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Gene Expression Data
Assay Details
Assay
Reference: J:321836 Zhao K, et al., LONP1-mediated mitochondrial quality control safeguards metabolic shifts in heart development. Development. 2022 Mar 15;149(6):dev200458
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7711946
Gene symbol: Tnnt2
Gene name: troponin T2, cardiac
Antibody: Anti-Troponin T MS295P1 clone 13-11 (MA5-12960)
Detection system: Secondary antibody
Results
Specimen 1I Ctrl: embryonic day 13.5 (more )
Note: Double labeled: red - Tnnt2; green - Mki67 (assay MGI:7711942).
Structure Cell Type Level Pattern Image Note
TS21: right ventricle cardiac muscle fetal cardiomyocyte Present 1I Ctrl
Specimen 1I cKO: embryonic day 13.5; Lonp1tm1.1Zzy/Lonp1tm1.1Zzy, Tg(Mef2c-cre)2Blk/0 (more )
Note: Conditional mutant. Double labeled: red - Tnnt2; green - Mki67 (assay MGI:7711942).
Structure Cell Type Level Pattern Image Note
TS21: right ventricle cardiac muscle fetal cardiomyocyte Present 1I cKO Reduced proliferation was primarily in cardiomyocytes.
Specimen 2H/H' Ctrl: embryonic day 11.5 (more )
Note: Double labeled: red - Tnnt2; green - Mki67 (assay MGI:7711942).
Structure Cell Type Level Pattern Image Note
TS19: cardiac muscle tissue fetal cardiomyocyte Present 2H Ctrl , 2H'
Specimen 2H/H" cKO: embryonic day 11.5; Lonp1tm1.1Zzy/Lonp1tm1.1Zzy, Tg(Tnnt2-cre)5Blh/0 (more )
Note: Conditional mutant. Double labeled: red - Tnnt2; green - Mki67 (assay MGI:7711942).
Structure Cell Type Level Pattern Image Note
TS19: cardiac muscle tissue fetal cardiomyocyte Present 2H cKO , 2H" Significantly reduced cardiomyocyte proliferation was seen.
Specimen 3K Ctrl RV: embryonic day 11.5 (more )
Note: Double labeled: red - Tnnt2; green - H2ax (assay MGI:7711947).
Structure Cell Type Level Pattern Image Note
TS19: right ventricle cardiac muscle fetal cardiomyocyte Present 3K Ctrl RV
Specimen 3K Ctrl LV: embryonic day 11.5 (more )
Note: Double labeled: red - Tnnt2; green - H2ax (assay MGI:7711947).
Structure Cell Type Level Pattern Image Note
TS19: left ventricle cardiac muscle fetal cardiomyocyte Present 3K Ctrl LV
Specimen 3K cKO RV: embryonic day 11.5; Lonp1tm1.1Zzy/Lonp1tm1.1Zzy, Tg(Tnnt2-cre)5Blh/0 (more )
Note: Conditional mutant. Double labeled: red - Tnnt2; green - H2ax (assay MGI:7711947).
Structure Cell Type Level Pattern Image Note
TS19: right ventricle cardiac muscle fetal cardiomyocyte Present 3K cKO RV Significantly increased level of DNA damage (H2ax+) compared with the control.
Specimen 3K cKO LV: embryonic day 11.5; Lonp1tm1.1Zzy/Lonp1tm1.1Zzy, Tg(Tnnt2-cre)5Blh/0 (more )
Note: Conditional mutant. Double labeled: red - Tnnt2; green - H2ax (assay MGI:7711947).
Structure Cell Type Level Pattern Image Note
TS19: left ventricle cardiac muscle fetal cardiomyocyte Present 3K cKO LV Significantly increased level of DNA damage (H2ax+) compared with the control.
Specimen 5E E9.5 Ctrl: embryonic day 9.5 (more )
Structure Cell Type Level Pattern Image Note
TS15: cardiac muscle tissue fetal cardiomyocyte Present 5E E9.5 cTnT Ctrl
Specimen 5E E10.5 Ctrl: embryonic day 10.5 (more )
Structure Cell Type Level Pattern Image Note
TS17: cardiac muscle tissue fetal cardiomyocyte Present 5E E10.5 cTnT Ctrl
Specimen 5E E12.5 Ctrl: embryonic day 12.5 (more )
Structure Cell Type Level Pattern Image Note
TS20: cardiac muscle tissue fetal cardiomyocyte Present 5E E12.5 cTnT Ctrl
Specimen 5E E9.5 cKO: embryonic day 9.5; Lonp1tm1.1Zzy/Lonp1tm1.1Zzy, Tg(Tnnt2-cre)5Blh/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS15: cardiac muscle tissue fetal cardiomyocyte Present 5E E9.5 cTnT cKO
Specimen 5E E10.5 cKO: embryonic day 10.5; Lonp1tm1.1Zzy/Lonp1tm1.1Zzy, Tg(Tnnt2-cre)5Blh/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS17: cardiac muscle tissue fetal cardiomyocyte Present 5E E10.5 cTnT cKO
Specimen 5E E12.5 cKO: embryonic day 12.5; Lonp1tm1.1Zzy/Lonp1tm1.1Zzy, Tg(Tnnt2-cre)5Blh/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS20: cardiac muscle tissue fetal cardiomyocyte Present 5E E12.5 cTnT cKO
Specimen S5A Ctrl: embryonic day 14.5 (more )
Note: Double labeled: red - Tnnt2; green - Atf4 (assay MGI:7711996).
Structure Cell Type Level Pattern Image Note
TS22: right ventricle cardiac muscle fetal cardiomyocyte Present S5A Ctrl , S5A'
Specimen S5A cKO: embryonic day 14.5; Lonp1tm1.1Zzy/Lonp1tm1.1Zzy, Tg(Mef2c-cre)2Blk/0 (more )
Note: Conditional mutant. Double labeled: red - Tnnt2; green - Atf4 (assay MGI:7711996).
Structure Cell Type Level Pattern Image Note
TS22: right ventricle cardiac muscle fetal cardiomyocyte Present S5A" , S5A cKO
Specimen S6B Ctrl: embryonic day 10.5 (more )
Structure Cell Type Level Pattern Image Note
TS17: cardiac muscle tissue fetal cardiomyocyte Present S6B Ctrl cTnT
Specimen S6B cKO: embryonic day 10.5; Lonp1tm1.1Zzy/Lonp1tm1.1Zzy, Tg(Tnnt2-cre)5Blh/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS17: cardiac muscle tissue fetal cardiomyocyte Present S6B cKO cTnT
Specimen S6E Ctrl: embryonic day 10.5 (more )
Structure Cell Type Level Pattern Image Note
TS17: cardiac muscle tissue fetal cardiomyocyte Present S6E Ctrl cTnT
Specimen S6E cKO: embryonic day 10.5; Lonp1tm1.1Zzy/Lonp1tm1.1Zzy, Tg(Tnnt2-cre)5Blh/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS17: cardiac muscle tissue fetal cardiomyocyte Present S6E cKO cTnT
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/10/2024
MGI 6.24 		The Jackson Laboratory