Gene Expression Data
Assay Details
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Assay
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Reference:
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J:76800
North TE, et al., Runx1 expression marks long-term repopulating hematopoietic stem cells in the midgestation mouse embryo. Immunity. 2002 May;16(5):661-72
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7707700
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Gene symbol:
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Kit
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Gene name:
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KIT proto-oncogene receptor tyrosine kinase
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Antibody:
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anti-CD117 (2B8)
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
4B:
embryonic day 11.5; Runx1tm2Spe/Runx1 +
(more  )
Note: Double labeled: brown - Kit; blue - Runx1 reporter (assay MGI:7707694).
Specimen
4B:
(close  )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6J
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Mutant Allele(s):
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Runx1tm2Spe/Runx1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Double labeled: brown - Kit; blue - Runx1 reporter (assay MGI:7707694).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: aorta-gonad-mesonephros |
endothelial cell |
Present |
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4B
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Co-expression with Runx1 in endothelial cells
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| TS19: aorta-gonad-mesonephros |
mesenchymal cell |
Present |
Regionally restricted |
4B
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Co-expression with Runx1 in mesenchymal cells directly under the endothelium.
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| TS19: aorta-gonad-mesonephros |
hematopoietic stem cell |
Present |
Regionally restricted |
4B
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Co-expression with Runx1 in intraaortic hemaptopoietic clusters.
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Analysis Tools
