Gene Expression Data
Assay Details
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Assay
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Reference:
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J:233757
Treffy RW, et al., Ephrin-B/EphB Signaling Is Required for Normal Innervation of Lingual Gustatory Papillae. Dev Neurosci. 2016;38(2):124-38
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7834501
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Gene symbol:
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Efnb1
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Gene name:
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ephrin B1
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Antibody:
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anti-Ephrin-B1
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Assay notes:
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Two Efnb1 antibodies were described, but the one used in this assay was not detailed. Anti-Ephrin-B1 (AF473) MGI:7834495 may have been used in this assay.
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Results
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Specimen
1a:
embryonic day 12.5
(more  )
Note: Double labeled: red - Efnb1; green - Uchl1 (assay MGI:7834502).
Specimen
1a:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Efnb1; green - Uchl1 (assay MGI:7834502).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS20: trunk mesenchyme |
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Strong |
Regionally restricted |
1a
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Strong expression in mesenchyme surrounding spinal cord.
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| TS20: dorsal root ganglion |
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Strong |
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1a
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Strong expression in dorsal root ganglia.
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| TS20: spinal cord |
radial glial cell |
Moderate |
Regionally restricted |
1a
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Moderate expression in dorsal radial glia.
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Specimen
1a'/a":
embryonic day 14.5
(more )
Note: Double labeled: red - Efnb1; green - Uchl1 (assay MGI:7834502).
Specimen
1a'/a":
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Efnb1; green - Uchl1 (assay MGI:7834502).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: tongue mesenchyme |
Present |
Regionally restricted |
1a'
,
1a"
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Expression in band of mesenchyme beneath epithelium.
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| TS22: fungiform papillae |
Trace |
Regionally restricted |
1a"
,
1a'
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Expression barely detectable in lingual epithelium, including fungiform papillae.
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| TS22: tongue epithelium |
Trace |
Regionally restricted |
1a"
,
1a'
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Expression barely detectable in lingual epithelium, including fungiform papillae.
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