Gene Expression Data
Assay Details
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Assay
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Reference:
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J:84852
Imanaka-Yoshida K, et al., The dynamic expression of tenascin-C and tenascin-X during early heart development in the mouse. Differentiation. 2003 Jun;71(4-5):291-8
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:7645230
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Gene symbol:
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Tnc
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Gene name:
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tenascin C
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Reporter:
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lacZ
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Direct Detection
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Results
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Specimen
1A E7.5:
embryonic day 7.5; Tnctm1Sia/Tnc +
(more  )
Specimen
1A E7.5:
(close  )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Tnctm1Sia/Tnc+
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: cardiogenic plate |
Present |
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1A E7.5
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Expression in heart forming crescent in presomite stage embryo.
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Specimen
1A E8.0:
embryonic day 8.0; Tnctm1Sia/Tnc +
(more )
Specimen
1A E8.0:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Tnctm1Sia/Tnc+
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: primitive heart tube |
Present |
Regionally restricted |
1A E8.0
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When bilateral heart primordia fuse to form a single heart tube, expression limited to caudal part of heart.
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Specimen
1A E8.5:
embryonic day 8.5; Tnctm1Sia/Tnc +
(more )
Specimen
1A E8.5:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Tnctm1Sia/Tnc+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: heart |
Present |
Regionally restricted |
1A E8.5
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When bilateral heart primordia fuse to form a single heart tube, expression limited to caudal part of heart.
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Specimen
1B:
embryonic day 7.5; Tnctm1Sia/Tnc +
(more )
Specimen
1B:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Tnctm1Sia/Tnc+
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS11: intraembryonic coelom |
mesodermal cell |
Present |
Regionally restricted |
1B
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Transient expression in mesodermal cells forming pericardial coelom.
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| TS11: embryo endoderm |
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Absent |
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1B
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No expression in endoderm.
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Specimen
1C:
embryonic day 7.5; Tnctm1Sia/Tnc +
(more )
Specimen
1C:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Tnctm1Sia/Tnc+
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS11: intraembryonic coelom |
mesodermal cell |
Present |
Regionally restricted |
1C
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Transient expression in mesodermal cells forming pericardial coelom.
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| TS11: embryo endoderm |
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Absent |
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1C
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No expression in endoderm.
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Specimen
2A:
embryonic day 9.5; Tnctm1Sia/Tnc +
(more )
Specimen
2A:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Tnctm1Sia/Tnc+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2B:
embryonic day 9.5; Tnctm1Sia/Tnc +
(more )
Specimen
2B:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Tnctm1Sia/Tnc+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2C:
embryonic day 9.5; Tnctm1Sia/Tnc +
(more )
Note: Double labeled: blue - Tnc reporter; brown - Tnc protein (assay MGI:7645232).
Specimen
2C:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Tnctm1Sia/Tnc+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: blue - Tnc reporter; brown - Tnc protein (assay MGI:7645232).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS15: heart |
fetal cardiomyocyte |
Present |
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2C
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Specimen
2D:
embryonic day 9.5; Tnctm1Sia/Tnc +
(more )
Note: Double labeled: blue - Tnc reporter; brown - Tnc protein (assay MGI:7645232).
Specimen
2D:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Tnctm1Sia/Tnc+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: blue - Tnc reporter; brown - Tnc protein (assay MGI:7645232).
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Specimen
2E:
embryonic day 9.5; Tnctm1Sia/Tnc +
(more )
Note: Double labeled: blue - Tnc; brown - sarcomeric myosin.
Specimen
2E:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Tnctm1Sia/Tnc+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: blue - Tnc; brown - sarcomeric myosin.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS15: outflow tract endocardial cushion |
fetal cardiomyocyte |
Present |
Regionally restricted |
2E
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Expression in cardiomyocytes also expressing sarcomeric myosin.
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Specimen
2F:
embryonic day 9.5; Tnctm1Sia/Tnc +
(more )
Note: Double labeled: blue - Tnc reporter; brown - Tnc protein (assay MGI:7645232).
Specimen
2F:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Tnctm1Sia/Tnc+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: blue - Tnc reporter; brown - Tnc protein (assay MGI:7645232).
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Specimen
2I:
embryonic day 9.5; Tnctm1Sia/Tnc +
(more )
Note: Double labeled: blue - Tnc reporter; brown - Tnc protein (assay MGI:7645232).
Specimen
2I:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Tnctm1Sia/Tnc+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: blue - Tnc reporter; brown - Tnc protein (assay MGI:7645232).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS15: pericardium |
cardiac mesenchymal cell |
Present |
Regionally restricted |
2I
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Expression in mesenchymal cells in proepicardial organs and pericardium.
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| TS15: proepicardium |
cardiac mesenchymal cell |
Present |
Regionally restricted |
2I
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Expression in mesenchymal cells in proepicardial organs and pericardium.
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| TS15: visceral pericardium |
mesothelial cell of epicardium |
Present |
Regionally restricted |
2I
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No expression in epicardial cells.
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| TS15: primitive ventricle cardiac muscle |
fetal cardiomyocyte |
Present |
Regionally restricted |
2I
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No expression in ventricular cardiomyocytes.
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Specimen
4A:
embryonic day 11.5; Tnctm1Sia/Tnc +
(more )
Note: Double labeled: blue - Tnc reporter; brown - Tnc protein (assay MGI:7645232).
Specimen
4A:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Tnctm1Sia/Tnc+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: blue - Tnc reporter; brown - Tnc protein (assay MGI:7645232).
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Specimen
4B:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Tnctm1Sia/Tnctm1Sia
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: blue - Tnc reporter; brown - Tnc protein (assay MGI:7645232).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: visceral pericardium |
Present |
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4B
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Expression pattern is same as that of heterozygous embryos.
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Specimen
4C:
embryonic day 11.5; Tnctm1Sia/Tnc +
(more )
Note: Double labeled: blue - Tnc reporter; brown - Tnxb (assay MGI:7645233).
Specimen
4C:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Tnctm1Sia/Tnc+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: blue - Tnc reporter; brown - Tnxb (assay MGI:7645233).
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Specimen
4D:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Tnctm1Sia/Tnctm1Sia
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: blue - Tnc reporter; brown - Tnxb (assay MGI:7645233).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: visceral pericardium |
Present |
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4D
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Expression pattern is same as that of heterozygous embryos.
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