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Gene Expression Data
Assay Details
Assay
Reference: J:84137 Wu D, et al., Neuronal lineage-specific induction of phospholipase Cepsilon expression in the developing mouse brain. Eur J Neurosci. 2003 Apr;17(8):1571-80
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7645213
Gene symbol: Nes
Gene name: nestin
Antibody: Anti-Nestin clone 25 (611659)
Detection system: Secondary antibody coupled to rhodamine
Results
Specimen 3Bb: embryonic day 10.5 (more )
Note: Fix - 3.7% paraformaldehyde. Double labeled: red - Nes; green - Plce1 (assay MGI:7645206).
Structure Cell Type Level Pattern Image Note
TS17: hindbrain neural stem cell Present 3Bc , 3Be , 3Bf , 3Bb
Specimen 3C: embryonic day 10.5 (more )
Note: Fix - 3.7% paraformaldehyde. Double labeled: red - Nes; green - Plce1 (assay MGI:7645206).
Structure Cell Type Level Pattern Image Note
TS17: telencephalon neural stem cell Present Non-Uniform 3C nestin , 3C merge top Nestin staining almost uniformly co-stained with Plce1, except for the outermost zone, which exhibited stronger Nestin staining.
Specimen 4Ab: embryonic day 14.5 (more )
Note: Fix - 3.7% paraformaldehyde. Double labeled: red - Nes; green - Plce1 (assay MGI:7645206).
Structure Cell Type Level Pattern Image Note
TS22: pia mater Strong 4Ac , 4Af , 4Ae , 4Ab
TS22: telencephalon ventricular layer neural stem cell Present 4Ac , 4Ab , 4Ae , 4Af Staining did not co-localize with Plce1-highly positive cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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06/12/2024
MGI 6.13 		The Jackson Laboratory