7643801).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS23: spinal cord mantle layer |
spinal cord radial glial cell |
Present |
Regionally restricted |
6C
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Expression in shafts of radial processes.
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Specimen
6D:
embryonic day 15.5
(more )
Note: Double labeled: red - Nes; green - Slc1a3 (assay MGI:7643801).
Specimen
6D:
(close )
Genetic Background:
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C57BL/6J
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Age:
|
embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
|
Bouin's Fixative
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Embedding:
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Paraffin
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Note:
|
Double labeled: red - Nes; green - Slc1a3 (assay MGI:7643801).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS23: spinal cord marginal layer |
spinal cord radial glial cell |
Present |
Regionally restricted |
6D
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Expression in shafts of radial processes.
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